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A Microfluidic Chip for Enriching Cells

A microfluidic chip and chip technology, which is applied in the direction of laboratory containers, laboratory utensils, chemical instruments and methods, etc., can solve the problems of large consumption of reagents, easy loss, high cost, etc., so as to reduce the consumption of reagents , improve binding efficiency and reduce sample loss

Active Publication Date: 2022-07-08
BEIJING MECHANICAL EQUIP INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above methods also have technical bottlenecks, such as low throughput, easy loss in the operation process, large consumption of reagents, high cost, etc.

Method used

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  • A Microfluidic Chip for Enriching Cells
  • A Microfluidic Chip for Enriching Cells
  • A Microfluidic Chip for Enriching Cells

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0031] Attached below Figures 1 to 5 The microfluidic chip provided by the present invention is described in detail as follows:

[0032] figure 1 and figure 2 Chips in two different packaging modes are shown. One of the packaging structures such as figure 1 As shown: the upper chip, the middle chip and the lower chip are semi-reversible encapsulation, the semi-reversible encapsulation includes bonding the lower chip 1 and the encapsulation sheet 11 for irreversible encapsulation, and the size of the upper chip and the middle chip is sufficient to cover The encapsulation sheet 11 is opened.

[0033] Another package structure such as figure 2 As shown: the upper chip 3, the middle chip 2 and the lower chip 1 are reversibly packaged, and the upper surface 21 and the lower surface 22 of the middle chip are respectively coated with 0.05-0.5mm elastic layer, and use parylene (parylene) hydrophobic treatment is performed on the surface of the chip, and the lower-layer chip 1...

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Abstract

The present invention provides a microfluidic chip for enriching cells, the chip includes a lower layer chip 1, a middle layer chip 2 and an upper layer chip 3; the lower layer chip 1 includes an elution module 105, a buffer module 106, a specific binding module 107 and enrichment module 108; between the elution module 105 and the buffer module 106 is provided with a water-blocking gas permeable membrane 104; the elution module 105 is provided with a vertical buffer inlet 101; The channel is provided with a specific magnetic particle solution inlet 103 ; the middle-layer chip includes a filter module 201 and a cavity area 202 corresponding to the water-blocking gas-permeable membrane 104 . The present invention utilizes the multi-channel technology to achieve high throughput while being compatible with the flow restriction of subsequent chip modules, thereby reducing the sample loss of the connection of each module.

Description

technical field [0001] The invention relates to a microfluidic chip and medical detection technology, in particular to a microfluidic chip and a method for capturing circulating tumor cells by using the same. Background technique [0002] Circulating tumor cells, CTC and circulating tumor microemboli, CTM in peripheral blood are considered to be important causes and markers of tumor metastasis and recurrence. Its presence represents both the invasive capacity of the primary tumor and the possibility of forming metastases at distant sites. Therefore, accurate detection of the number of CTCs and CTMs in blood per unit volume can provide an important basis for early screening of cancer; provide dynamic monitoring and prognostic basis for the development of tumor patients; at the same time, it is also the pathogenic mechanism and drug resistance mechanism of tumor cells. window of study. Compared with other normal blood cells in peripheral blood, the number of CTCs and CTMs pe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/502761B01L2200/12
Inventor 许诺王一凡王忠晶刘立滨
Owner BEIJING MECHANICAL EQUIP INST
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