Kit for detecting oxidation resistance genotype of human skin and method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of antioxidant capacity and genotype, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc.
Inactive Publication Date: 2021-06-11
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
View PDF6 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0006] At present, all kinds of anti-oxidant skin care products on the market have different labels for different effects. Although a large number of tests have been carried out, there are still certain limitations.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0039](1) Selected samples: exfoliated cell samples from the oral cavity wall of subject 1 and subject 2.
[0040] (2) Sample collection
[0041] Collection type: Exfoliated cells from the inner wall of the oral cavity.
[0042] Collection method: Use the saliva collection stick to wipe back and forth on the inner wall of the oral cavity 4 times, continue to wipe back and forth on the inner wall of the oral cavity 4 times with the reverse side of the saliva collection stick, take out the saliva collection stick, and press repeatedly on the saliva sample collection card to collect the saliva inner wall cells Transfer to the saliva sample collection card, and dry the collected saliva sample collection card in a pollution-free area.
[0043] Valid sample: The area where the pink area of the saliva sample collection card changes to light pink or white is the valid saliva sample area.
[0044] Sample selection method: use Dubbo plastic puncher (1.0mm) for manual punching and sa...
Embodiment 2
[0064] (1) Selected sample: the blood sample of subject 1.
[0065] (2) Sample type: blood sample.
[0066] (3) DNA extraction: DNA extraction is performed on the blood sample using a nucleic acid extraction instrument.
[0067] (4) System configuration
[0068] The PCR reaction solution and the primers Primer Mix A and Primer Mix B were configured according to the proportion of the instruction manual. After vortex mixing, centrifuge with a centrifuge, and then use a pipette gun to distribute according to volume.
[0069] (5) Adding samples: According to the instructions, take a certain amount of extracted DNA samples with a pipette and add them to the reaction system.
[0070] (6) Amplification program
[0071] The amplification program on the PCR instrument is shown in Table 2.
[0072] (7) The amplified product is detected on the 3500DX genetic analyzer
[0073] The sample loading mixture is composed of deionized formamide and molecular weight internal standard (Size-5...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a kit for detecting oxidation resistance genotype of human skin and a methodthereof. The invention adopts multiple PCR amplification and electrophoresis methods to analyze and identify six allele polymorphism (SNP) sites in four genes (NQ01, SOD2, NFE2L2 and GPX1). The method comprises the following steps: a) collecting exfoliated cells in the oral cavity of a testee, and storing the collected exfoliated cells in a collection card or extracting DNA nucleic acid from a blood sample; b) adding a saliva collection card sample or an extracted DNA sample of a testee into a reaction system, and carrying out PCR amplification; c) running an amplification program; and d) carrying out electrophoretic analysis on the amplification product, and interpreting according to a peak pattern graph. According to the invention, SNP of a plurality of genes related to a testee can be synchronously detected, and simplicity, high efficiency and specificity of detection are realized. Through the analysis, reference information of the skin antioxidant capacity of the testee can be provided for the testee.
Description
technical field [0001] The invention relates to the field of gene detection, in particular to a kit and a method for detecting human skin anti-oxidation ability genotype. Background technique [0002] As global aging continues to occur, scientists are trying to gain insight into the physiological and biochemical reactions involved in human aging. The life activities of the human body require energy, which comes from the daily diet. The food enters the cells through the human body's circulatory system, and then releases energy, but a large number of free radicals are also produced while releasing energy. Free radicals are the main culprit of human aging. As we grow older, the human body will produce more free radicals, which will slowly damage the cells in the human body and lead to accelerated cell aging. Human metabolism is actually an oxidation process, and skin changes are one of the most obvious signs, so more and more people begin to pay attention to the anti-oxidation...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more