Biomarkers and their applications for nmosd prediction or recurrence monitoring

A biomarker and protein technology, applied in the field of biomarkers, can solve problems such as inability to meet clinical needs, and achieve the effect of improving clinical services

Active Publication Date: 2021-11-30
BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no recognized biomarker with high predictive value for the onset and disease progression of NMOSD so far, which cannot meet the clinical needs

Method used

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  • Biomarkers and their applications for nmosd prediction or recurrence monitoring
  • Biomarkers and their applications for nmosd prediction or recurrence monitoring
  • Biomarkers and their applications for nmosd prediction or recurrence monitoring

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: The clinical data collection of NMOSD patient and healthy control group

[0056] This study included inpatients in Beijing Tiantan Hospital Affiliated to Capital Medical University from October 2018 to November 2019. Inclusion criteria for NMOSD patients: (1) The age of onset is between 18 and 80; (2) NMOSD patients are clearly diagnosed according to the 2015 Wingerchuk NMOSD diagnostic criteria; (3) Blood AQP4 antibody positive; (4) This is an acute attack : (a) Neurological symptoms such as optic neuritis, transverse myelitis, or acute brain injury that appear newly or are significantly worse than before (i.e. patients must seek medical attention within 7 days after the onset of symptoms). (b) New-onset symptoms must persist for at least 48 hours and not be attributable to other clinical factors (eg, fever, infection, injury, adverse reaction to concomitant medication). (c) New onset symptoms must be consistent with objective clinical signs of sensory, ...

Embodiment 2

[0057] Example 2: Sample collection and storage of NMOSD patients and healthy controls

[0058] 1. Collection of plasma: Collect peripheral blood in purple tubes containing anticoagulant EDTA or heparin, and centrifuge within 30 minutes after sample collection, at 3000 rpm for 10 minutes, at 2-8°C. The upper plasma layer was collected and stored in batches at -80°C. Avoid repeated freezing and thawing of samples. Note: Samples should be centrifuged sufficiently to avoid hemolysis or the presence of particles.

[0059] 2. Extraction of exosomes in peripheral plasma

[0060] Astrocyte-derived exosomes in plasma were extracted by immunosorbent assay.

[0061] 2.1 Magnetic beads (Beads) coated antibody

[0062] 1) Add 100ug of astrocyte surface glutamate transporter antibody (EAAT2-IgG) into the ultrafiltration tube and wash repeatedly;

[0063] 2) Add 8-10ul of biotin (Biotin) with a final concentration of 10mM to the IgG, and let it stand at room temperature for 1-2 hours; ...

Embodiment 3

[0084] Example 3: Proteomics technology analysis, screening out differentially expressed proteins, and obtaining a detection combination of prognostic biomarkers

[0085] 1. The present invention uses the next-generation non-labeled quantitative proteomics technology to complete the analysis. In the data independent acquisition (DIA) mode, it can provide unparalleled proteome coverage and realize the detection of a large number of proteins in each sample at the same time. Precise, highly reproducible quantification. The DIA process provides an ideal platform for qualitative analysis of differentially expressed proteomes or quantitative proteomes of massive samples. The DIA process is based on three necessary steps:

[0086] 1) Construct a spectral library: The spectral library collects all detectable non-redundant high-quality peptide information (MS / MS spectra) of the sample, and serves as a peptide identification template for subsequent data analysis. It contains fragment ...

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Abstract

The invention discloses biomarkers for NMOSD prediction or recurrence monitoring and applications thereof. By preparing reagents or kits capable of detecting and analyzing the expression level of the biomarkers, and detecting the corresponding biomarkers in the peripheral blood or plasma exosomes of the subjects, the prediction and treatment of neuromyelitis optica spectrum diseases can be realized. Monitoring can not only predict the risk of the subject suffering from neuromyelitis optica spectrum disease, but also monitor the recurrence of neuromyelitis optica spectrum disease in patients or subjects. The present invention provides biomarkers for NMOSD prediction or recurrence monitoring, which helps to better understand the pathophysiology of NMOSD, thereby improving clinical services for NMOSD patients.

Description

technical field [0001] The present invention relates to biomarker technology, in particular to biomarkers that can be used for NMOSD analysis. Background technique [0002] Neuromyelitis optica spectrum disease (NMOSD) is an immune demyelinating disease of the central nervous system, mainly acute or subacute demyelinating lesions involving simultaneous or sequential involvement of the optic nerve and spinal cord. For a long time, there has been controversy over whether NMOSD is an independent disease or a variant of multiple sclerosis (MS). Since Lennon et al. (2004) discovered aquaporin-4 (AQP4) antibodies in the serum of neuromyelitis optica (NMO) patients, NMOSD, as an independent disease different from MS, is a humoral immunity-based disease. The main autoimmune diseases of the central nervous system have been generally recognized. The mechanism is that the specific antibody produced by sensitized B lymphocytes binds to complement, deposits and destroys AQP4 on the sur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/285
Inventor 施福东金薇娜李昕頔田德财魏常娟
Owner BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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