Strain for promoting attachment and metamorphosis of shellfish larvae and application

A technology of attachment metamorphosis and strains, applied in the field of microorganisms, can solve the problem of less research on the induction of natural factors, and achieve the effect of increasing the attachment metamorphosis rate, strong applicability, and improving the attachment rate.

Active Publication Date: 2021-06-11
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Strain for promoting attachment and metamorphosis of shellfish larvae and application
  • Strain for promoting attachment and metamorphosis of shellfish larvae and application
  • Strain for promoting attachment and metamorphosis of shellfish larvae and application

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Embodiment 1

[0016] This embodiment relates to the isolation and identification of the strain that promotes the attachment of shellfish larvae to metamorphosis, including the following steps:

[0017] (1) Collect surface sediment samples from shellfish farming areas, mix the samples with sterile distilled water at a ratio of 1g:1mL, and after standing for 30 minutes, take the supernatant for gradient dilution, and then absorb 100 μL of the diluted liquid to apply Spread on LB solid plates, culture at 37°C for 12 h, then separate by streaking, repeat several times until a single colony is obtained after purification. The purified strains were inoculated on liquid LB medium, cultured at 37°C for 12 hours, stored in glycerol with a final concentration of 30%, and frozen at -80°C for later use.

[0018] LB liquid medium components are: peptone 10g, yeast powder 5g, NaCl 10g, distilled water 1000mL;

[0019] LB solid plate components are: peptone 10g, yeast powder 5g, NaCl 10g, agar powder 12g...

Embodiment 2

[0030] This embodiment relates to the above-mentioned application of promoting shellfish larvae to adhere to metamorphic strains, including:

[0031] (1) Pseudoalteromonas sp. Correlation analysis between biofilm formation ability and attachment metamorphosis rate of abalone seedlings; Pseudoalteromonas sp. The analysis chart of biofilm formation ability is shown in figure 1 shown, found Pseudoalteromonas sp. The biofilm formation ability was initial concentration dependent (Panel A). And the initial bacterial inoculum concentration was 1 × 10 7 When CFU / mL, the attachment rate of abalone larvae was the highest, about 1 × 10 3 2.67 times that of the CFU / mL group (Panel B). The above results show that Pseudoalteromonas sp. The induction effect on metamorphosis rate of abalone larvae may be related to its biofilm formation. The specific connection needs further research and excavation; figure 1 , at different starting concentrations, Pseudoalteromonas sp. Biofi...

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Abstract

The invention provides a strain for promoting attachment and metamorphosis of shellfish larvae and application. The strain is classified and named as Pseudoalteromonas sp., the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 8, 2020, the preservation address is Beijing, China, and the preservation number is CGMCC NO.20040. The invention further relates to application of the strain in the attachment, metamorphosis and development process of shellfish seedlings. The strain is adopted for induction, so that the attachment and metamorphosis rate of haliotis discus hannai seedlings can be increased. There is no toxic or side effect, and no influence is caused on haliotis discus hannai. The strain can be matched with other active substances for use, so that the attachment rate of the haliotis discus hannai seedlings is increased. The strain is high in operability, convenient, simple and high in practical applicability.

Description

technical field [0001] The invention belongs to the technical field of microorganisms; in particular, it relates to a bacterial strain for promoting the attachment of shellfish larvae to metamorphosis and its application. Background technique [0002] The planktonic larvae of many marine benthic invertebrates must attach and metamorphose after ending their planktonic life. The attachment and metamorphosis of larvae usually require certain substances to induce. Currently, a variety of chemical substances have been proven to induce metamorphosis in larvae, including neuroactive substances, petroleum products, organic solvents, and inorganic compounds. However, there are few studies on the induction of natural factors. In comparison, the induction of natural factors may be closer to the life and development characteristics of the larvae themselves, and has the characteristics of safety, environmental protection, and high efficiency. [0003] At present, studies have pointed ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01K61/51C12R1/01
CPCA01K61/51Y02A40/81
Inventor 杨顶珑吕成杰赵建民
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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