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Human cell staining process monitoring system and method

A dyeing process, human cell technology, applied in the field of cell dyeing, can solve the problems of difficult dyeing accuracy, difficult dyeing effect verification, etc., to achieve high stability, avoid cell damage or dyeing failure effect.

Inactive Publication Date: 2021-10-19
重庆德方信息技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing method of manually staining cells, on the one hand, it is difficult to effectively verify the staining effect, on the other hand, it is difficult to use empirical data to improve the overall staining accuracy

Method used

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  • Human cell staining process monitoring system and method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Such as figure 1 As shown, the human cell dyeing process monitoring system includes a storage unit, an input unit, a processing unit, a dyeing unit, a monitoring unit and a reminder unit.

[0036] The storage unit and input unit are integrated on the industrial PC, and the reminder unit is a buzzer. The dyeing unit includes a dripping part, a capping part, a mixing part and a drying part; the dripping part includes a dyeing solution dripping pipeline, a cleaning solution flushing pipeline and a waste liquid collection pipeline, and these pipelines adopt existing pipelines, namely Yes, the opening and closing and flow of each pipeline can be controlled by a microcontroller, which all belong to the prior art and will not be repeated here. The dripping part is used for dripping staining solution and buffer solution to the smear, and is also used for washing excess dyeing solution after staining. The capping part includes a mechanical arm for capping and fixing the smear;...

Embodiment 2

[0046] Different from Embodiment 1, this embodiment also includes a placement unit, the placement unit includes a base from bottom to top, a tilting mechanism and a placement seat; the base is fixed to the turntable; Connected electric telescopic rod, the electric telescopic rod is vertically fixed on the base, the free end of the electric telescopic rod is fixed to the bottom surface of the placement seat; the fan in the drying section faces the placement seat;

[0047] The supplementary treatment plan includes a mixed strategy; after the processing unit controls the dripping part to add the dyeing solution, it analyzes the position and uniformity of the dyeing solution according to the dyeing image. As a result, a mixing strategy is generated to uniformly mix the dye liquor to the standard position. The mixing strategy includes the expansion and contraction of the electric telescopic rod, the rotation angle of the turntable motor, and the gear position of the fan; after that,...

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Abstract

The invention belongs to the technical field of cell staining, and particularly relates to a human cell staining process monitoring system and method. The system comprises a storage unit, an input unit, a processing unit, a staining unit and a monitoring unit; the input unit is used for inputting a cell type; the processing unit is used for matching a corresponding staining scheme and an ideal observation sample according to the cell type, and is also used for controlling the staining unit to stain the cells according to the staining scheme; the monitoring unit is used for collecting a cell staining image when the staining unit works; the processing unit is further used for judging whether a supplementary processing scheme is generated or not according to the cell staining image, if so, generating the supplementary processing scheme, and controlling the staining unit according to the supplementary processing scheme; the processing unit is further used for receiving cell imaging during smear observation and optimizing the cell staining scheme according to the corresponding ideal observation sample. According to the method, the staining scheme can be optimized by effectively utilizing the staining process data, so that the staining scheme is more accurate.

Description

technical field [0001] The invention belongs to the technical field of cell staining, in particular to a monitoring system and method for human cell staining process. Background technique [0002] Before cell detection, in order to facilitate the observation of cells, it needs to be stained. [0003] In order to ensure the staining effect of cells, the staining process of cells includes the following process: evenly smear the specimen on the glass slide to form a single layer of uniform coating (called smear, such as blood, bone marrow, etc.), and the smear is made of special The coating is fixed and dried, and then the staining solution and buffer solution are sequentially dripped on the smear so that the dyeing solution and buffer solution are in full contact with the coating for staining (to stain the nucleus and cytoplasm in different colors). After staining, wash Rinse the stained smear with liquid to rinse off excess dye, and then dry the rinsed smear. Afterwards, a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G05B19/048G01N1/30G01N1/31
CPCG05B19/048G01N1/30G01N1/31
Inventor 李春林
Owner 重庆德方信息技术有限公司
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