Preparation method of cell culture medium and automatic equipment

Abstract

The invention provides a preparation method of a cell culture medium and automatic equipment. The preparation method comprises the following steps: grouping of culture medium components, high-temperature grouping sterilization, automatic mixing and automatic packaging, wherein at least the rear three steps, i.e., high-temperature grouping sterilization, automatic mixing and automatic packaging are automatically completed in the same independent closed system. The invention further provides the automatic equipment for the cell culture medium. According to the preparation method of the cell culture medium and the automatic equipment, main adverse reactions of high-temperature sterilization are eliminated, the automation degree is high, and the consumption of scientific research manpower is saved.

Description

Technical field [0001] The present invention belongs to the technical field of cell culture medium, in particular a method for preparing a cell culture medium and automation equipment. Background technique [0002] Cell culture media to simulate natural nutrition environment is the basis of cultured cell experiments, widely used in the field of medical and health research, life sciences, chemistry and pharmacy; sterilizers and other equipment preparation facilities media, is also relevant laboratory or industrial production processes the standard. Currently, the agar medium is the most widely used media types. [0003] The medium components can be divided into serum-free medium and serum-free medium comprising essential components: serum, salts, buffer systems, carbohydrates, amino acids, vitamins, proteins, peptides, fats, trace elements, trace elements, special antibiotic. Wherein, serum albumin, a complex mixture of growth factors and growth inhibitors, cell culture medium is ...

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Problems solved by technology

[0006] 2. Since at least 2014, studies in several mainstream academic journals have pointed out that exposure of culture medium to temperatures exceeding 121°C without proper gelation (referring to agar) may lead to significantly poor growth of cells

[0007] 3. No matter what kind of sterilization technology is used, for most laboratories performing cell culture, medium configuration is very time-consuming; and, in order to eliminate the above-mentioned defects, improve component group sterilization or combine multiple sterilization methods. Bacteria technology also greatly increases the operation steps, which ultimately increases the risk of contamination

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