Kit and method for detecting human scientific temperament potential genotypes

A technology of genotype and kit, applied in the field of kits for detecting potential genotypes of human scientific temperament

Inactive Publication Date: 2021-06-08
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The impact of gene mutations caused by SNPs on humans has not yet been fully clarified, but studies have shown

Method used

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  • Kit and method for detecting human scientific temperament potential genotypes
  • Kit and method for detecting human scientific temperament potential genotypes
  • Kit and method for detecting human scientific temperament potential genotypes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Selected sample: DNA / RNA-free water.

[0049] (2) System configuration

[0050] According to the instructions, prepare the primer Primer Mix and PCR reaction solution on ice according to the ratio of the instructions to configure the reaction system. After vortexing and mixing, centrifuge with a centrifuge, and then use a pipette tip to mix and distribute.

[0051] (3) Add samples

[0052] According to the instructions, use a pipette to take the corresponding volume of DNA / RNA-free water and add it to the reaction system that has been aliquoted.

[0053] (4) Amplification program

[0054] The amplification program on the PCR instrument is shown in Table 2.

[0055] (5) The amplified product is detected on the 3500DX genetic analyzer

[0056] The sample loading mixture is composed of deionized formamide and molecular weight internal standard (Size-500) in the system {(1μL Size-500+12μL deionized formamide)×(injection number)}. Mix 9 μL of the loading mixture wit...

Embodiment 2

[0062] (1) Selected samples: exfoliated cell samples from the oral cavity wall of subject 1 and subject 2.

[0063] (2) Sample collection

[0064] Collection type: Exfoliated cells from the inner wall of the oral cavity.

[0065] Collection method: Use the saliva collection stick to wipe back and forth on the inner wall of the oral cavity 4 times, continue to wipe back and forth on the inner wall of the oral cavity 4 times with the reverse side of the saliva collection stick, take out the saliva collection stick, and press repeatedly on the saliva sample collection card to collect the saliva inner wall cells Transfer to the saliva sample collection card, and dry the collected saliva sample collection card in a pollution-free area.

[0066] Valid sample: The area where the pink area of ​​the saliva sample collection card changes to light pink or white is the valid saliva sample area.

[0067] Sample selection method: use Dubbo plastic puncher (1.0mm) for manual punching and s...

Embodiment 3

[0087] (1) Selected sample: the blood sample of subject 2.

[0088] (2) Sample type: blood sample.

[0089] (3) DNA extraction: DNA extraction is performed on the blood sample using a nucleic acid extraction instrument.

[0090] (4) System configuration

[0091] According to the instructions, prepare the primer Primer Mix and PCR reaction solution on ice according to the ratio of the instructions to configure the reaction system. After vortexing and mixing, centrifuge with a centrifuge, and then use a pipette tip to mix and distribute.

[0092] (5) Adding samples: According to the instructions, take a certain amount of extracted DNA samples with a pipette and add them to the reaction system.

[0093] (6) Amplification program

[0094] The amplification program on the PCR instrument is shown in Table 2.

[0095] (7) The amplified product is detected on the 3500DX genetic analyzer

[0096] The sample loading mixture is composed of deionized formamide and molecular weight in...

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Abstract

The invention discloses a kit and a method for detecting human scientific temperament potential genotypes. The kit and the method adopt multiple PCR amplification and electrophoresis methods to analyze and identify allele polymorphism (SNP) of five genes: NRXN1, PCDH18, TCERG1L, COX10 and CADM2. The method comprises the following steps: a) collecting oral exfoliated cells of a testee and storing the oral exfoliated cells in an acquisition card or performing DNA nucleic acid extraction on a blood sample; b) adding a saliva acquisition card sample of the testee or an extracted DNA sample into a reaction system, and performing PCR amplification; c) running an amplification program; and d) performing electrophoretic analysis on an amplification product, and performing interpretation according to a peak pattern. The kit and the method can synchronously detect the SNP of a plurality of related genes of the testee, and simplicity, high efficiency and specificity of detection are realized. Through the analysis, a reference is provided for whether the testee has scientific temperament potential or not.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a kit and method for detecting human scientific temperament potential genotype. Background technique [0002] Rockefeller once said: "Work is a stage where we can display our talents. The knowledge we have studied hard, our resilience, our determination, our adaptability and our coordination will all be on this stage. No activity other than work offers such a high degree of self-fulfillment, opportunity for self-expression, and such a strong sense of personal purpose and a reason to live. The quality of work often determines the quality of life." [0003] Ability refers to the ability to carry out various activities, which ensures that people can effectively understand the world. Ability includes various abilities that individuals must possess in cognitive activities, such as perception ability (observation ability), memory, imagination, thinking ability, attention, etc. [0004] ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686
CPCC12Q1/6888C12Q1/686C12Q2600/156C12Q2600/16C12Q2565/125C12Q2545/101C12Q2537/143
Inventor 陈屹宇徐智孔咪咪余丁吴勇
Owner NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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