Method for treating high-concentration cadmium pollution through calcium-enhanced microorganism mineralization and combined phytoremediation

A technology of phytoremediation and calcium enhancement, which is applied in the field of calcium-enhanced microbial mineralization to control high-concentration cadmium pollution and combined phytoremediation. It can solve the problems of long pollution process, pollution, and failure to achieve the repair effect, and achieve simple operation and low cost. The effect of low cost and broad application prospects

Pending Publication Date: 2021-09-21
JIANGSU UNIV
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, phytoremediation of cadmium pollution has been widely studied, but the process of phytoremediation of cadmium pollution is long, and in the process of remediation, heavy metals will still migrate with rainfall or runoff, thus failing to achieve the expected remediation effect
[0004] The Chinese invention patent (CN 107537854 A) discloses a carbonate mineralizing bacterium. It is found through experiments that the bacterium can effectively reduce the content of available cadmium in the soil within a week, but the mineralized precipitate generated by biomineralization has a certain Durability and mineralization bacteria have a certain tolerance, the cadmium carbonate crystals produced by t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for treating high-concentration cadmium pollution through calcium-enhanced microorganism mineralization and combined phytoremediation
  • Method for treating high-concentration cadmium pollution through calcium-enhanced microorganism mineralization and combined phytoremediation
  • Method for treating high-concentration cadmium pollution through calcium-enhanced microorganism mineralization and combined phytoremediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Calcium-intensified conditions to remove high-concentration cadmium-polluted wastewater

[0026] Inoculate the sterilized culture medium with the mother liquor of Bacillus pasteurianum, and shake and cultivate it at 28°C for two days. After the cultivation is completed, use a spectrophotometer to measure the absorbance of the bacterial solution, and adjust the concentration of the bacterial solution to OD by centrifugal concentration and saline 600 =12 for spare;

[0027] Use pure water and cadmium chloride to prepare high-concentration cadmium-containing wastewater with a cadmium concentration of 50 mM, and set aside.

[0028] Experimental group 1: After mixing high-concentration cadmium-containing wastewater, anhydrous calcium chloride, bacterial liquid and urea, add water to configure a 100mL reaction system; in the 100mL reaction system, bacterial liquid, calcium chloride, cadmium ions (Cd 2+ ), the final concentration of urea were OD 600 =1.2, 5mM, 5mM...

Embodiment 2

[0034] Example 2 Microbial mineralization improves soil wheat growth

[0035] Inoculate the sterilized culture medium with Bacillus pasteurianum, and culture it with shaking at 28°C for two days. After the cultivation is completed, use a spectrophotometer to measure the absorbance of the bacterial solution, and adjust the concentration of the bacterial solution to OD by centrifugal concentration and saline 600 =12, spare.

[0036] Preparation of cadmium-contaminated soil samples: 10 mg / L CdCl 2 The solution was mixed well with the soil to ensure 10 µg Cd per gram of soil 2+ . The total volume of prepared contaminated soil was 1.2m 3(depth × width × length = 0.3 m × 2 m × 2 m), the area of ​​each soil sample is 1m 2 , the depth is 30cm. CdCl 2 The solution (336 L×10 mg / L) may pollute the soil.

[0037] Transplant wheat seedlings (stem length less than 10cm) into the soil. Four samples are set in this embodiment:

[0038] T1 is an untreated control group;

[0039] In g...

Embodiment 3

[0044] Example 3 Microbial mineralization remediation of cadmium-contaminated soil

[0045] Inoculate the sterilized culture medium with Bacillus pasteurianum, and culture it with shaking at 28°C for two days. After the cultivation is completed, use a spectrophotometer to measure the absorbance of the bacterial solution, and adjust the concentration of the bacterial solution to OD by centrifugal concentration and saline 600 =12 for backup.

[0046] Take 50 mM high-concentration cadmium-containing wastewater that has been prepared in the laboratory, add fresh soil and stir thoroughly to prepare heavy metal cadmium-contaminated soil, and the concentration of cadmium ions in the soil is 5 mg / kg.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for treating high-concentration cadmium pollution through calcium-enhanced microorganism mineralization and combined phytoremediation. The method is used for passivating high-concentration cadmium (larger than or equal to 5 mM) pollution in soil. The method comprises the following steps: firstly, pasteurella, calcium strengthening liquid and urea are mixed into to-be-remediated soil to immediately passivate high-concentration effective-state cadmium in the soil to form cadmium carbonate precipitate; and then plants are planted. The durability problem of passivation and re-dissolution of cadmium is solved through phytoremediation, passivated heavy metal can be slowly dissolved out along with migration of time and is absorbed by the plants, and compared with a traditional chemical method, passivation is achieved. The method is easy to operate and low in cost, the defects of biomineralization and phytoremediation are mutually compensated, a complete system for stably removing the heavy metal from the soil is formed, and the method has wide application prospects in the aspect of soil heavy metal pollution remediation.

Description

technical field [0001] The application relates to the field of heavy metal polluted water and soil remediation, and specifically relates to a calcium-enhanced microbial mineralization method for treating high-concentration cadmium pollution and combined phytoremediation. Background technique [0002] Cadmium (Cd) is a highly toxic heavy metal mainly used in the production of galvanizing and electroplating, batteries, electrical conductors, alloys, pigments, plastics, and stabilization of phosphate fertilizers (Byrne, C., Divekar, S.D., Storchan , G.B., Parodi, D.A. and Martin, M.B. (2009) Cadmium--a metallohormone Toxicol ApplPharmacol 238(3), 266-271) and other industries. In nature, cadmium exists in the form of Cd(II), which has high solubility and toxicity, and is easy to migrate. In addition, cadmium (II) is stable and difficult to eliminate, and often enters the human body through the food chain, causing damage to the human brain, kidney, bone, lung, liver and reprodu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): B09C1/08B09C1/10
CPCB09C1/08B09C1/105B09C2101/00
Inventor 成亮方龙洋牛其建刘美丽俞洋洋
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap