Broad-spectrum anti-variant new coronavirus IgY, composite antibody, preparation method and combined preparation

A compound antigen and virus technology, applied in antiviral agents, viruses/bacteriophages, chemical instruments and methods, etc., can solve problems such as weakening of antibodies, discounting of drug efficacy, loss of neutralization effect, etc.

Pending Publication Date: 2021-07-09
SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the face of this catastrophe, the global medical community has urgently launched the research and development of vaccines and therapeutic drugs; however, a large number of studies and clinical experience have shown that the degree of glycosylation of the new coronavirus is unusual, and the study found that the glycosylation site of HIV is the same as that of influenza. 3-6 times that of the virus (this is one of the main reasons why HIV vaccine development has been delayed), and the glycosylation site of the new coronavirus is more than 2 times that of the HIV virus, which means that the new coronavirus is very easy to mutate ; Most of the new coronaviruses currently circulating in Europe and the United States are variants of previously circulated viruses, and the mutated viruses are much more contagious
The more serious threat to human beings is that, if the virus mutates, the antibodies produced by conventional methods will weaken or even lose their neutralizing effect; at the same time, the new coronavirus also has an immune escape mechanism, which will further reduce the efficacy of the drug
These characteristics of the new coronavirus pose great challenges to the development of therapeutic drugs

Method used

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  • Broad-spectrum anti-variant new coronavirus IgY, composite antibody, preparation method and combined preparation
  • Broad-spectrum anti-variant new coronavirus IgY, composite antibody, preparation method and combined preparation
  • Broad-spectrum anti-variant new coronavirus IgY, composite antibody, preparation method and combined preparation

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preparation example Construction

[0222] 2. Preparation of anti-mutant new coronavirus IgY

[0223] Considering the high mutational nature of the new coronavirus, even antibodies that can recognize conserved epitopes cannot recognize all mutant new coronaviruses. In particular, the mutation sites of the N501Y recently discovered in Europe and the dE484K recently discovered in South Africa are the key epitopes for the binding of the new coronavirus to human cells, which will greatly affect the actual effect of the previously developed antibody drugs. influences. Therefore, in order to completely solve the problem that the new coronavirus is easy to mutate and affect the curative effect, it is necessary to develop several corresponding anti-mutation new coronavirus IgYs for the mutations of key antigenic sites that are currently appearing. The present invention uses G614, N510K, and dE484K as examples to illustrate. Other new coronaviruses with key epitope mutations that will appear in the future can refer to t...

Embodiment 1

[0368] Example 1: Determination of the titer of broad-spectrum anti-mutant new coronavirus compound IgY neutralizing mutant new coronavirus by neutralization test

[0369] 1. Test material:

[0370] (1) Anti-broad-spectrum anti-mutation new coronavirus compound IgY: protein content 40mg / ml No. 1 specimen 1

[0371] (2) HRP enzyme-labeled rabbit anti-chicken IgG

[0372] (3) Negative antibody (IgY antibody of unimmunized eggs): 1 part of No. 2 specimen with protein content of 60mg / ml

[0373] (4) Positive control: 1 convalescent serum from a patient infected with the new coronavirus, specimen No. 3

[0374] (5) Negative control: 1 baby umbilical cord serum, specimen No. 4

[0375] (6) Viruses:

[0376] a. SARS-CoV-2 virus isolate I: Isolation and identification from throat test specimens of patients infected with SARS-CoV-2 virus.

[0377] b. SARS-CoV-2 virus isolate II: isolated and identified from serum samples of patients infected with SARS-CoV-2 virus.

[0378] (7) Ce...

Embodiment 2

[0392] Example 2: Production of broad-spectrum anti-mutation new coronavirus compound IgY atomizer for central air-conditioning, adding this atomizer to the water of central air-conditioning humidifier in a certain proportion, fully stirring evenly, and then mixing with air-conditioning after atomization The fresh air is mixed and sent into the room. , to achieve the effect of killing the new coronavirus that may exist in the indoor air.

[0393] formula:

[0394]

[0395] Process:

[0396] (1) Sterilize and sterilize the formula amount of pharmaceutical grade glycerin with ultraviolet light for 24 hours, and aseptically seal it for later use;

[0397] (2) Heat distilled water according to the formula to 90°C, keep it for 10 minutes, then cool to 60°C, then add pharmaceutical grade glycerin, stir at low speed for 60 minutes until fully dissolved; then, cool to room temperature to form solution A;

[0398] (3) While stirring, add the broad-spectrum anti-mutant new coronav...

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Abstract

The invention provides a broad-spectrum anti-variant new coronavirus IgY, a composite antibody, a preparation method and a combined preparation. The invention provides a method for preparing a broad-spectrum anti-mutated new coronavirus IgY antibody, an anti-mutated new coronavirus IgY antibody and a combined antibody. The IgY, the composite IgY and the combined antibody can be combined with antigenic epitopes with strong conservative property to achieve a broad-spectrum neutralization effect, and the problem that the new coronavirus is easy to mutate and immune escape is solved. The invention also develops a series of preparations containing the broad-spectrum anti-variant new coronavirus composite IgY. The invention also provides a central air-conditioning atomization disinfectant and an external package spray disinfectant prepared from the IgY and the composite IgY, and an atomization inhalation atomization agent, a spray, a mouth spray, a nasal spray, a nose drop, an eye drop, an air disinfectant, a hand sanitizer, powder, tablets, buccal tablets, oral liquid and capsules. The compound is applied to medicines, disinfection products, health care products and medical instruments for preventing and treating new coronavirus infection.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a broad-spectrum anti-mutated new coronavirus IgY and composite antibody, a preparation method and a combined preparation. Background technique [0002] The SARS-CoV-2 coronavirus is a new type of coronavirus that has never been found in humans before. So far, the number of confirmed cases worldwide has reached 100 million, and the death toll has exceeded 2 million. In the face of this catastrophe, the global medical community has urgently launched the research and development of vaccines and therapeutic drugs; however, a large number of studies and clinical experience have shown that the degree of glycosylation of the new coronavirus is unusual, and the study found that the glycosylation site of HIV is the same as that of influenza. 3-6 times that of the virus (this is one of the main reasons why HIV vaccine development has been delayed), and the glycosylation site of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/02C07K14/165A61K39/42A61P31/14
CPCC07K16/10C07K16/02C07K14/005A61P31/14C12N2770/20022A61K2039/505
Inventor 包晟杨荣鉴龚芹杨得山
Owner SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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