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Kit for detecting oxidation resistance genotype of human skin and method thereof

A technology of antioxidant capacity and genotype, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc.

Inactive Publication Date: 2021-06-11
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, all kinds of anti-oxidant skin care products on the market have different labels for different effects. Although a large number of tests have been carried out, there are still certain limitations.

Method used

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  • Kit for detecting oxidation resistance genotype of human skin and method thereof
  • Kit for detecting oxidation resistance genotype of human skin and method thereof
  • Kit for detecting oxidation resistance genotype of human skin and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039](1) Selected samples: exfoliated cell samples from the oral cavity wall of subject 1 and subject 2.

[0040] (2) Sample collection

[0041] Collection type: Exfoliated cells from the inner wall of the oral cavity.

[0042] Collection method: Use the saliva collection stick to wipe back and forth on the inner wall of the oral cavity 4 times, continue to wipe back and forth on the inner wall of the oral cavity 4 times with the reverse side of the saliva collection stick, take out the saliva collection stick, and press repeatedly on the saliva sample collection card to collect the saliva inner wall cells Transfer to the saliva sample collection card, and dry the collected saliva sample collection card in a pollution-free area.

[0043] Valid sample: The area where the pink area of ​​the saliva sample collection card changes to light pink or white is the valid saliva sample area.

[0044] Sample selection method: use Dubbo plastic puncher (1.0mm) for manual punching and sa...

Embodiment 2

[0064] (1) Selected sample: the blood sample of subject 1.

[0065] (2) Sample type: blood sample.

[0066] (3) DNA extraction: DNA extraction is performed on the blood sample using a nucleic acid extraction instrument.

[0067] (4) System configuration

[0068] The PCR reaction solution and the primers Primer Mix A and Primer Mix B were configured according to the proportion of the instruction manual. After vortex mixing, centrifuge with a centrifuge, and then use a pipette gun to distribute according to volume.

[0069] (5) Adding samples: According to the instructions, take a certain amount of extracted DNA samples with a pipette and add them to the reaction system.

[0070] (6) Amplification program

[0071] The amplification program on the PCR instrument is shown in Table 2.

[0072] (7) The amplified product is detected on the 3500DX genetic analyzer

[0073] The sample loading mixture is composed of deionized formamide and molecular weight internal standard (Size-5...

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Abstract

The invention discloses a kit for detecting oxidation resistance genotype of human skin and a methodthereof. The invention adopts multiple PCR amplification and electrophoresis methods to analyze and identify six allele polymorphism (SNP) sites in four genes (NQ01, SOD2, NFE2L2 and GPX1). The method comprises the following steps: a) collecting exfoliated cells in the oral cavity of a testee, and storing the collected exfoliated cells in a collection card or extracting DNA nucleic acid from a blood sample; b) adding a saliva collection card sample or an extracted DNA sample of a testee into a reaction system, and carrying out PCR amplification; c) running an amplification program; and d) carrying out electrophoretic analysis on the amplification product, and interpreting according to a peak pattern graph. According to the invention, SNP of a plurality of genes related to a testee can be synchronously detected, and simplicity, high efficiency and specificity of detection are realized. Through the analysis, reference information of the skin antioxidant capacity of the testee can be provided for the testee.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a kit and a method for detecting human skin anti-oxidation ability genotype. Background technique [0002] As global aging continues to occur, scientists are trying to gain insight into the physiological and biochemical reactions involved in human aging. The life activities of the human body require energy, which comes from the daily diet. The food enters the cells through the human body's circulatory system, and then releases energy, but a large number of free radicals are also produced while releasing energy. Free radicals are the main culprit of human aging. As we grow older, the human body will produce more free radicals, which will slowly damage the cells in the human body and lead to accelerated cell aging. Human metabolism is actually an oxidation process, and skin changes are one of the most obvious signs, so more and more people begin to pay attention to the anti-oxidation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/16C12Q2600/166C12Q2531/113C12Q2537/143C12Q2545/101
Inventor 严珂尔徐智孔咪咪余丁吴勇
Owner NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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