The invention discloses a digestive endoscopy biopsy cap. The digestive endoscopy biopsy cap comprises a rectangular sleeve fixedly arranged at the end part of an endoscope, first through grooves areformed in the two sides, close to the top, of the rectangular sleeve; the inner walls of the first through grooves are connected with sealing plates in a sliding manner; grooves are formed in the upper surfaces, close to the opposite ends, of the two sealing plates; the two grooves can form a complete circular-truncated-cone-shaped groove, circular truncated cone blocks are movably connected to the inner walls of the grooves, through holes are formed in the upper surfaces of the circular truncated cone blocks, lifting pipes are fixedly connected to the upper surfaces of the circular truncatedcone blocks through the through holes, and biopsy needles are connected to the inner walls of the lifting pipes in a sliding mode. According to the invention, the above structures are matched for use;the digestive endoscopy biopsy cap can solve the problems that in the actual use process, due to the fact that during biopsy operation, instruments such as a biopsy needle intervene through the biopsy cap, the sealing performance of a traditional biopsy cap is prone to being reduced, substances such as body fluid in the internal environment of a patient are sprayed out, the operation environmentand the instruments are polluted, and inconvenience is brought to operation of doctors.