Novel coronavirus antigen and its application

An anti-antibody and amino acid technology, applied in the field of biomedicine, can solve the problems of low signal value and missed detection, and achieve the effect of not easy to miss detection, high sensitivity and specificity

Active Publication Date: 2022-06-03
SHENZHEN YHLO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing technology usually recombinantly expresses the full length of the new coronavirus S1 subunit, but when using the recombinant protein as the detection antigen, the signal value generated is low
However, if the RBD domain at the C-terminal of the new coronavirus S1 subunit is expressed alone, it is easy to cause missed detection

Method used

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  • Novel coronavirus antigen and its application
  • Novel coronavirus antigen and its application
  • Novel coronavirus antigen and its application

Examples

Experimental program
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Effect test

Embodiment 1

Embodiment 2

[0100] The mammalian cells HEK293F were recovered and cultured at a cell density of 1.5-2.5×10 prior to transfection

[0101] The 2019-nCoV-spike recombinant antigen expression product was filtered using a 0.45 μm filter, and loaded to a pre-equilibrated

Embodiment 3

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Abstract

The present invention relates to the field of biomedicine, in particular to a novel coronavirus antigen and its application. The polypeptide comprises the following parts: S1-NTD—Linker—Avi-tag—Linker—S1-RBD. The NTD domain and the RBD domain of the S1 protein are connected in series through two repeated linker sequences, so that the two domains have better spatial extension to recognize antibodies; in addition, Avi‑ is introduced between the two linker sequences tag. The AviTag tag protein allows the recombinant protein to be immobilized through a fixed site, reducing the impact of steric hindrance caused by the coating process. Therefore, the peptide can achieve high sensitivity and specificity, and it is not easy to miss detection.

Description

Novel coronavirus antigen and its application technical field The present invention relates to the field of biomedicine, in particular, to a kind of novel coronavirus antigen and application thereof. Background technique [0002] Coronaviruses (CoVs; subfamily Coronaviridae, Coronaviridae, Viridae) are a highly diverse group of Membrane, positive-sense, single-stranded RNA virus that causes respiratory, intestinal, hepatic and nervous systems of varying severity in a variety of animals diseases, including humans. Coronaviruses are divided into four genera: alphacoronavirus, betacoronavirus (βCoV), gammacoronavirus and deltacoronavirus virus. In the past 12 years, two novel beta-coronaviruses, severe acute respiratory syndrome, have emerged (SARS‑CoV) and Middle East Respiratory Syndrome (MERS‑CoV), two viruses that cause severe human disease. Novel coronavirus (SARS-CoV-2), completed viral nucleic acid detection on January 10, and shared this genome information wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/165C12N15/50C07K19/00C12N15/62G01N33/569G01N33/558G01N33/543G01N33/68
CPCC07K14/005G01N33/6854G01N33/56983G01N33/558G01N33/543C12N2770/20022C07K2319/02C07K2319/40C07K2319/00G01N2469/20
Inventor 林晓涛钱纯亘刘笔锋陈鹏林军李一荣胡鹍辉
Owner SHENZHEN YHLO BIOTECH
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