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Application of long-chain non-coding RNA in preparation of esophageal squamous cell carcinoma precancerous lesion early warning detection reagent

A long-chain non-coding, esophageal squamous cell carcinoma technology, which is used in the early warning detection reagent or kit for esophageal squamous cell carcinoma precancerous lesions and its application field, can solve the problems of a large population base and the inability to perform endoscopic examinations in the age-appropriate population, and reduce the incidence rate. , The effect of easy promotion and high compliance

Pending Publication Date: 2021-12-03
南京鼓楼医院集团宿迁医院有限公司
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Problems solved by technology

At present, the early diagnosis and screening of esophageal cancer mainly relies on digestive endoscopy. However, the population base in my country is huge, and endoscopy cannot be performed on all the population of the right age. Therefore, risk assessment of esophageal squamous cell carcinoma is currently required for all adults. Screening, further endoscopy for high-risk groups
However, the current risk assessment methods do not have molecular biological targets that can warn of tumors in the early stages of esophageal cancer.

Method used

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  • Application of long-chain non-coding RNA in preparation of esophageal squamous cell carcinoma precancerous lesion early warning detection reagent
  • Application of long-chain non-coding RNA in preparation of esophageal squamous cell carcinoma precancerous lesion early warning detection reagent
  • Application of long-chain non-coding RNA in preparation of esophageal squamous cell carcinoma precancerous lesion early warning detection reagent

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[0021] In order to make the technical problems, technical solutions and beneficial effects solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0022] (1). Sample collection

[0023] Endoscopic and surgical resection samples of different lesion stages, as well as surrounding normal tissue and blood samples were collected in Suqian People's Hospital of Nanjing Drum Tower Hospital Group and Nanjing First Hospital Affiliated to Nanjing Medical University to enrich the sample library. Pathological diagnosis was performed on the samples, and case information and follow-up contacts were established.

[0024] (2).Real-Time PCR to expand the sample size to verify the differentially expressed HUESCC-LncRNA2 in samples of various stages of cancer progression

[0025] Total RNA was extracted using Trizol reagent (Invitrogen). After RNA quantification and purity analysis ...

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Abstract

The invention discloses application of long-chain non-coding RNA in preparation of an esophageal squamous cell carcinoma precancerous lesion early warning detection reagent or kit. According to the invention, the expression level of HUESCC-LncRNA2 in an obtained blood sample is detected, screening and diagnosis are carried out in advance, early detection and early treatment are implemented, the early diagnosis rate is improved, and the morbidity of ESCC is reduced. The detection method is easier to accept by a subject, the compliance of a person to be detected is high, and the method is convenient to popularize.

Description

technical field [0001] The invention relates to the field of tumor detection, more specifically, it relates to a long-chain non-coding RNA HUESCC-LncRNA2 used in early warning detection reagents or kits for esophageal squamous cell carcinoma precancerous lesions and its application. Background technique [0002] Esophageal cancer (EC) is a common multiple malignant tumor that seriously threatens human health. Worldwide, the main pathological type of esophageal cancer——esophageal squamous cell carcinoma (ESCC) accounts for more than 90% of all patients with EC in my country. Also dominated by squamous cell carcinoma, and the incidence rate is still on the rise. In recent years, after multidisciplinary treatment, the treatment effect of esophageal cancer has improved, but the long-term survival benefit of patients is still very limited. In 2015, the New England Journal of Medicine reported that the 5-year survival rate of EAC patients abroad is about 17%, while The overall 5-y...

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6851
CPCC12Q1/6886C12Q1/6851C12Q2600/158C12Q2600/178C12Q2600/118C12Q2521/107C12Q2531/113
Inventor 刘子豪
Owner 南京鼓楼医院集团宿迁医院有限公司
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