Biological material for repairing bladder tissue and preparation method thereof

Abstract

The invention provides a preparation method of a biological material for repairing bladder tissue, which comprises the following steps: selecting gelatin, and carrying out modification treatment to obtain methacrylic acid acylated hydrogel; selecting hyaluronic acid, and carrying out modification treatment to obtain methacrylic acid acylated hyaluronic acid; selecting and preparing a bladder acellular matrix, and performing modification treatment to obtain a methacrylic acid acylated bladder acellular matrix; and mixing the three materials in proportion and promoting in-situ cross-linking reaction to obtain the biological material. According to the biological material obtained by the invention, most of the components are derived from the bladder, biological active factors of the bladder are reserved, and the biological material has relatively high biological activity and good biocompatibility and can promote regeneration of bladder urinary tract epithelial cells, bladder smooth muscle cells, Cajal interstitial cells and human umbilical vein endothelial cells; and compared with the traditional biological material, the mechanical property is more excellent.

Description

technical field [0001] The invention relates to the technical fields of preparation of biomedical materials and tissue engineering, in particular to a composite biomaterial for bladder tissue repair and a preparation method thereof. Background technique [0002] The basement membrane is an important extracellular matrix present in all epithelial and endothelial tissues that maintains tissue integrity and acts as a barrier between different cells and molecules, separating different tissue types, and has mechanical Signal transmission and other functions. The main components of the basement membrane are laminin, type IV collagen, heparan sulfate proteoglycan, and various growth factors, cytokines, chemokines, and proteases. BME / matrigel, which is widely used at present, is a basement membrane extract isolated from epithelial tumors, and its composition is similar to that of the real basement membrane. However, BME / matrigel has certain disadvantages: 1) Since BME / matrigel is ...

AI Technical Summary

Problems solved by technology

However, BME / matrigel has certain disadvantages: 1) Since BME / matrigel is extracted from tumor cells, and the tumor size in animal and tissue preparations is different, there will be differences between batches; 2) directly into When gluing, there are still problems such as insufficient strength, pore size, hardness and stretchability cannot be adjusted.

However, there are still some problems in the BAM prepared by the prior art: 1) the regeneration of smooth muscle cells is not good. (Triton X-100, sodium deoxycholate and sodium lauryl sulfate, etc.) are improperly used, resulting in a greater impact on biologically active factors in the extracellular matrix; The effect of structure is not yet clear, and the pore size and porosity of bladder acellular matrix cannot be artificially controlled