Kit for detecting human fitness potential genotype and method thereof

Abstract

The invention discloses a kit for detecting human fitness potential genotypes and a method thereof. The allele polymorphism (SNP), namely CREB1, ACSL1 and MTC1, of three genes is analyzed and identified by adopting a multiple PCR amplification and electrophoresis method. The method comprises the following steps: a) collecting exfoliated cells in the oral cavity of a testee and storing the collected exfoliated cells in a collection card or performing DNA nucleic acid extraction on a blood sample; b) adding a saliva collection card sample or an extracted DNA sample of a testee into a reaction system, and carrying out PCR amplification; c) running an amplification program; and d) carrying out electrophoretic analysis on the amplification product, and interpreting according to a peak pattern graph. According to the invention, SNP of a plurality of genes related to a testee can be synchronously detected, and simplicity, high efficiency and specificity of detection are realized. Through the analysis, a reference is provided for the testee to adapt to which fitness mode.

Description

technical field

[0001] The invention relates to the field of gene detection, in particular to a kit and method for detecting human fitness potential genotypes. Background technique

[0002] On September 6, 2009, the State Council Order No. 560 "National Fitness Regulations" was officially promulgated and will come into effect on October 1. As my country's first "document" for national fitness legislation, its promulgation not only has guiding significance for promoting the development of mass sports in our country, but also provides a solid legal guarantee for the broad masses of our country to participate in sports activities.

[0003] The purpose of fitness exercise is: through orderly and harmonious activities of the body, improve the level of physical health, develop physical functions and qualities, and finally achieve the goal of preventing and curing diseases and prolonging life. However, in the development of sports in the real society, the scientific level of mass ...

Examples

Embodiment 1

[0048](1) Selected sample: DNA / RNA-free water.

[0049] (2) System configuration

[0050] According to the instructions, prepare the primer Primer Mix and PCR reaction solution on ice according to the ratio of the instructions to configure the reaction system. After vortexing and mixing, centrifuge with a centrifuge, and then use a pipette tip to mix and distribute.

[0051] (3) Add samples

[0052] According to the instructions, use a pipette to take the corresponding volume of DNA / RNA-free water and add it to the reaction system that has been aliquoted.

[0053] (4) Amplification program

[0054] The amplification program on the PCR instrument is shown in Table 2.

[0055] (5) The amplified product is detected on the 3500DX genetic analyzer

[0056] The sample loading mixture is composed of deionized formamide and molecular weight internal standard (Size-500) in the system {(1μL Size-500+12μL deionized formamide)×(injection number)}. Mix 9 μL of the loading mixture with...

Embodiment 2

[0062] (1) Selected samples: exfoliated cell samples from the oral cavity wall of subject 1 and subject 2.

[0063] (2) Sample collection

[0064] Collection type: Exfoliated cells from the inner wall of the oral cavity.

[0065] Collection method: Use the saliva collection stick to wipe back and forth on the inner wall of the oral cavity 4 times, continue to wipe back and forth on the inner wall of the oral cavity 4 times with the reverse side of the saliva collection stick, take out the saliva collection stick, and press repeatedly on the saliva sample collection card to collect the saliva inner wall cells Transfer to the saliva sample collection card, and dry the collected saliva sample collection card in a pollution-free area.

[0066] Valid sample: The area where the pink area of ​​the saliva sample collection card changes to light pink or white is the valid saliva sample area.

[0067] Sample selection method: use Dubbo plastic puncher (1.0mm) for manual punching and s...

Embodiment 3

[0087] (1) Selected sample: the blood sample of subject 2.

[0088] (2) Sample type: blood sample.

[0089] (3) DNA extraction: DNA extraction is performed on the blood sample using a nucleic acid extraction instrument.

[0090] (4) System configuration

[0091] According to the instructions, prepare the primer Primer Mix and PCR reaction solution on ice according to the ratio of the instructions to configure the reaction system. After vortexing and mixing, centrifuge with a centrifuge, and then use a pipette tip to mix and distribute.

[0092] (5) Adding samples: According to the instructions, take a certain amount of extracted DNA samples with a pipette and add them to the reaction system.

[0093] (6) Amplification program

[0094] The amplification program on the PCR instrument is shown in Table 2.

[0095] (7) The amplified product is detected on the 3500DX genetic analyzer

[0096] The sample loading mixture is composed of deionized formamide and molecular weight in...

technical field

[0001] The invention relates to the field of gene detection, in particular to a kit and method for detecting human fitness potential genotypes. Background technique

[0002] On September 6, 2009, the State Council Order No. 560 "National Fitness Regulations" was officially promulgated and will come into effect on October 1. As my country's first "document" for national fitness legislation, its promulgation not only has guiding significance for promoting the development of mass sports in our country, but also provides a solid legal guarantee for the broad masses of our country to participate in sports activities.

[0003] The purpose of fitness exercise is: through orderly and harmonious activities of the body, improve the level of physical health, develop physical functions and qualities, and finally achieve the goal of preventing and curing diseases and prolonging life. However, in the development of sports in the real society, the scientific level of mass ...

Examples

Embodiment 1

[0048](1) Selected sample: DNA / RNA-free water.

[0049] (2) System configuration

[0050] According to the instructions, prepare the primer Primer Mix and PCR reaction solution on ice according to the ratio of the instructions to configure the reaction system. After vortexing and mixing, centrifuge with a centrifuge, and then use a pipette tip to mix and distribute.

[0051] (3) Add samples

[0052] According to the instructions, use a pipette to take the corresponding volume of DNA / RNA-free water and add it to the reaction system that has been aliquoted.

[0053] (4) Amplification program

[0054] The amplification program on the PCR instrument is shown in Table 2.

[0055] (5) The amplified product is detected on the 3500DX genetic analyzer

[0056] The sample loading mixture is composed of deionized formamide and molecular weight internal standard (Size-500) in the system {(1μL Size-500+12μL deionized formamide)×(injection number)}. Mix 9 μL of the loading mixture with...

Embodiment 2

[0062] (1) Selected samples: exfoliated cell samples from the oral cavity wall of subject 1 and subject 2.

[0063] (2) Sample collection

[0064] Collection type: Exfoliated cells from the inner wall of the oral cavity.

[0065] Collection method: Use the saliva collection stick to wipe back and forth on the inner wall of the oral cavity 4 times, continue to wipe back and forth on the inner wall of the oral cavity 4 times with the reverse side of the saliva collection stick, take out the saliva collection stick, and press repeatedly on the saliva sample collection card to collect the saliva inner wall cells Transfer to the saliva sample collection card, and dry the collected saliva sample collection card in a pollution-free area.

[0066] Valid sample: The area where the pink area of ​​the saliva sample collection card changes to light pink or white is the valid saliva sample area.

[0067] Sample selection method: use Dubbo plastic puncher (1.0mm) for manual punching and s...

Embodiment 3

[0087] (1) Selected sample: the blood sample of subject 2.

[0088] (2) Sample type: blood sample.

[0089] (3) DNA extraction: DNA extraction is performed on the blood sample using a nucleic acid extraction instrument.

[0090] (4) System configuration

[0091] According to the instructions, prepare the primer Primer Mix and PCR reaction solution on ice according to the ratio of the instructions to configure the reaction system. After vortexing and mixing, centrifuge with a centrifuge, and then use a pipette tip to mix and distribute.

[0092] (5) Adding samples: According to the instructions, take a certain amount of extracted DNA samples with a pipette and add them to the reaction system.

[0093] (6) Amplification program

[0094] The amplification program on the PCR instrument is shown in Table 2.

[0095] (7) The amplified product is detected on the 3500DX genetic analyzer

[0096] The sample loading mixture is composed of deionized formamide and molecular weight in...

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