Total cholesterol detection reagent and detection chip

A technology for total cholesterol and detection reagents, which is used in measurement devices, measurement of color/spectral properties, and material analysis by observing the effect on chemical indicators, can solve the problems of high test results and poor test results. To achieve the effect of simple operation and accurate detection results

Pending Publication Date: 2021-10-12
GENRUI BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the technical problem of poor accuracy of enzymatic detection results, the embodiment of the present invention provides a serum total cholesterol detection reagent and a detection chip, which can detect the TC content in the detection sample by using oxidized coenzyme and cholesterol dehydrogenase, and the reaction process It is not easily interfered by its substances, and the test results are highly accurate

Method used

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  • Total cholesterol detection reagent and detection chip
  • Total cholesterol detection reagent and detection chip
  • Total cholesterol detection reagent and detection chip

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preparation example Construction

[0058] The embodiment of the present invention also provides a preparation method of a total cholesterol detection reagent, the method comprising the following steps:

[0059] S11. Acquiring the first reagent and the second reagent;

[0060] In the embodiment of the present invention, the configuration process of the first reagent is as follows: add 800 ml of distilled water to a 1-liter beaker, weigh the first buffer solution, adjust the pH to be compatible with the first buffer solution, and wait until the first buffer solution is completely dissolved Add oxidized coenzyme I in sequence, and finally add the first excipient, and set the volume to 1 liter. Optionally, in some embodiments, sodium chloride and / or the first surfactant may also be added while adding the oxidized coenzyme I into the beaker.

[0061] The configuration process of the second reagent is as follows: add 800 ml of distilled water to a 1-liter beaker, weigh the second buffer solution, adjust the pH to be...

Embodiment 1

[0069] The total cholesterol detection chip in this embodiment includes a first lyophilized reagent sphere prepared from a first reagent and a second lyophilized reagent sphere prepared from a second reagent.

[0070] The first reagent includes the following components:

[0071]

[0072] The second reagent includes the following components:

[0073]

Embodiment 2

[0075] The difference between this embodiment and embodiment 1 is as follows:

[0076] The first reagent includes the following components:

[0077]

[0078] The first reagent includes the following components:

[0079]

[0080]The performance of the detection chip obtained in Example 1 of the present invention will be described below in conjunction with the table. It should be noted that, at 37° C., the change value of the absorbance of the measurement chip injected into the serum sample at a wavelength of 340 nm was measured by a vp10 portable biochemical analyzer. Calibrate with the calibrator provided by Randox, UK, and calculate the concentration of total cholesterol in the serum sample.

[0081] (1), precision test: the total cholesterol concentration in the serum sample whose known serum total cholesterol concentration is 4.02mmol / L is tested by the detection chip provided in Example 1 of the present invention 20 times, and the measured concentration value is ca...

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Abstract

The invention relates to the technical field of total cholesterol detection, in particular to a total cholesterol detection reagent and a detection chip. According to the total cholesterol detection reagent and the detection chip provided by the embodiment of the invention, cholesterol ester in a detection sample can be hydrolyzed into free cholesterol under the action of cholesterol esterase, the free cholesterol is reduced into cholane-4-ene-3-ketone by cholesterol dehydrogenase, and meanwhile, oxidized coenzyme I is reduced into reduced coenzyme I. The content of total cholesterol in a detection sample can be determined by detecting the absorbance of the reduced coenzyme I, the operation is simple, and the reaction process is not easily interfered by other substances (such as ascorbic acid, bilirubin, hemoglobin, triglyceride and lactic dehydrogenase). Therefore, the detection result is more accurate.

Description

technical field [0001] The invention relates to the technical field of total cholesterol detection, in particular to a total cholesterol detection reagent and a detection chip. Background technique [0002] Serum total cholesterol (Total Cholesterol, TC) refers to the sum of cholesterol contained in all lipoproteins in the blood, including free cholesterol (FC) and cholesteryl ester (CE); among them, FC accounts for about 70% of TC. Cholesterol is a kind of lipid compound, which is an important component in the synthesis of adrenocortical hormone, sex hormone, bile acid and cell membrane. Normal TC content ranges from 3.0 to 5.20 mmol / L. If the concentration of TC in the blood is too high, it can cause serious damage to the cardiovascular and cerebrovascular. [0003] At present, the methods for the determination of TC mainly include chemical colorimetry and enzymatic method. Most chemical colorimetric methods use organic solvents to extract FC and CE in serum, develop co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/3103
Inventor 周慧欣汪晨宇牟健陈明
Owner GENRUI BIOTECH INC
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