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An anti-Alzheimer's disease drug screening method based on behavior and biochemical indicators and a drug screened by the method

A biochemical index and screening method technology, applied in the field of drug screening, can solve the problems of scattered testing process, inability to batch test, single angle, etc., and achieve the effect of reducing system errors, shortening drug screening time, and easy operation.

Pending Publication Date: 2021-06-01
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the current one-sided, single-angle, fragmented, cumbersome testing process, heavy workload, and incapable of batch testing in Alzheimer's disease drug screening, the purpose of the present invention is to provide an anti-Alzheimer's drug based on behavioral and biochemical indicators. The screening method of Alzheimer's disease drug and the drug screened by the method can carry out comprehensive, high-efficiency and accurate screening of anti-Alzheimer's disease drug

Method used

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  • An anti-Alzheimer's disease drug screening method based on behavior and biochemical indicators and a drug screened by the method
  • An anti-Alzheimer's disease drug screening method based on behavior and biochemical indicators and a drug screened by the method
  • An anti-Alzheimer's disease drug screening method based on behavior and biochemical indicators and a drug screened by the method

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Experimental program
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Embodiment 1

[0110] Construction of Alzheimer's disease model in juvenile zebrafish

[0111] 1.1 Experimental materials

[0112] Aluminum chloride, produced by Shanghai Aladdin Company. Healthy juvenile zebrafish (3 days old) were provided by the Institute of Biology, Shandong Academy of Sciences, and strictly followed the relevant processing guidelines of the International Laboratory Animal Protection Certification and Evaluation Agency.

[0113] 1.2 Experimental method

[0114] 1.1.1 Experiment preparation:

[0115] Select 3-day-old zebrafish juveniles and divide them into eight groups randomly, with 30 fish in each group. Place the eight groups of fish in a 24-well cell culture plate (8 to 12 fish in each well), and keep the pH at 7.0 to 7.5. The water environment was kept in a constant temperature incubator with an ambient temperature of 28.5°C, and it was always maintained in an alternating day and night environment with 10 hours of light and 14 hours of shading.

[0116] 1.2.1 Ex...

Embodiment 2

[0124] A method for screening anti-Alzheimer's disease drugs based on behavioral and biochemical indicators, comprising the following steps:

[0125] (1) Construction of Alzheimer's disease model in juvenile zebrafish

[0126] 1.1 Experimental materials

[0127] Aluminum chloride, produced by Shanghai Aladdin Company. Healthy juvenile zebrafish (3 days old) were provided by the Institute of Biology, Shandong Academy of Sciences, and strictly followed the relevant processing guidelines of the International Laboratory Animal Protection Certification and Evaluation Agency.

[0128] 1.2 Experimental method

[0129] Using 3-day-old zebrafish larvae as a model animal, pass 80 μM AlCl 3 Solution induction, establishment of Alzheimer's disease model in zebrafish larvae;

[0130] Specifically, 80 μM AlCl was added to the wells of the 24-well cell culture plate 3 solution, put 3-day-old zebrafish juveniles, 10 fish in each well, keep the water environment with a pH of 7.0-7.5, and ...

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Abstract

The invention discloses an anti-Alzheimer's disease drug screening method based on behavior and biochemical indicators and a drug screened by the method. The screening method comprises the steps that a zebra fish juvenile fish Alzheimer's disease model is built, the maximum safe concentration of a drug to be screened is determined, and experimental treatment is conducted on zebra fish juvenile fish; the method also includes screening an anti-Alzheimer's disease drug by utilizing behavioral and biochemical indexes; and judging whether the medicine has the Alzheimer's disease resistance or not. According to the anti-Alzheimer's disease drug screening method based on behavior and biochemical indicators, the anti-Alzheimer's disease drug can be systematically and completely screened, especially, new drugs with uncertain efficacy are screened; compared with the prior art, medicine soaking and testing environments are simpler and only need to be carried out in the experimental pore plate, multiple fishes can be soaked in medicine in one pore during medicine soaking, the behavior ability of 48 fishes can be tested at most at the same time during testing, the operation process is simple and easy to operate, the testing workload is greatly reduced, and testing system errors are reduced.

Description

technical field [0001] The invention relates to the technical field of drug screening, in particular to a screening method for anti-Alzheimer's disease drugs based on behavioral and biochemical indicators and the drug screened by the method. Background technique [0002] Alzheimer's disease is the most common neurodegenerative disease that plagues middle-aged and elderly people. Memory and cognitive dysfunction are the core features of the disease. decline, unresponsiveness, and even personality changes that progress to a state of general dementia. The main neuropathological features of Alzheimer's disease are hyperphosphorylated tau protein aggregation leading to intracellular neurofibrillary tangles; abnormal accumulation of β-amyloid protein to form extracellular amyloid plaque; due to excessive aggregation of abnormal proteins in brain regions, It affects and even apoptosis brain cells, forming the typical neuropathological damage of Alzheimer's disease patients. On th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K36/28A61P25/28
CPCA61K49/0008A61K36/28A61P25/28
Inventor 靳梦刘可春商学良任擎宇王利振盛文龙王荣春
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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