Biomarker for ICH prognosis evaluation and application thereof

An exosome and plasma technology, applied in the field of medical biology, can solve the problems of inability to meet clinical needs and the high value of ICH prognosis prediction, and achieve the effect of improving clinical services.

Active Publication Date: 2022-01-25
BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no recognized biomarker with high predictive value for the prognosis of ICH so far, which cannot meet the clinical needs

Method used

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  • Biomarker for ICH prognosis evaluation and application thereof
  • Biomarker for ICH prognosis evaluation and application thereof
  • Biomarker for ICH prognosis evaluation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Clinical data collection of ICH patients and health control group

[0066] This study was included in the patients who were hospitalized in Beijing Tiantan Hospital affiliated to Capital Medical University from October 2018 to the Patients of Patients, Beijing Tiantan Hospital, November 2019. ICH patient inclusion standard: (1) The age of onset is between 18-80; (2) According to the patient's clinical and imaging data, it is clearly diagnosed with acute cerebral hemorrhage; (3) This disease is due to hypertensiveness; ) The bleeding position is around the foundation, and the bleeding is not broken into the brain, the bleeding volume is less than 50 ml. The clinical information includes: patients generally basic information and clinical characteristics, high risk of martial arterygene, high blood pressure, diabetes, coronary heart disease, high blood fat, etc.), acute period treatment plan. Follow-up information includes: March, June 1, NIHSS and MRS scores, recu...

Embodiment 2

[0067] Example 2: Sample Collection and Storage of ICH Patients and Health Control Group

[0068] 2.1 Plasma Collection - X Peripheral Blood is collected in a purple tube containing anti-condensing agent EDTA or heparin, centrifuged in 30 min after sample collection, 3000 rpm 10min, 2-8 ° C. The upper plasma was collected, stored in batches to -80 ° C. Sample avoids repeated freeze-thaw. Note: The sample should be sufficiently centrifuged to avoid hemolysis or particle presence.

[0069] 2.2 Extraction of Sino-Foreout in Peripheral Plasma

[0070] We use serum and plasma EXOQUICKOBOFO Separation kits (item numbers: Equltra-20A-1), an innovative kit with expertise in exosomes.

[0071] 2.2.1 extraction

[0072] 1) Remove the sample, 3000g, and 15 min from centrifugation.

[0073] 2) If there is still a cell debris residue, the supernatant is once again at a position 12,000g, 10 min, and the supernatant is transferred to a new centrifuge tube.

[0074] 3) After obtaining 250 μL sam...

Embodiment 3

[0100] Example 3: Gene, proteolytic technology analysis, screening of differentially expressing proteins, resulting in recurrence, prognosis Detection combination of material tag

[0101] 3.1 This project uses the next generation of non-marked quantitative proteomics techniques to complete analysis, in Data Independent Acquisition, DIA mode, it provides unparalleled protein group coverage while achieving exact amount of protein per sample , Highly repeatable quantification. The DIA process provides an ideal differentially expressing protein group qualitative analysis or a protein set quantification platform of massive samples. The DIA process is based on three necessary steps:

[0102] 1) Construction Spectral Stock: Spectral Library Collect all detectable non-redundant high quality peptide information (MS / MS spectrum), as a peptide identification template for subsequent data analysis. It includes a fragment ion intensity and retention time describing the peptide peak characte...

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Abstract

The invention provides a biomarker for ICH prognosis evaluation and application thereof. Specifically, the invention provides application of a reagent for detecting at least one peripheral plasma or exosome protein or at least one exosome micro RNA in preparation of a reagent or a kit for predicting, diagnosing or monitoring transient ischemic attack. According to the technical scheme provided by the invention, a new biomarker group is formed, so that the pathophysiology of ICH can be better known, a new opportunity is provided for diagnosis and prognosis, and the clinical service of ICH patients is improved.

Description

Technical field [0001] The present disclosure relates to the field of medical biology. Specifically, the present disclosure relates to biomarkers, and they are used in diagnosing brain injury or brain-related injuries, especially in the application of cerebral hemorrhage, ICH, and involving biomarkers in individuals Methods of detecting, predicting or monitoring cerebral hemorrhage. Background technique [0002] InTracerebral Hemorrhage, ICH, refers to bleeding caused by vascular rupture in non-foreign brain, accounting for 20% to 30% of all stroke, and acute periodical mortality is 30% ~ 40%. The cause of the occurrence is related to the ventricular vessel lesions, that is, closely related to high blood lipids, diabetes, high blood pressure, blood vessels, smoking, etc. Patients with cerebral hemorrhage often due to emotional excitement, sudden pathogenesis, the early mortality rate is high, and most of the survivors have different degrees of exercise disorders, cognitive disord...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68C12Q1/6883
CPCG01N33/6893C12Q1/6883C12Q2600/118C12Q2600/158C12Q2600/178G01N2800/2871G01N2800/52G01N2800/60
Inventor 施福东王拥军金薇娜
Owner BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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