A kind of human t-lymphoblastic leukemia/lymphoma cell line and its application

A technology for lymphoma cells and blast cells, which is applied in the fields of biology and oncology, can solve the problems of restriction and no establishment of cell lines, and achieve the effect of shape stability

Active Publication Date: 2021-11-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research of ETP-ALL is also inseparable from cell lines, but at present, no ETP-ALL cell lines have been established from ETP-ALL patient specimens at home and abroad
This has brought great constraints to the basic research and related clinical research of ETP-ALL

Method used

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  • A kind of human t-lymphoblastic leukemia/lymphoma cell line and its application
  • A kind of human t-lymphoblastic leukemia/lymphoma cell line and its application
  • A kind of human t-lymphoblastic leukemia/lymphoma cell line and its application

Examples

Experimental program
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example 1ZY

[0025] Example 1 ZYXY-T1 cell line preparation

[0026] Primary cell culture: Take 6ml of peripheral blood samples (male, male) obtained from the First Affiliated Hospital of Zhejiang University School of Medicine to separate leukemia mononuclear cells immediately. In a biosafety cabinet, take 6ml of peripheral blood samples dropwise into a 15ml sterile centrifuge tube pre-added with 6ml of lymphocyte separation medium, and centrifuge at 2000 rpm for 20 minutes. After centrifugation, take the mononuclear cell layer into a new 15ml sterile centrifuge tube, add 5ml sterile 1xPBS to resuspend the cells, and centrifuge at 2000 rpm for 5 minutes. After discarding the supernatant, add sterile erythrocyte lysate to lyse the cells at room temperature for 5 minutes, then centrifuge at 2000 rpm for 5 minutes. Discard the supernatant, add 5ml IMDM complete medium (IMDM 90% + fetal bovine serum 10%) to resuspend the cells, centrifuge at 1500 rpm for 5 minutes. Discard the supernatant, a...

example 2

[0029] Biological properties and application of example 2 human T lymphoblastic leukemia / lymphoma cell line

[0030] The invention adopts the IMDM medium containing 10% fetal bovine serum to cultivate the cell ZYXY-T1, so that it can grow stably in vitro and be passed down stably. Observed under the microscope, the cells are suspended single growth, round or oval. Wright-Giemsa staining showed that the cells were acute leukemia blasts with large and deeply stained nuclei. Flow cytometric analysis found that the cell line has the typical surface antigen characteristics of ETP-ALL, and it is the first ETP-ALL cell line established in the world. The cell line can be used for ETP-ALL pathogenesis research, screening and / or evaluation / preparation of tumor therapeutic drugs; development of tumor drug targets; preparation of tumor diagnostic products; screening of tumor biotherapeutic drugs / reagents; development and detection of tumor-related bioengineering products. details as fol...

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Abstract

The invention discloses a human T-lymphoblastic leukemia / lymphoma cell line and its construction method and application. The human T-lymphoblastic leukemia / lymphoma cell line is named as human T-lymphoblastic leukemia / lymphoma ZYXY-T1, and was deposited in the China Center for Type Culture Collection (Wuhan University, Wuhan, China) on January 20, 2021, with a deposit number It is CCTCC NO:C202143. The present invention is obtained by extracting and separating mononuclear cells from the peripheral blood of clinical human T-lymphoblastic leukemia / lymphoma patients, and culturing them in vitro for continuous natural passage. This leukemia cell line has the typical surface antigen expression characteristics of ETP‑ALL, that is, it does not express CD1α, CD5, CD8, highly expresses the stemness marker CD34, has good in vitro proliferation ability and in vivo tumorigenesis ability, and can be used as a study on the development mechanism of ETP‑ALL Research and individualized treatment In vitro research cell materials can also be used for in vivo and in vitro research ETP‑ALL drug screening, evaluation, and guidance for clinical medication.

Description

technical field [0001] The invention relates to the fields of biology and oncology, and relates to a human T-lymphoblastic leukemia / lymphoma cell line and its construction method and application Background technique [0002] Acute lymphoblastic leukemia (ALL) is a hematological malignancy of malignant clonal proliferation of immature lymphocytes, which is more common in children, and the incidence rate of adults is lower than that of children. ALL is divided into B-cell ALL (B-ALL) , accounting for about 85% of the number of patients, T-cell (T-ALL) accounts for about 15% of the number of patients. T-ALL is a high-risk type of ALL. Although combined chemotherapy can achieve a remission rate of 90% to 95%, nearly one-third of patients relapse, and the five-year overall survival rate is about 50%. For initial refractory and relapsed patients, the current treatment options are very limited, and the prognosis of patients is extremely poor. [0003] T-lymphoblastic leukemia / lym...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09C12Q1/02
CPCC12N5/0694G01N33/5011G01N33/5047C12N2503/02
Inventor 金洁李枫林王华锋主鸿鹄黄昕张仪胡超
Owner ZHEJIANG UNIV
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