Novel oligopeptide, and pharmaceutical composition for preventing or treating cancer, comprising same as active ingredient

A composition and oligopeptide technology, which is applied in the direction of medical preparations containing active ingredients, drug combinations, peptide/protein components, etc., can solve the problems of antibody immune response concerns and low efficiency, and achieve small molecular weight and induce cell apoptosis , the effect of less worries

Pending Publication Date: 2021-12-10
L BASE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Meanwhile, although antibodies targeting specific tumor antigens of tumor cells have been developed, antibodies still suffer from concerns about immune response and inefficient penetration into tissues

Method used

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  • Novel oligopeptide, and pharmaceutical composition for preventing or treating cancer, comprising same as active ingredient
  • Novel oligopeptide, and pharmaceutical composition for preventing or treating cancer, comprising same as active ingredient
  • Novel oligopeptide, and pharmaceutical composition for preventing or treating cancer, comprising same as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Embodiment 1: the preparation of oligopeptide

[0107] 1.1. General responses

[0108] All reactions were performed using commercially available materials and reagents without additional reactions unless otherwise stated. All oligopeptide products were specified using liquid chromatography tandem mass spectrometry (LC-MS / MS), high performance liquid chromatography (HPLC), and / or sequencing.

[0109] Specifically, LC-MS / MS and de novo sequencing were performed using an LTQ XL MS / MSn spectrometer (Thermo Fisher Scientific, USA) and Discoverer 2.3 (Thermo Fisher Scientific). HPLC uses Agilent1100HPLC system (detector, VWD detector, 220nm; Column, Waters C18, 4.6mm i.d.X250mm, 5μm; A, 0.1% TFA in water; B, 0.1% TFA in ACN, v / v; flow rate, 1mL / min; gradient, B: 5%-35%, 5 Within minutes, B: 35%-65%, within 15 minutes). All analyzes were commissioned by SEWON BIOTECH.

[0110] 1.2. Synthesis and confirmation of 6 oligopeptides

[0111] Oligopeptides having amino acid ...

Embodiment 2

[0122] Example 2: Confirmation of oligopeptide anticancer effect

[0123] 2.1. The effect of inhibiting lung cancer cell proliferation and inhibiting colony formation

[0124] The MTT assay and the clonogenic assay described in the experimental methods used the oligopeptides AQTITGKT, AQTLTGKT, AQTATGKT, AQTMTGKT, AQTPTGKT or AQTVTGKT according to the present invention to treat non-small cell lung cancer cell lines A549, H1650 and H1975 at a concentration of 100 μM or 500 μM, respectively. After treatment, the degree of colony formation of cancer cells is measured or cytotoxicity is compared.

[0125] results, such as Figures 1 to 3 As shown, when the cell lines were treated with AQTATGKT, AQTMTGKT, AQTPTGKT or AQTVTGKT, statistically significant cytotoxicity and colony formation inhibitory effects were observed compared to the control. This result shows that the oligopeptide according to the present invention has anti-cancer ability capable of inhibiting the growth and p...

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Abstract

The present invention relates to a novel oligopeptide, and a pharmaceutical composition for preventing or treating cancer, comprising same as an active ingredient. The oligopeptide of the present invention has an advantage in that the molecular weight thereof is less than that of an antibody, and thus the risk of an immune response is low, and it is easy for same to penetrate into tissue. The oligopeptide can exhibit the effect of inhibiting the proliferation of malignant tumors by selectively acting on cancer cells or cancer tissue.

Description

technical field [0001] The present invention relates to a novel oligopeptide, a pharmaceutical composition for preventing or treating cancer containing the oligopeptide as an active ingredient and a preparation method thereof. [0002] This application claims priority to U.S. Provisional Application No. 62 / 842,155, filed May 2, 2019, and the entire disclosure in the specification and drawings of that application is incorporated by reference in its entirety. Background technique [0003] Currently, although cancer treatment effects are improving due to the development of early diagnosis methods for cancer and the continuous development of new anti-cancer treatments, cancer remains an important disease that ranks first and second among the causes of death in Korea. Most of the currently used anticancer drugs are based on chemotherapy, and their pharmacological effects vary depending on the type of cancer, and their side effects due to toxicity also vary, which is pointed out t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P35/00A61P35/02
CPCC07K7/06A61P35/00A61P35/02A61K38/00C07K14/4748A61K38/08
Inventor 全道龙禹相赫文昌训郑至恩辛贤熙李慧琳李智英
Owner L BASE CO LTD
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