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Antibodies to detect novel coronavirus

A coronavirus and antibody technology, applied in the fields of molecular biology and cellular immunology, can solve the problems of missed diagnosis, false positive and false negative, and large impact

Active Publication Date: 2022-02-11
JIANGSU PROVINCIAL CENT FOR DISEASE CONTROL & PREVENTION PUBLIC HEALTH RES INST OF JIANGSU PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although nucleic acid diagnosis is fast, it is greatly affected by the quality of sampling, and there are false positives and false negatives, which affect the implementation of prevention and control measures
Some asymptomatic infected persons are also negative in the nucleic acid test in the late stage of the disease, and it is easy to miss the diagnosis based on the nucleic acid test alone

Method used

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  • Antibodies to detect novel coronavirus
  • Antibodies to detect novel coronavirus
  • Antibodies to detect novel coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1 Antibody Screening

[0087] 1. Expression of recombinant SARS-CoV2 nucleoprotein (NP)

[0088] 1.1 Main reagents

[0089] The SARS-CoV2 NP gene sequence (GenBank serial number: MT066176.1) and related primers were synthesized and sequenced by General Biosystems (Anhui) Co., Ltd.; E.coli DH5α, BL21(DE3) competent cells were purchased from General Biosystems (Anhui) Co., Ltd.; BamHI and NotI endonucleases were purchased from New England Biolabs (NEB); EXTaq enzyme was purchased from TaKaRa; HRP-labeled anti-human Fc antibody was purchased from Sigma; other chemical reagents were domestic analytical reagents ;The serum of patients infected with 2019-nCoV is collected and preserved by our center.

[0090] 1.2 Prokaryotic expression plasmid construction

[0091] The primers for prokaryotic expression of NP gene were designed, the upstream primer had a BamHI restriction site, and the downstream primer had a NotⅠ restriction site. The primer sequences are: Cov2-N...

Embodiment 2

[0142] Example 2 Expression of whole antibody and related functional verification

[0143] From the obtained 159 antibodies, 16 antibodies were finally selected for the expression of the whole antibody and related functional verification, and these 16 antibodies were named JS01-JS16.

[0144] Among them, the JS05 antibody sequence is as follows:

[0145] The amino acid sequence of the heavy chain variable region CDR1 is shown in SEQ ID NO.1;

[0146] The amino acid sequence of the heavy chain variable region CDR2 is shown in SEQ ID NO.2;

[0147] The amino acid sequence of the heavy chain variable region CDR3 is shown in SEQ ID NO.3;

[0148] The amino acid sequence of the light chain variable region CDR1 is shown in SEQ ID NO.5;

[0149] The amino acid sequence of the light chain variable region CDR2 is shown in SEQ ID NO.6;

[0150] The amino acid sequence of the light chain variable region CDR3 is shown in SEQ ID NO.7;

[0151] The amino acid sequence of the heavy chai...

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Abstract

The invention discloses an antibody for detecting novel coronavirus, the antibody has a heavy chain variable region shown in SEQ ID NO.4 and a light chain variable region shown in SEQ ID NO.8. The antibody of the present invention can be used for the effective detection of novel coronavirus contained in serum by ELISA method.

Description

technical field [0001] The invention belongs to the fields of cellular immunology and molecular biology, and relates to antibodies for detecting novel coronaviruses. Background technique [0002] The International Committee on Taxonomy of Viruses named the new coronavirus as SARS-CoV-2, and the World Health Organization named the pneumonia caused by this virus as COVID-19. The virus is highly contagious and spreads widely. The virus can quickly adapt to the human environment, and has the ability to spread during the incubation period after infection. At the same time, some asymptomatic infections have been reported, and viral nucleic acids have even been detected in various animals. These factors make the prevention and control of the virus very complicated, and there are currently no effective treatments and vaccines on the market. SARS-CoV-2 belongs to the genus Coronaviridae and is a single-stranded positive-sense RNA virus with a size of about 30kb. The similarity with...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C12N15/13G01N33/569G01N33/68
CPCC07K16/10G01N33/56983G01N33/6854C07K2317/565C07K2317/92G01N2333/165
Inventor 张黎郑滨洋高行素孟繁岳崔仑标朱凤才
Owner JIANGSU PROVINCIAL CENT FOR DISEASE CONTROL & PREVENTION PUBLIC HEALTH RES INST OF JIANGSU PROVINCE