Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel coronavirus antigen and its application

An anti-antibody and amino acid technology, applied in the field of biomedicine, can solve the problems of low signal value and missed detection, and achieve the effect of not easy to miss detection, high sensitivity and specificity

Active Publication Date: 2022-06-03
SHENZHEN YHLO BIOTECH
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing technology usually recombinantly expresses the full length of the new coronavirus S1 subunit, but when using the recombinant protein as the detection antigen, the signal value generated is low
However, if the RBD domain at the C-terminal of the new coronavirus S1 subunit is expressed alone, it is easy to cause missed detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel coronavirus antigen and its application
  • Novel coronavirus antigen and its application
  • Novel coronavirus antigen and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Embodiment 2

[0100] The mammalian cells HEK293F were recovered and cultured at a cell density of 1.5-2.5×10 prior to transfection

[0101] The 2019-nCoV-spike recombinant antigen expression product was filtered using a 0.45 μm filter, and loaded to a pre-equilibrated

Embodiment 3

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The present invention relates to the field of biomedicine, in particular to a novel coronavirus antigen and its application. The polypeptide comprises the following parts: S1-NTD—Linker—Avi-tag—Linker—S1-RBD. The NTD domain and the RBD domain of the S1 protein are connected in series through two repeated linker sequences, so that the two domains have better spatial extension to recognize antibodies; in addition, Avi‑ is introduced between the two linker sequences tag. The AviTag tag protein allows the recombinant protein to be immobilized through a fixed site, reducing the impact of steric hindrance caused by the coating process. Therefore, the peptide can achieve high sensitivity and specificity, and it is not easy to miss detection.

Description

Novel coronavirus antigen and its application technical field The present invention relates to the field of biomedicine, in particular, to a kind of novel coronavirus antigen and application thereof. Background technique [0002] Coronaviruses (CoVs; subfamily Coronaviridae, Coronaviridae, Viridae) are a highly diverse group of Membrane, positive-sense, single-stranded RNA virus that causes respiratory, intestinal, hepatic and nervous systems of varying severity in a variety of animals diseases, including humans. Coronaviruses are divided into four genera: alphacoronavirus, betacoronavirus (βCoV), gammacoronavirus and deltacoronavirus virus. In the past 12 years, two novel beta-coronaviruses, severe acute respiratory syndrome, have emerged (SARS‑CoV) and Middle East Respiratory Syndrome (MERS‑CoV), two viruses that cause severe human disease. Novel coronavirus (SARS-CoV-2), completed viral nucleic acid detection on January 10, and shared this genome information wit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/165C12N15/50C07K19/00C12N15/62G01N33/569G01N33/558G01N33/543G01N33/68
CPCC07K14/005G01N33/6854G01N33/56983G01N33/558G01N33/543C12N2770/20022C07K2319/02C07K2319/40C07K2319/00G01N2469/20
Inventor 林晓涛钱纯亘刘笔锋陈鹏林军李一荣胡鹍辉
Owner SHENZHEN YHLO BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com