Novel urea 6,7-dihydro-4h-thiazolo[5,4-c]pyridines active against the hepatitis b virus (HBV)
A C1-C4-, C1-C6-technology, applied in the field of new antiviral agents, can solve problems such as poor efficacy, difficult synthesis, and mutagenicity
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Embodiment 1
[0454] 2-Amino-N-(3-chloro-4-fluorophenyl)-4H,5H,6H,7H-[1,3]thiazolo[5,4-c]pyridine-5-carboxamide
[0455]
[0456] Rt (Method A) 2.95mins, m / z 327 / 329[M+H]+
[0457] 1 H NMR (400MHz, DMSO-d6) δ8.79 (s, 1H), 7.75 (dd, J = 6.9, 2.6Hz, 1H), 7.42 (ddd, J = 9.1, 4.4, 2.7Hz, 1H), 7.29 ( t, J = 9.1 Hz, 1H), 6.82 (s, 2H), 4.47-4.40 (m, 2H), 3.75-3.67 (m, 2H), 2.56-2.51 (m, 2H).
Embodiment 2
[0459]N-(3-chloro-4-fluorophenyl)-2-{[(1r,3r)-3-hydroxycyclobutyl]amino}-4H,5H,6H,7H-[1,3]thiazolo[ 5,4-c]pyridine-5-carboxamide
[0460]
[0461] Step 1: To tert-butyl 2-(((1r,3r)-3-hydroxycyclobutyl)amino)-6,7-dihydrothiazolo[5,4-c]pyridine-5(4H)-carboxylate (1.77 g, 5.44 mmol) was added 4M HCl in dioxane (15 mL, 60 mmol). The mixture was stirred at room temperature for 4 hours, then concentrated in vacuo. The residue was washed with toluene (twice) and CH 2 Cl 2 Stripping gave 1.54 g of a white solid which was used without further purification.
[0462] Step 2: To (1r,3r)-3-((4,5,6,7-tetrahydrothiazolo[5,4-c]pyridin-2-yl)amino)cyclobutan-1-ol hydrochloride (50 mg, 0.191 mmol) and DIPEA (0.167 mL, 0.955 mmol) in anhydrous N,N-dimethylformamide (2 mL) was added 2-chloro-1-fluoro-4-isocyanatobenzene ( 0.024 mL, 0.191 mmol). The mixture was stirred at r.t. for 30 minutes, then water was added. The product was extracted with EtOAc (2x4mL), and the combined organic ext...
Embodiment 3
[0466] N-(3-chloro-4-fluorophenyl)-2-{[1-(hydroxymethyl)cyclobutyl]amino}-4H,5H,6H,7H-[1,3]thiazolo[5, 4-c]pyridine-5-carboxamide
[0467]
[0468] Rt (Method A) 3.15mins, m / z 411 / 413[M+H]+
[0469] 1 H NMR (400MHz, DMSO-d6) δ8.81(s, 1H), 7.75(dd, J=6.9, 2.6Hz, 1H), 7.57(s, 1H), 7.45-7.39(m, 1H), 7.29( t,J=9.1Hz,1H),4.96(t,J=5.6Hz,1H),4.46-4.41(m,2H),3.75-3.67(m,2H),3.62(d,J=5.6Hz,2H ), 2.55-2.51(m,2H), 2.14-2.04(m,4H), 1.89-1.75(m,1H), 1.75-1.65(m,1H).
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