Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of a lncRNA combined with carcinoembryonic antigen in the diagnosis of lung cancer and the preparation of a lung cancer diagnostic kit

A carcinoembryonic antigen, lung cancer diagnosis technology, applied in the field of disease diagnosis, can solve the problem of low accuracy of lung cancer diagnosis, and achieve the effect of improving the accuracy of diagnosis

Active Publication Date: 2022-05-31
深圳核子华曦医学检验实验室
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] CEA has an important reference value for the diagnosis of lung cancer, but its accuracy in the diagnosis of lung cancer alone is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of a lncRNA combined with carcinoembryonic antigen in the diagnosis of lung cancer and the preparation of a lung cancer diagnostic kit
  • Application of a lncRNA combined with carcinoembryonic antigen in the diagnosis of lung cancer and the preparation of a lung cancer diagnostic kit
  • Application of a lncRNA combined with carcinoembryonic antigen in the diagnosis of lung cancer and the preparation of a lung cancer diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The total RNA extraction kit was purchased from the Invitrogen company in the United States.

[0018] The reverse transcription kit was purchased from ThermoFisher Scientific.

[0019] RT-PCR measuring instrument was purchased from Germany Qiagen company.

[0020] lncRNALINC01108 and internal reference GAPDH primers were designed and synthesized by Beijing Boaosen Company.

[0023] Lung cancer samples: selected from the First Affiliated Hospital of Nanjing Medical University from January 2018 to June 2020, pathologically diagnosed as lung cancer

[0024] The age distribution of the three groups of samples is highly similar, P>0.05, there is no statistical significance.

[0027] Training set: randomly select 32, 35, 35 from lung cancer samples, benign lung lesions samples and healthy population samples.

[0029]

[0036] lncRNALINC01108 gene downstream primer sequence (SequenceNO.2):

[0039] The GAPDH gene downstream primer sequence (Sequence NO.4): 5'-GGCTGTTGTCATACTTCTCATG...

Embodiment 2

[0056] A lung cancer diagnostic kit, comprising serum CEA assay reagent and serum lncRNALINC01108 assay reagent.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of lncRNA combined with carcinoembryonic antigen in the diagnosis of lung cancer and the preparation of a lung cancer diagnostic kit. Those skilled in the art know that AUC≤0.5 means no diagnostic value, 0.5<AUC≤0.7 means low diagnostic accuracy, 0.7<AUC≤0.9 means better diagnostic accuracy, and AUC≥0.9 means highest diagnostic accuracy. In the training set of the present invention, the diagnostic accuracy of CEA alone for distinguishing lung cancer and non-lung cancer is low (AUC=0.736), and the diagnostic accuracy of CEA and lncRNA LINC01108 for distinguishing lung cancer and non-lung cancer is significantly improved (AUC=0.917); In the verification set, the combination of serum lncRNA LINC01108 and serum CEA significantly improved the diagnostic accuracy of lung cancer, and the accuracy of the combined diagnosis of lung cancer was over 90%.

Description

A lncRNA combined with carcinoembryonic antigen in the diagnosis of lung cancer and preparation of a diagnostic kit for lung cancer applications technical field The invention belongs to disease diagnosis field, relate to lung cancer diagnosis, be specifically related to a kind of lncRNA in conjunction with carcinoembryonic antigen in Application in lung cancer diagnosis and preparation of lung cancer diagnostic kits. Background technique Lung cancer is one of the common malignant tumors in my country, and its morbidity and fatality rate are the highest among all kinds of malignant tumors. At present, there is no more reliable serum diagnostic marker. [0003] Carcinoembryonic antigen (carcinoembryonicantigen, CEA) was first introduced by Gold and Freedman in 1965. A tumor-associated antigen extracted from intestinal cancer and embryonic tissues, which is an acidic sugar egg with the characteristics of human embryonic antigen White, present on the surface of cancer ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/574
CPCC12Q1/6886G01N33/57423G01N33/57473C12Q2600/158C12Q2600/178
Inventor 朱阳晨其他发明人请求不公开姓名
Owner 深圳核子华曦医学检验实验室
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com