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Biomarker for prognosis or recurrence early warning evaluation of acute ischemic stroke and application of biomarker

A technology of ischemic stroke and biomarkers, applied in the field of biomarkers, can solve the problems of inability to meet clinical needs, high prognosis prediction value of AIS, and achieve the effect of improving clinical services

Active Publication Date: 2022-02-08
BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no recognized biomarker with high predictive value for the prognosis of AIS so far, which cannot meet the clinical needs

Method used

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  • Biomarker for prognosis or recurrence early warning evaluation of acute ischemic stroke and application of biomarker
  • Biomarker for prognosis or recurrence early warning evaluation of acute ischemic stroke and application of biomarker
  • Biomarker for prognosis or recurrence early warning evaluation of acute ischemic stroke and application of biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1: Collection of clinical data of acute ischemic stroke patients and healthy control groups

[0076] This study included inpatients in Beijing Tiantan Hospital Affiliated to Capital Medical University from October 2018 to November 2019 and patients in the multicenter China National Stroke Registry Study-Ⅲ (CNSR-3). Inclusion criteria for patients with acute ischemic stroke: (1) The age of onset is between 18 and 80; (2) Patients with acute cerebral infarction are clearly diagnosed according to the patient's clinical and imaging data; (3) The cause of the onset is aortic Atherosclerotic; (4) There is symptomatic intracranial or extracranial arterial stenosis (decrease in arterial lumen diameter ≥ 50%). The included clinical information includes: general basic information and clinical characteristics of patients, high risk factors for aortic atherosclerosis (hypertension, diabetes, coronary heart disease, hyperlipidemia, etc.), and acute phase treatment plan. Fol...

Embodiment 2

[0077] Example 2: Collection and storage of samples from patients with acute ischemic stroke and healthy controls

[0078] 1. Collection of plasma

[0079] Collect peripheral blood in purple tubes containing anticoagulant EDTA or heparin, and centrifuge within 30 minutes after sample collection, at 3000 rpm for 10 minutes, at 2-8°C. The upper plasma layer was collected and stored in batches at -80°C. Avoid repeated freezing and thawing of samples. Note: Samples should be centrifuged sufficiently to avoid hemolysis or the presence of particles.

[0080] 2. Extraction of exosomes in peripheral plasma

[0081] ExoQuick Extracellular Vesicle Isolation Kit (Catalog No.: EQULTRA-20A-1) for serum and plasma was used for the detection.

[0082] 2.1 Extraction

[0083] 1) Take out the sample, centrifuge at 3000g for 15min to get the supernatant.

[0084] 2) If there are still cell debris remaining, centrifuge the supernatant again at 12,000g for 10min, and transfer the supernatan...

Embodiment 3

[0111] Example 3: Analysis of genes and proteomics, screening out differentially expressed proteins, and obtaining the detection combination of biomarkers for recurrence and prognosis assessment

[0112] 1. This project uses the next generation of non-labeled quantitative proteomics technology to complete the analysis. In the data-independent acquisition (Data independent acquisition, DIA) mode, it can provide unparalleled proteome coverage, while achieving accurate, Highly reproducible quantification. The DIA process provides an ideal platform for qualitative analysis of differentially expressed proteomes or quantitative proteomes of massive samples. The DIA process is based on three necessary steps:

[0113] 1) Construction of a spectral library: The spectral library collects all detectable non-redundant high-quality peptide information (MS / MS spectra) of the sample, which is used as a peptide identification template for subsequent data analysis. It includes fragment ion i...

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PUM

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Abstract

The invention discloses a biomarker for prognosis or recurrence early warning evaluation of acute ischemic stroke and application of the biomarker. According to the biomarker provided by the invention, an ELISA reagent and an RT-qPCR reagent for detecting the biomarker are prepared, and the expression quantity of the corresponding biomarker in plasma protein or plasma exosome protein of a subject is detected by using the reagents, so that the acute ischemic stroke is analyzed or detected; and the risk of a subject suffering from acute ischemic cerebral apoplexy can be predicted, and occurrence of acute ischemic cerebral apoplexy of a patient or the subject can be monitored. The invention provides the biomarker for prognosis or recurrence early-warning evaluation of acute ischemic cerebral apoplexy, and the biomarker is beneficial to better understanding of pathophysiological mechanisms of acute ischemic cerebral apoplexy.

Description

technical field [0001] The present invention relates to the technical field of biomarkers, in particular to biomarkers used for the prognosis or early warning evaluation of acute ischemic stroke. Background technique [0002] Cerebrovascular disease (Cerebralvascular disease, CVD) includes transient ischemic attack (Transientischemic attack, TIA) and stroke (Stroke). AIS, also known as acute cerebral infarction, is a group of clinical syndromes in which brain tissue blood supply disorder caused by various reasons leads to ischemia-hypoxic necrosis and neurological dysfunction. AIS is the most common type of stroke, accounting for about 60%-80% of all strokes. However, the treatment of AIS is very limited. The currently clinically proven effective acute treatment options are intravenous alteplase (tPA) thrombolysis or endovascular thrombectomy to achieve vascular recanalization in the ischemic region. However, due to the narrow treatment time window and the increased risk o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68
CPCG01N33/6893G01N2800/2871G01N2800/60G01N2800/54C12Q1/6883C12Q2600/118C12Q2600/158C12Q2600/178G01N2800/52
Inventor 王拥军施福东金薇娜孟霞袁梦张程祎魏常娟
Owner BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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