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Medium composition for suspension culture of adhesive cells

A technology for adherent cells and suspension culture, which is applied in the field of medium composition for suspension culture of adherent cells, can solve problems such as cell damage and reduced cell survival rate, and achieve the effect of high-efficiency production and simple culture scale

Pending Publication Date: 2021-05-28
NISSAN CHEM IND LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, another problem is that when cells are recovered from microcarriers for the purpose of changing the medium or expanding the scale of culture, etc., it is necessary to detach between cells, cells and carriers, and proteolysis such as trypsin used in this detachment process Enzymes can damage cells, resulting in reduced cell viability

Method used

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  • Medium composition for suspension culture of adhesive cells
  • Medium composition for suspension culture of adhesive cells
  • Medium composition for suspension culture of adhesive cells

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preparation example Construction

[0108] A method for producing nanofibers is described in WO2015 / 111686A1 and the like.

[0109] Polysaccharides may be added to the medium composition of the present invention. In this specification, polysaccharides mean sugar polymers in which 10 or more monosaccharides (such as three-carbon sugars, four-carbon sugars, five-carbon sugars, six-carbon sugars, and seven-carbon sugars) are polymerized.

[0110] Examples of water-insoluble polysaccharides include celluloses such as cellulose and hemicellulose; chitins such as chitin and chitosan, and the like, but are not limited to these.

[0111]Examples of water-soluble polysaccharides include acidic polysaccharides having anionic functional groups. The acidic polysaccharides having an anionic functional group are not particularly limited, for example, uronic acid (for example, glucuronic acid, iduronic acid, galacturonic acid, mannuronic acid) in the structure can be mentioned. acid), polysaccharides having sulfuric acid or ...

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Abstract

Provided is a medium composition for suspension culture of adhesive cells, said medium composition comprising: (1) chitin nanofibers; and (2) chitosan nanofibers or a polysaccharide.

Description

technical field [0001] The present invention relates to a medium composition for suspension culture of adherent cells and a method for suspension culture of adherent cells. More specifically, the present invention relates to a medium composition for suspension culture of adherent cells comprising chitin nanofibers and chitosan nanofibers, and an adhesive cell using the medium composition. Suspension culture method of sex cells. Background technique [0002] In the field of regenerative medicine, the construction of organ regeneration means using pluripotent stem cells such as iPS cells and ES cells has been intensively studied. However, the establishment of ES cells is accompanied by ethical issues, and the risk of canceration of iPS cells and the problems of long culture times have also been recognized. Therefore, methods using adult stem cells such as mesenchymal stem cells and neural stem cells with a low risk of cancerization, proadipocytes and progenitor cells such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12P1/00C12N5/071C12P21/00
CPCC12N5/0018C12N2501/90C12N2533/72C12N2533/74C12N2533/78C12N5/0663C12P21/00C12P1/00C12N5/0667C12N2533/70C12N2513/00C12N2500/46
Inventor 金木达朗木田克彦南昌孝畑中大辅
Owner NISSAN CHEM IND LTD
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