Active polypeptide-PDRN composition and application thereof in preparation of nursing products
A-PDRN, active polypeptide technology, applied in the field of oral care products, can solve the problems of cumbersome extraction of active ingredients, difficult animal cell culture, unclear structure of active ingredients, etc., to enhance cell penetration and prevent recurrent oral cavity Ulcer, the effect of regulating cellular immunity
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Embodiment 1
[0063] The construction of embodiment 1 protamine-PDRN complex
[0064] First, 30 mg / L protamine solution and 30 mg / L PDRN solution were mixed, and stirred at room temperature for 1 h; then the mixture was allowed to stand at room temperature for 1 h; finally, the mixture was freeze-dried to obtain a protamine-PDRN complex.
Embodiment 2
[0065] Example 2 Construction of Percyganan-antimicrobial peptide LL37-protamine-PDRN complex
[0066] First, mix the solution containing 5mg / L Percyganan, 10mg / L antibacterial peptide LL37 and 10mg / L protamine with the 30mg / LPDRN solution, and stir at room temperature for 1h; then let the mixture stand at room temperature for 1h; finally mix the The solution was freeze-dried to obtain the Percyganan-antimicrobial peptide LL37-protamine-PDRN complex.
Embodiment 3
[0067] Example 3 Construction of Percyganan-antimicrobial peptide LL37-defensin HNP1-protamine-PDRN complex
[0068] First, mix the solution containing 5mg / L Percyganan, 5mg / L antimicrobial peptide LL37, 5mg / L defensin HNP1 and 15mg / L protamine with 40mg / L PDRN solution, and stir at room temperature for 1h; Stand at room temperature for 1 hour; finally, freeze-dry the mixture to obtain the Percyganan-antimicrobial peptide LL37-defensin HNP1-protamine-PDRN complex.
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