Group of tumor diagnosis markers, and application thereof

A technology of tumor diagnosis and effector cells, applied in the field of disease diagnosis, to achieve the effects of easy material collection, less pain for patients, and high diagnostic efficiency

Pending Publication Date: 2021-05-07
JINSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, no CD3 - CD19 - CD16 + NK cell ratio, CD3 - CD19 - CD16 - NK cell ratio, TH CM ratio or TH effector cell ratio can be used as tumor markers to distinguish healthy people from tumor patients reported

Method used

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  • Group of tumor diagnosis markers, and application thereof
  • Group of tumor diagnosis markers, and application thereof
  • Group of tumor diagnosis markers, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Collect clinical samples

[0032] From May 2020 to August 2020, we collected a total of 474 samples from the Department of Oncology and Laboratory of Fudan University Jinshan Hospital, including 288 blood samples from normal immune patients and 186 blood samples from cancer patients.

[0033]The age distribution of the sample population in the two groups was between 18 and 70 years old; cancer patients included 50 cases of esophageal cancer, 26 cases of gastrointestinal cancer, 60 cases of breast cancer, and 50 cases of lung cancer; Infections, autoimmune diseases, tumors and other diseases that have been confirmed to affect the immune status were excluded.

[0034] 2. Preparation and detection of fresh sample detection marker samples

[0035] Take fresh anticoagulated whole blood in a 1.5 ml centrifuge tube, separate the serum after centrifugation and store it in a low-temperature refrigerator. Add red blood cell lysate 6 times the volume of the cell pellet, and l...

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PUM

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Abstract

The invention relates to a group of tumor diagnosis markers, and application thereof. Immune cells in 288 normal blood samples and 186 cancer patients in clinic are analyzed, and it is found that the proportion of CD3<->CD19<->CD16<+>NK cells, the proportion of CD3<->CD19<->CD16<->NK cells, the proportion of TH CM and the proportion of TH effector cells are significantly different in a healthy group and a tumor group, and can be used as a diagnostic index for distinguishing healthy people from tumor patients, and AUC is more than 0.96. The invention provides a novel kit for tumor diagnosis. The kit can be used for clinical tumor detection, and is particularly suitable for tumor screening in physical examination items.

Description

technical field [0001] The present invention relates to the field of disease diagnosis, in particular to a group of tumor diagnostic markers and applications thereof. Background technique [0002] The body's cellular immune function is closely related to the occurrence and development of tumors. [0003] Literature (Liu Yan, Zhu Meiqi. The role of soluble IL-2R, IL-6 and T cell subsets in the diagnosis of benign and malignant ovarian tumors [J]. Practical Tumor Journal, 2001.) published: ELISA method, indirect The peripheral blood T lymphocyte subsets of the patients were determined by immunofluorescence method, and the results showed that the peripheral blood CD3 of all patients + and CD8 + No significant changes, CD4 in 13 of 17 ovarian malignancies + The cells were significantly reduced, and the ratio of CD4 / CD8 was significantly decreased, which was significantly different from that in the control group and benign ovarian lesions. [0004] Literature (Qian Xiaoqin, L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N15/14
CPCG01N15/14G01N33/56966G01N33/57488G01N2015/1486
Inventor 武多娇程韵枫乔田奎李云春王向东杨莹杨志诚
Owner JINSHAN HOSPITAL FUDAN UNIV
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