Biological material for repairing bladder tissue and preparation method thereof

A biomaterial and tissue repair technology, which is applied in the field of composite biomaterials for bladder tissue repair and its preparation, can solve problems such as the influence of biological active factors, disordered and irregular arrangement, sparse smooth muscle cells, etc., and achieves good biocompatibility and mechanical properties. The effect of excellent performance and significant application value
CN113304319AInactive Publication Date: 2021-08-27NANJING FIRST HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING FIRST HOSPITAL
Publication Date
2021-08-27
Estimated Expiration
Not applicable · inactive patent
Patent Text Reader

Abstract

The invention provides a preparation method of a biological material for repairing bladder tissue, which comprises the following steps: selecting gelatin, and carrying out modification treatment to obtain methacrylic acid acylated hydrogel; selecting hyaluronic acid, and carrying out modification treatment to obtain methacrylic acid acylated hyaluronic acid; selecting and preparing a bladder acellular matrix, and performing modification treatment to obtain a methacrylic acid acylated bladder acellular matrix; and mixing the three materials in proportion and promoting in-situ cross-linking reaction to obtain the biological material. According to the biological material obtained by the invention, most of the components are derived from the bladder, biological active factors of the bladder are reserved, and the biological material has relatively high biological activity and good biocompatibility and can promote regeneration of bladder urinary tract epithelial cells, bladder smooth muscle cells, Cajal interstitial cells and human umbilical vein endothelial cells; and compared with the traditional biological material, the mechanical property is more excellent.
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Description

technical field

[0001] The invention relates to the technical fields of preparation of biomedical materials and tissue engineering, in particular to a composite biomaterial for bladder tissue repair and a preparation method thereof. Background technique

[0002] The basement membrane is an important extracellular matrix present in all epithelial and endothelial tissues that maintains tissue integrity and acts as a barrier between different cells and molecules, separating different tissue types, and has mechanical Signal transmission and other functions. The main components of the basement membrane are laminin, type IV collagen, heparan sulfate proteoglycan, and various growth factors, cytokines, chemokines, and proteases. BME / matrigel, which is widely used at present, is a basement membrane extract isolated from epithelial tumors, and its composition is similar to that of the real basement membrane. However, BME / matrigel has certain disadvantages: 1) Since BME / matrigel is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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