A kind of human t-lymphoblastic leukemia/lymphoma cell line and its application

A technology for lymphoma cells and blast cells, which is applied in the fields of biology and oncology, can solve the problems of restriction and no establishment of cell lines, and achieve the effect of shape stability
CN112877290BActive Publication Date: 2021-11-30ZHEJIANG UNIV
6 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2021-11-30

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a human T-lymphoblastic leukemia / lymphoma cell line and its construction method and application. The human T-lymphoblastic leukemia / lymphoma cell line is named as human T-lymphoblastic leukemia / lymphoma ZYXY-T1, and was deposited in the China Center for Type Culture Collection (Wuhan University, Wuhan, China) on January 20, 2021, with a deposit number It is CCTCC NO:C202143. The present invention is obtained by extracting and separating mononuclear cells from the peripheral blood of clinical human T-lymphoblastic leukemia / lymphoma patients, and culturing them in vitro for continuous natural passage. This leukemia cell line has the typical surface antigen expression characteristics of ETP‑ALL, that is, it does not express CD1α, CD5, CD8, highly expresses the stemness marker CD34, has good in vitro proliferation ability and in vivo tumorigenesis ability, and can be used as a study on the development mechanism of ETP‑ALL Research and individualized treatment In vitro research cell materials can also be used for in vivo and in vitro research ETP‑ALL drug screening, evaluation, and guidance for clinical medication.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the fields of biology and oncology, and relates to a human T-lymphoblastic leukemia / lymphoma cell line and its construction method and application Background technique

[0002] Acute lymphoblastic leukemia (ALL) is a hematological malignancy of malignant clonal proliferation of immature lymphocytes, which is more common in children, and the incidence rate of adults is lower than that of children. ALL is divided into B-cell ALL (B-ALL) , accounting for about 85% of the number of patients, T-cell (T-ALL) accounts for about 15% of the number of patients. T-ALL is a high-risk type of ALL. Although combined chemotherapy can achieve a remission rate of 90% to 95%, nearly one-third of patients relapse, and the five-year overall survival rate is about 50%. For initial refractory and relapsed patients, the current treatment options are very limited, and the prognosis of patients is extremely poor.

[0003] T-lymphoblastic leukemia / lym...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More