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DNA aptamers specific of adenovirus types

An adenovirus-specific technology, applied in the field of specific aptamers, can solve the problems of unpublished analysis of adenoviruses, and achieve the effect of lower cost

Pending Publication Date: 2021-08-06
LUXEMBOURG INST OF SCI & TECH LIST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The documents do not disclose any tools for the analysis of adenoviruses

Method used

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  • DNA aptamers specific of adenovirus types
  • DNA aptamers specific of adenovirus types
  • DNA aptamers specific of adenovirus types

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Embodiment Construction

[0035] Production and purification of virus stock

[0036]HAdV-2 (human adenovirus type 2) was chosen for aptamer selection. HAdV-2 was obtained from the Health Protection Agency culture collection (NCPV #213) and propagated on the human embryonic kidney cell line 293A (Invitrogen). Cells were grown in Dulbecco's Modified Eagle Medium (D-MEM) containing high glucose concentrations and Glutamax (Life Technologies), supplemented with 1% non-essential amino acids (Life Technologies) and 5% non-heat-inactivated fetal bovine serum (Life Technologies). HAdV-2 stocks were prepared from infected 293A monolayers by freeze-thaw lysis, followed by centrifugation (1200 rpm, 10 min, 4°C) to remove cell debris. The virus suspension was then purified by cesium chloride equilibrium density gradient centrifugation. Purified AdV bands were collected using a syringe and injected directly into a float-a-lyser dialysis unit (Spectrum Labs, USA) with a molecular weight cut-off of 100 KDa. The d...

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Abstract

The invention is directed to a single stranded nucleic acid aptamer able to specifically bind to at least one Adenovirus type, said aptamer comprising or consisting of one of sequences SEQ ID NO:1 to 3, or variants thereof having at least 50% sequence identity. The invention is also directed to a composition comprising said aptamer. The invention is directed to use of said aptamer, for detecting, capturing, concentrating and / or quantifying at least one Adenovirus type. The invention is directed to in vitro methods for capturing, detecting and / or quantifying at least one Adenovirus type. The invention is also about a kit for detecting, quantifying, capturing and / or concentrating at least one Adenovirus type.

Description

technical field [0001] The present invention relates to specific aptamers for one or more adenovirus types and their use. Background technique [0002] Enteroviruses (enteroviruses, noroviruses, adenoviruses, and hepatitis A and E viruses, among others) are present in high concentrations in the faeces of infected individuals and can subsequently be transmitted in aquatic environments by way of wastewater effluent. These viruses are mainly transmitted from person to person, or through contaminated food and drinking water, and bathing or recreational activities. Among these viruses, human adenovirus (HAdV) is capable of causing many diseases including gastroenteritis, conjunctivitis, myocarditis and pneumonia. Geographically, these viruses are distributed throughout the world and are commonly found in various water sources, including river, sea or groundwater. They are generally more abundant than other enteroviruses (e.g., enteroviruses or noroviruses) (Lee and Kim, 2008; V...

Claims

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Application Information

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IPC IPC(8): C12N7/02C12N15/113C12N15/115G01N33/53
CPCC12N15/115C12N2310/16G01N33/56983G01N2333/075G01N33/5308
Inventor L.奥戈尔扎利S.朱丹C.穆洛兰德
Owner LUXEMBOURG INST OF SCI & TECH LIST
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