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Preparation method and application of hydrochloric ether anaerobic degradation microbial inoculum

A technology for chlorinated hydrocarbons and degrading bacteria is applied in the field of biological treatment of groundwater pollution, which can solve the problems of incomplete restoration of chlorinated hydrocarbons, incomplete conversion of chlorinated hydrocarbons, incomplete dechlorination, etc.

Inactive Publication Date: 2021-06-25
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The PCR results of soil and groundwater samples from chlorinated hydrocarbon-contaminated sites showed that there were dechlorinating bacteria, but due to the low function and abundance of dechlorinating bacteria, there were incomplete remediation of chlorinated hydrocarbons in the groundwater of the site, including incomplete dechlorination ( With DCE and VC as the main products) and incomplete conversion of chlorinated hydrocarbons

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  • Preparation method and application of hydrochloric ether anaerobic degradation microbial inoculum
  • Preparation method and application of hydrochloric ether anaerobic degradation microbial inoculum
  • Preparation method and application of hydrochloric ether anaerobic degradation microbial inoculum

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preparation example Construction

[0037] The invention relates to a preparation method of an anaerobic degradation bacterial agent for chlorinated hydrocarbons, comprising:

[0038] a) Get culture medium:

[0039] The culture medium is a mixture of basic mineral and inorganic salt culture medium and additionally added trace element solution, vitamin solution and trichlorethylene, wherein the concentration of trichlorethylene is 0.9mM-1.3mM, and the culture medium is passed through an anaerobic Oxygen and aseptic processing;

[0040] b) Inoculate the river bottom mud polluted by chlorinated hydrocarbons in the culture medium in the dark until trichlorethylene is converted into ethylene, then take out part of the culture and inoculate it in the new medium, and repeat the operation until it is replaced Culture medium totaled 8-14 times.

[0041] The invention focuses on the whole biotransformation process of chlorinated hydrocarbons, further converts harmful products into non-toxic products, and broadens the ra...

Embodiment 1

[0077] This embodiment provides a kind of preparation method of the chlorinated hydrocarbon anaerobic degradation bacterial agent of complete dechlorination, and preparation method comprises the following steps:

[0078] (1) Preparation of anaerobic mineral inorganic salt medium: prepare anaerobic mineral inorganic salt medium in a 2000mL three-necked round bottom flask, pump 60mL of medium into 120mL anaerobic serum bottles with a 60mL syringe;

[0079] (2) Anaerobic treatment: to (1) the deoxygenation operation of the anaerobic mineral inorganic salt culture medium in the three-necked round-bottomed flask, the specific steps include blocking the mouth in the middle of the three-necked round-bottomed flask with a rubber stopper, and passing into the lower mouth from the right side Nitrogen gas at a high flow rate (pressure not exceeding 2 bar) was sent to the bottom of the round-bottomed flask, and the whole nitrogen gas flow process was maintained for 45 minutes. Before disp...

Embodiment 2

[0092] The application of the above-mentioned chlorinated hydrocarbon anaerobic degradation bacterial agent in the remediation of groundwater polluted by TCE, DCE, VC and DCA. The repairing conditions of the bacteria agent are: the concentration of chlorinated hydrocarbon is about 1mM, the inoculation amount is 2% or 10%, and 10mM sodium lactate is added as electron donor and carbon source.

[0093] Such as figure 1 As shown, when the initial concentration of TCE was 1mM, TCE was completely degraded in 0-18 days, and DCE and VC were detected to be continuously generated and accumulated at the same time, and the generation of ethylene was detected at 13 days, and VC no longer increased at 17 days, and further conversion For ethylene, by 30 days, TCE was converted to VC via DCE, and finally completely converted to ethylene, achieving a 100% dechlorination rate of TCE.

[0094] Such as figure 2 As shown, the concentration of DCE decreased from 1mM to 0 within 22 days, accompan...

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Abstract

The invention relates to the field of underground water pollution treatment through a biological method, in particular to a preparation method and application of a hydrochloric ether anaerobic degradation microbial inoculum. The preparation method comprises the following steps of: a) acquiring a culture medium, wherein the culture medium is a mixture of a basic mineral inorganic salt culture medium and additionally added trace element liquid, vitamin liquid and trichloroethylene, the concentration of the trichloroethylene is 0.9 mM to 1.3 mM, and the culture medium is subjected to anaerobic and sterile treatment; and b) inoculating river sediment containing hydrochloric ether into the culture medium, culturing in a dark place until trichloroethylene is converted into ethylene, taking out part of culture, inoculating into a new culture medium, and repeating the operation until the culture medium is changed for 8-14 times in total, thereby acquiring the hydrochloric ether anaerobic degradation microbial inoculum. The microbial inoculum prepared by the invention not only considers the toxicity control of products in the whole process of bioconversion of hydrochloric ether, but further considers the range diversification of hydrochloric ether types repaired by using a hydrochloric ether repair microbial inoculum.

Description

technical field [0001] The invention relates to the field of biological treatment of groundwater pollution, in particular to a preparation method and application of a chlorinated hydrocarbon anaerobic degradation bacterial agent. Background technique [0002] As important organic solvents and product intermediates, chlorinated hydrocarbons are widely used in metal processing, electroplating, dry cleaning and other industries. They enter the environment through production, irregular use and accidental leakage, and then enter the groundwater to cause pollution. Tetrachlorethylene (PCE), trichlorethylene (TCE), dichlorethylene (DCE), vinyl chloride (VC) and 1,2-dichloroethane (DCA) are the most common pollutants in groundwater in my country and other countries in the world. substance. These chlorinated hydrocarbons all have the "three causes" effect, which can cause nerve, kidney and liver damage, and have been included in the list of priority pollutants in water by various cou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/30C12N1/20C02F3/34C12R1/01C02F101/36C02F103/06
CPCC12N1/30C12N1/20C02F3/34C02F2101/36C02F2103/06
Inventor 张芳曹利锋徐文馨李广贺张旭侯德义
Owner TSINGHUA UNIV
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