A kind of human t-lymphoblastic leukemia/lymphoma cell line and its application
A technology for lymphoma cells and blast cells, which is applied in the fields of biology and oncology, can solve the problems of restriction and no establishment of cell lines, and achieve the effect of shape stability
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example 1ZY
[0025] Example 1 ZYXY-T1 cell line preparation
[0026] Primary cell culture: Take 6ml of peripheral blood samples (male, male) obtained from the First Affiliated Hospital of Zhejiang University School of Medicine to separate leukemia mononuclear cells immediately. In a biosafety cabinet, take 6ml of peripheral blood samples dropwise into a 15ml sterile centrifuge tube pre-added with 6ml of lymphocyte separation medium, and centrifuge at 2000 rpm for 20 minutes. After centrifugation, take the mononuclear cell layer into a new 15ml sterile centrifuge tube, add 5ml sterile 1xPBS to resuspend the cells, and centrifuge at 2000 rpm for 5 minutes. After discarding the supernatant, add sterile erythrocyte lysate to lyse the cells at room temperature for 5 minutes, then centrifuge at 2000 rpm for 5 minutes. Discard the supernatant, add 5ml IMDM complete medium (IMDM 90% + fetal bovine serum 10%) to resuspend the cells, centrifuge at 1500 rpm for 5 minutes. Discard the supernatant, a...
example 2
[0029] Biological properties and application of example 2 human T lymphoblastic leukemia / lymphoma cell line
[0030] The invention adopts the IMDM medium containing 10% fetal bovine serum to cultivate the cell ZYXY-T1, so that it can grow stably in vitro and be passed down stably. Observed under the microscope, the cells are suspended single growth, round or oval. Wright-Giemsa staining showed that the cells were acute leukemia blasts with large and deeply stained nuclei. Flow cytometric analysis found that the cell line has the typical surface antigen characteristics of ETP-ALL, and it is the first ETP-ALL cell line established in the world. The cell line can be used for ETP-ALL pathogenesis research, screening and / or evaluation / preparation of tumor therapeutic drugs; development of tumor drug targets; preparation of tumor diagnostic products; screening of tumor biotherapeutic drugs / reagents; development and detection of tumor-related bioengineering products. details as fol...
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