Fano resonance-based surface-enhanced Raman sensing substrate and production method thereof
A surface-enhanced Raman and Fano resonance technology, applied in the field of optics, can solve the problems of complex preparation, high cost and difficult application.
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Embodiment 1
[0042] This embodiment provides a nanosphere-polyethylene glycol-gold film structure surface-enhanced Raman sensing substrate based on Fano resonance, and its structure is as follows figure 1As shown, the surface-enhanced Raman sensing substrate is prepared on a silicon substrate, specifically including: a gold film and nanospheres, wherein the nanospheres are silica nanospheres modified with Avidin (avidin); The surface of the gold film is chemically modified with Biotin-PEG-SH (mercapto-polyethylene glycol-biotin) chemical reagent, and the mercapto group (SH) is strongly chemically connected with the gold film, and PEG (polyethylene glycol) Used to control the length through different molecular weights, Biotin (biotin) and Avidin (avidin) are strongly chemically linked, such as figure 2 shown; and, the surface of the silica nanospheres is coated with a gold-plated layer.
[0043] In this embodiment, in the surface-enhanced Raman sensing substrate structure, the thickness o...
Embodiment 2
[0067] This embodiment provides a nanosphere-polyethylene glycol-gold film structure surface-enhanced Raman sensing substrate based on Fano resonance, and its structure is as follows figure 1 As shown, the surface-enhanced Raman sensing substrate is prepared on a silicon substrate, specifically including: a gold film and nanospheres, wherein the nanospheres are gold nanospheres, and SH-Avidin (mercapto-avidin) is used Carry out chemical modification, make it have Avidin (avidin) modification on the surface; The surface of the gold film adopts Biotin-PEG-SH (mercapto-polyethylene glycol-biotin) chemical reagent to carry out chemical modification, mercapto (SH) and all The gold film is strongly chemically connected, PEG (polyethylene glycol) is used to control the length through different molecular weights, and Biotin (biotin) is strongly chemically connected to Avidin (avidin), such as figure 2 shown.
[0068] Compared with Example 1, this embodiment differs in that: when gol...
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