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Composite probiotic preparation capable of relieving non-alcoholic fatty liver, preparation method and application thereof

A compound probiotic, non-alcoholic technology, applied in the field of microorganisms, can solve the problems of unclear treatment effect and mechanism of NAFLD, and achieve the effect of reducing the swelling degree, number and weight of liver cells.

Pending Publication Date: 2021-08-17
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of probiotic preparations in NAFLD is rare, and the therapeutic effect and mechanism of NAFLD are not clear

Method used

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  • Composite probiotic preparation capable of relieving non-alcoholic fatty liver, preparation method and application thereof
  • Composite probiotic preparation capable of relieving non-alcoholic fatty liver, preparation method and application thereof
  • Composite probiotic preparation capable of relieving non-alcoholic fatty liver, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Preparation of Bifidobacterium animalis subsp. lactisBLa80 freeze-dried powder

[0044] Inoculate Bifidobacterium animalis subsp. lactis BLa80 into the culture medium according to the inoculum amount accounting for 3% of the total mass of the culture medium, and cultivate it at 37°C for 18 hours to obtain a culture medium; centrifuge the culture medium to obtain bacteria ; Resuspend the trehalose lyoprotectant with a trehalose concentration of 100g / L (the mass ratio of the lyoprotectant to the bacteria is 2:1) to obtain a resuspension; vacuum freeze the resuspension Freeze-drying is carried out to obtain Bifidobacterium animalis subsp. lactis BLa80 freeze-dried powder.

Embodiment 2

[0045] Embodiment 2: Preparation of Lactobacillus rhamnosus LRa05 freeze-dried powder

[0046] Lactobacillus rhamnosus LRa05 was inoculated into the medium according to the inoculum amount accounting for 3% of the total mass of the medium, and cultured at 37°C for 18 hours to obtain a culture medium; the culture medium was centrifuged to obtain the cells; the cells were used The trehalose lyoprotectant with a trehalose concentration of 100g / L was resuspended (the mass ratio of the lyoprotectant to the bacteria was 2:1) to obtain a resuspension; the resuspension was lyophilized by vacuum freezing, The freeze-dried powder of Lactobacillus rhamnosusLRa05 was obtained.

Embodiment 3

[0047] Embodiment 3: Preparation of Lactobacillus plantarum Lp90 freeze-dried powder

[0048] Lactobacillus plantarum Lp90 was inoculated into the culture medium according to the inoculum amount accounting for 3% of the total mass of the culture medium, and cultured at 37°C for 18 hours to obtain a culture solution; the culture solution was centrifuged to obtain bacteria; the bacteria were treated with trehalose Trehalose lyoprotectant with a concentration of 100g / L was resuspended (the mass ratio of lyoprotectant to bacteria was 2:1) to obtain a resuspension; the resuspension was lyophilized by vacuum freezing to obtain a plant Lactobacillus plantarum Lp90 freeze-dried powder.

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Abstract

The invention discloses a compound microbial preparation as well as a preparation method and application thereof, and relates to the technical field of microorganisms. The compound probiotic preparation is prepared from bifidobacterium animalis subsp. Lactis BLa80, lactobacillus rhamnosus LRa05 and lactobacillus plantarum Lp90 The preparation method comprises the following steps: respectively preparing freeze-dried powders of the bifidobacterium animalis subsp. Lactis BLa80, lactobacillus rhamnosus LRa05 and lactobacillus plantarum Lp90, and uniformly mixing the three freeze-dried powder in proportion to prepare the compound probiotics. The compound probiotics provided by the invention can prevent and / or treat the non-alcoholic fatty liver disease, and has a great application prospect in preparation of products (such as solid beverages) for preventing and / or treating the non-alcoholic fatty liver disease.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a compound probiotic preparation capable of alleviating non-alcoholic fatty liver, a preparation method and an application thereof. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) refers to a clinicopathological syndrome characterized by diffuse bullous steatosis in hepatic cells, excluding alcohol and other definite liver damage factors, depending on whether the lesion is accompanied by inflammation and fibrosis into simple fatty liver, NAFLD and related cirrhosis. At present, NAFLD is considered to be the hepatic manifestation of metabolic syndrome, and its pathogenesis has not been fully elucidated. Recent studies have found that its occurrence is closely related to changes in intestinal flora, and intestinal flora disorder and intestinal wall permeability increase through immune response It plays an important role in the occurrence and developm...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23L2/52A61K35/745A61K35/747A61P1/16C12R1/01C12R1/225C12R1/25
CPCC12N1/20A23L33/135A23L2/52A61K35/745A61K35/747A61P1/16A23V2002/00A23V2400/175A23V2400/169A23V2400/515A23V2200/30Y02A50/30
Inventor 方曙光盖忠辉朱建国吴明科王桂龙吴银琴
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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