Method for improving germplasm creation efficiency of radish

A germplasm and efficiency technology, applied in the field of plant breeding, to achieve the effect of improving resistance signal transduction and resistance level, improving efficiency and high grafting success rate

Active Publication Date: 2022-01-21
SHANDONG WEIFANG ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] But, there is no report yet about carrying out microspore culture to improve embryo emergence rate after grafting radish with bolting branch of radish

Method used

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  • Method for improving germplasm creation efficiency of radish

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Effect test

Embodiment 1

[0047] Embodiment 1: a kind of method that improves the efficiency of radish germplasm creation, specifically comprises the following steps:

[0048] S1, preparation of rootstock and scion: choose the hybrid variety with strong resistance and easy germination as the rootstock material, select the hybrid variety with beautiful appearance, good quality, crisp green skin color and light spicy taste as the scion material, and mix the rootstock and scion Seeds are sown in 50-hole seedling trays in mid-December and placed in a solar greenhouse; when the temperature is low, a small shed is built in the greenhouse and covered with a second film to control the temperature of the greenhouse above 0°C and below 10°C; so that the rootstock can pass through smoothly vernalization.

[0049] In this example, the rootstock variety is "Wei Luobo No. 1" provided by Weifang Academy of Agricultural Sciences, and the variety "Wei Luobo No. 1" is normally sold in the market.

[0050] In this embod...

Embodiment 2

[0078] A method for improving the efficiency of radish germplasm creation, specifically comprising the steps of:

[0079] S1. Preparation of rootstock and scion: choose "Weiluobo No. 1" (provided by Weifang Academy of Agricultural Sciences) with strong resistance and easy germination as the rootstock material; choose beautiful appearance, high quality, crisp green skin color, flesh color, spicy taste The light "Weibu 16" (provided by Weifang Academy of Agricultural Sciences) was used as scion material; the rootstock and scion seeds were sown in 50-hole seedling trays in mid-December and placed in the solar greenhouse; The method of covering with two films controls the greenhouse temperature above 0°C and below 10°C, so that the rootstock can pass vernalization smoothly.

[0080] S2. Planting: In the first ten days of March, plant the rootstock seed plants and scion seed plants in sheds when they are just about to bolt, with a row spacing of 1m and a plant spacing of 1m. When ...

Embodiment 3

[0102] A method for improving the efficiency of radish germplasm creation, specifically comprising the steps of:

[0103] S1. Preparation of rootstock and scion: choose "Weiluobo No. 1" (provided by Weifang Academy of Agricultural Sciences) with strong resistance and easy germination as the rootstock material; choose beautiful appearance, high quality, crisp green skin color, flesh color, spicy taste The light "Weibu 16" (provided by Weifang Academy of Agricultural Sciences) was used as scion material; the rootstock and scion seeds were sown in 50-hole seedling trays in mid-December and placed in the solar greenhouse; The method of covering with two films controls the greenhouse temperature above 0°C and below 10°C, so that the rootstock can pass vernalization smoothly.

[0104] S2. Planting: In the first ten days of March, plant the rootstock seed plants and scion seed plants in sheds when they are just about to bolt, with a row spacing of 1m and a plant spacing of 1m. When ...

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Abstract

The invention discloses a method for improving germplasm creation efficiency of radish, which comprises the following steps: S1, preparation of rootstocks and scions: selecting a hybrid variety easy to generate embryos as a rootstock material, selecting a hybrid variety with brittle green skin color and flesh color as a scion material, and sowing and vernalizing rootstock and scion seeds; S2, field planting; S3, grafting environment management: controlling the environment temperature to enable plants to grow, and irrigating roots with a chlormequat chloride diluent; S4, grafting time selection: taking the second and later primary branches of the rootstocks as grafting parts, and selecting primary branches with the length of 6-10 cm as the scions; S5, grafting: grafting the scion buds on rootstock plants; and S6, management after grafting: after grafting is completed, managing grafted plants by controlling the growth environment temperature. The method can effectively improve the embryogenesis rate of free microspores of the material difficult to embryogenesis, accelerate the purification speed of the radish material and improve the germplasm creation efficiency.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for improving the efficiency of radish germplasm creation. Background technique [0002] my country is one of the origins of radish (Raphanus sativus L.), and radish has a cultivation history of more than 2,700 years in China. Studies have shown that Shandong Luzhong, Luxi mountainous area and Huanghuai area are the origins of radish in my country; the perennial planting area in China About 18 million mu; my country's radish resources are relatively rich, there are more than 80 high-quality local varieties of fruit radish resources, and the distribution is relatively scattered, mainly in Shandong, Jiangsu, Hebei and other places. such as "Weixian radish" in Shandong, "Xinxinmei radish" in Beijing, and "Weiqing radish" in Tianjin. [0003] In recent years, with the improvement of people's living standards and the development of domestic and foreign situ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30
CPCA01G2/30
Inventor 孙继峰宋倩张静燕李萌韩鲁杰徐立功韩太利
Owner SHANDONG WEIFANG ACADEMY OF AGRICULTURAL SCIENCES
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