Embryo culture method for obtaining distant filial generation by using singular source tetraploid Lilium spp as male parent

A technology of distant hybridization and tetraploid, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effect of promoting the germination of immature embryos and shortening the germination time of immature embryos

Active Publication Date: 2021-08-27
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on hybrid offspring obtained from 'Honesty' as the male parent

Method used

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  • Embryo culture method for obtaining distant filial generation by using singular source tetraploid Lilium spp as male parent
  • Embryo culture method for obtaining distant filial generation by using singular source tetraploid Lilium spp as male parent
  • Embryo culture method for obtaining distant filial generation by using singular source tetraploid Lilium spp as male parent

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Experimental program
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Embodiment 1

[0047] (1) Preparation of culture medium

[0048] 1) Induction medium: 1 / 2MS medium + 6-BA 0.1mg / L + NAA 0.05mg / L + sucrose 30g / L + hydrolyzed casein 500mg / L, pH 5.8.

[0049] 2) Rooting medium: 1 / 2MS medium + NAA 0.2mg / L + sucrose 30g / L + activated carbon 0.8g / L + agar 7g / L, pH 5.8.

[0050] 3) Proliferation medium: MS medium + 6-BA 0.05 mg / L + NAA 0.10 mg / L + sucrose 30 g / L + agar 7 g / L, pH 5.8.

[0051] 4) Spheroidizing medium: MS medium + sucrose 40g / L + activated carbon 0.8g / L + agar 7g / L, pH 5.8.

[0052]Taking the preparation of 1L induction medium as an example, the specific preparation process is: Weigh the parts of 1 / 2MS medium required for 1L medium to dissolve and mix in sequence; then weigh NAA 0.05 mg, 6-BA0.1mg, hydrolyzed casein 500mg, and sucrose 30g were added to the MS solution to dissolve in turn, constant volume, and the pH value was adjusted to 5.8; divided into Erlenmeyer flasks, and extinguished at a high temperature of 121°C and a high pressure of 0....

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Abstract

The invention discloses an embryo culture method for obtaining distant filial generation by using singular tetraploid Lilium spp as a male parent. The embryo culture method for obtaining the distant filial generation by using the singular tetraploid Lilium spp as the male parent comprises the steps of hybridizing singular tetraploid Lilium spp 'Honey' as the male parent with LA-line triploid and tetraploid or A-line diploid and tetraploid Lilium spp, and putting a hybridized ovule explant into a liquid induction culture medium for dark culture to obtain an immature embryo germination test-tube plantlet; transferring the obtained immature embryo germinated test-tube plantlet to a solid rooting culture medium for illumination culture to promote rooting of the test-tube plantlet; inoculating the rooted test-tube plantlet into a proliferation culture medium for illumination culture to obtain a test-tube plantlet proliferated by each filial generation; and inoculating the test-tube plantlet into a balling culture medium for dark culture to promote the filial generation to develop into scale balls. According to the embryo culture method for obtaining the distant filial generation by using the singular tetraploid Lilium spp as the male parent, generation embryo culture is carried out by infiltrating ovules in a liquid culture medium, the problem that hypoxia is easily caused by soaking in the liquid culture medium is solved, meanwhile, culture medium formulas in different stages of LA-line and A-line Lilium spp filial generation embryo culture are optimized, and the germination rate and survival rate of immature embryos are increased.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to a method for culturing embryos of lily distant hybrids. Background technique [0002] Lily (Lilium spp), a perennial plant belonging to the family Liliaceae, is a famous ornamental plant in the world and an important native medicinal and edible plant in my country. It is the development trend of the lily industry to breed high value-added lily varieties with both food and appreciation, and the germination time of lily hybrid offspring is longer. Generally, it takes 2-3 years from sowing to flowering. Some seeds are easy to rot during germination, not only The seed germination rate is reduced, and the seedling germination is irregular, and the breeding cycle is long, which seriously restricts the development of lily breeding. In addition, in the process of lily hybridization and mating, although some interspecific hybrid combinations are compatible, due to the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/008A01H4/001
Inventor 陈敏敏张永春蔡友铭杨柳燕聂功平
Owner SHANGHAI ACAD OF AGRI SCI
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