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Application of plantain polysaccharide in preparation of anti-porcine pseudorabies virus infection preparation

A porcine pseudorabies and virus infection technology, applied in the field of biomedicine, to achieve the effect of reducing cell lesions, significant effect, and rich drug sources

Active Publication Date: 2022-03-29
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have isolated and purified the macromolecular component from plantain—plantago asiatica polysaccharide (PLP). It is reported that plantain polysaccharide has antioxidant, bactericidal, anti-inflammatory, anti-tumor effects, but for plantain polysaccharide Antiviral activity has not been reported

Method used

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  • Application of plantain polysaccharide in preparation of anti-porcine pseudorabies virus infection preparation
  • Application of plantain polysaccharide in preparation of anti-porcine pseudorabies virus infection preparation
  • Application of plantain polysaccharide in preparation of anti-porcine pseudorabies virus infection preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation against porcine pseudorabies virus infection is obtained by dissolving plantain polysaccharide in 0.01mol / L PBS buffer solution, and the concentration of plantain polysaccharide is 50mg / mL.

Embodiment 2

[0030] Effects of plantain polysaccharides on cytopathic effects caused by porcine pseudorabies virus

[0031] PK-15 cells were digested with 0.25% trypsin, diluted with DMEM nutrient solution containing 5% fetal bovine serum and counted, spread into six-well plates at 5×10 5 The concentration of cells per well is appropriate, placed at 37°C, 5% CO 2 After culturing in an incubator until the cells grew to a density of 70-80% (about 17-18h), the original culture medium was discarded, and the cells were washed three times with PBS solution. / mL, 200μg / mL, 400μg / mL, 600μg / mL) after pretreatment for 1h, add PRV XJ5 strain (0.1MOI) to infect the cells, set at 37°C, 5% CO 2 One hour after infection in the incubator, wash three times with PBS solution, replace each well with DMEM nutrient solution containing 2% fetal bovine serum, infect for 24 hours and the drug has been present, observe the morphological changes and pathological changes of the cells under a microscope. according ...

Embodiment 3

[0033] The activity of plantain polysaccharides inhibiting porcine pseudorabies virus infection on PK-15 cells (Western blot, IFA and TCID 50 experiment)

[0034] (1) Western blot determination of the activity of plantain polysaccharides in inhibiting porcine pseudorabies virus infection on PK-15 cells

[0035] PK-15 cells were digested with 0.25% trypsin, diluted with DMEM nutrient solution containing 5% fetal bovine serum, and added dropwise to a 6-well plate at 5×10 5 / well concentration is appropriate, placed at 37 °C, 5% CO 2Cultivate in the incubator until the cells adhere to the wall and form a monolayer (about 17-18h), discard the original culture medium, wash the cells three times with PBS solution, suck up the residual liquid, add 1mL serum-free DMEM to each well and corresponding concentrations of plantain polysaccharides (100 μg / mL, 200 μg / mL, 400 μg / mL, 600 μg / mL), and PK-15 cells at 37 ° C, 5% CO 2 After incubating in the incubator for 1 h, infect the cells wi...

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Abstract

The present invention provides the application of a plantain polysaccharide in the preparation of an anti-porcine pseudorabies virus infection preparation, in particular, relates to the use of a plantain polysaccharide in the preparation of a preparation for preventing and / or treating porcine pseudorabies virus infection The application belongs to the antivirus application field. In vitro cell experiments show that the plantain polysaccharide can effectively inhibit porcine pseudorabies virus infection, and can significantly reduce the cytopathy caused by porcine pseudorabies virus. By adding plantain polysaccharides at different stages of virus infection, it was found that plantain polysaccharides mainly inhibited the adsorption and entry of viruses to cells. The plantain polysaccharide of the present invention has the advantage that it belongs to the extract of traditional Chinese medicine, has little toxic and side effects, and has high safety.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of a plantain polysaccharide in the preparation of an anti-porcine pseudorabies virus infection preparation. Background technique [0002] Pseudorabies (PR), also known as AujeszKy's disease, is a variety of diseases caused by porcine herpesvirus 1 (SuHV-1) or pseudorabies virus (PRV). An acute viral infectious disease characterized by fever, severe itching (except in pigs), respiratory and nervous system disease, and encephalomyelitis. Transmission route is very extensive, and its main mode of transmission is direct contact transmission, also can spread this disease by the air droplet that contains virus. [0003] Porcine pseudorabies virus belongs to the family Herpesviridae, subfamily Alphaherpesvirus, and porcine herpesvirus type I of the genus Vesivirus. It is mainly composed of capsule, capsid and core. The shape is oval or round, and its particle diame...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K36/68A61P31/22A23K20/163A23K50/30
CPCA61K31/715A61K36/68A61P31/22A23K20/163A23K50/30
Inventor 郇长超徐瑶于亚玲高崧
Owner YANGZHOU UNIV
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