Method for efficiently inducing human umbilical cord mesenchymal stem cells to be differentiated into hepatocytes and application

A technology of mesenchymal stem cells and human umbilical cord, applied in the field of stem cells and regenerative medicine, can solve the problems of immature liver cell function, low yield of target cells, shorten the induction period, etc., and achieve mature cell function, high induction efficiency, and abundant sources Effect
CN113789296AActive Publication Date: 2021-12-14NO 1 THE PEOPLES HOSPITAL HUAIAN CITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NO 1 THE PEOPLES HOSPITAL HUAIAN CITY
Publication Date
2021-12-14

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Abstract

The invention discloses a method for efficiently inducing differentiation of human umbilical cord mesenchymal stem cells into hepatocytes and an application. The method comprises the following steps: transfecting constructed human umbilical cord mesenchymal stem cells stably expressing long-chain non-coding RNA LINC5657, and a culture medium containing cytokines HGF, bFGF, EG and OSMby lentivirus. The hepatocyte induced differentiation culture medium is replaced once every three days, the hepatocyte-like cells (iHeps) with the hepatocyte morphology and function can be obtained only in about 21 days, the cell function is more mature than that of a traditional method, and a reliable cell source can be provided for clinical hepatocyte transplantation.
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Description

technical field

[0001] The invention belongs to the technical field of stem cells and regenerative medicine, and mainly relates to a method for efficiently differentiating human umbilical cord mesenchymal stem cell lines overexpressing LINC5657 into hepatocytes in a culture medium containing inducers HGF, EGF, bFGF and OSM. Background technique

[0002] Various end-stage liver diseases often lead to liver failure, causing great harm to human health, and are one of the main causes of death worldwide. At present, liver cell transplantation is considered to be one of the important methods for the treatment of acute and chronic liver failure, but the lack of enough donors limits its wide application in clinical practice.

[0003] With the rapid development of stem cell technology in recent years, adult stem cells provide an unlimited source of primary cells for in vitro research. Among adult stem cells, mesenchymal stem cells have the most application prospects, which have the ...

Claims

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