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Visual identification method for lily quality

An identification method, Baihe's technology, applied in the field of Baihe's quality visual recognition, can solve the problems of complex operation, difficulty in popularization, and long time consumption, and achieve the effect of broad application prospects, fast response, and portable device

Active Publication Date: 2022-01-21
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Due to the different water, soil, climate and other conditions in different producing areas, the quality of lilies is divided into good and bad, and the specific performance is that there are large differences in the types and contents of active compounds contained in them. , resulting in varying efficacy
At present, the common research on the quality of lily or other Chinese medicinal materials is generally achieved by chromatography, near-infrared spectroscopy, and other methods. These large-scale instrument methods are expensive, complicated to operate, and time-consuming. They can only be limited to laboratories and cannot be used by the general public. Accepted by the masses, it is difficult to popularize

Method used

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  • Visual identification method for lily quality
  • Visual identification method for lily quality
  • Visual identification method for lily quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A method for visual recognition of lily quality (lily origin), comprising the steps of:

[0045] 1) Nine different known origins (Hunan Lily No. 1, Hunan Longhui Longya Lily No. 2, Hunan Longshan Lily No. 3, Gansu Lanzhou Lily No. 4, Fujian Putian No. 5, 8-year-old Lanzhou Lily No. 6, Jiangxi Lily Fuzhou No. 7, Dian Lily No. 8, Jiangsu Lily No. 9, and Jiangxi Dabie Mountain No. 10; among them, Hunan Lily 1 was not marked with a more specific place of origin when purchased), and the lily samples were extracted to obtain the lily extract;

[0046] 2) Mix the obtained lily extract with the gold and silver nanocluster solution respectively, let it stand for 3 minutes to react, and observe the fluorescent color change of the lily extract before and after the reaction; the specific steps include:

[0047] a) Add 900 μL Tris-HCl buffer solution (pH=7.0) and 100 μL of the 10-fold dilution (diluted with water) of the lily extract prepared in step 1) to a 1.5 mL cuvette respectiv...

Embodiment 2

[0051] A method for visual recognition of lily quality (lily growth year), comprising the steps of:

[0052] 1) Four batches of lily samples of different years (1-year Lanzhou Qilihe, 2-year Lanzhou Qilihe, 3-year Lanzhou Qilihe, 8-year Lanzhou Lily) were extracted respectively;

[0053] 2) 100 μL of the 10-fold dilution (diluted with water) of the obtained lily extract was mixed with 100 μL of Tris-HCl buffer solution, and the concentration of 100 μL was 2×10 -6 The mol / L gold-silver nanocluster solution was reacted in a 96 microwell plate with a pore size of 400 μL for 2 min, and the fluorescent color change of the lily extract to be tested was observed before and after the reaction under the excitation light of 365 nm in an ultraviolet dark box; XR) Take photos of the fluorescent color change of lily before and after the reaction, the results are shown in image 3 a; then further use software to extract the RGB value of the corresponding photo and draw a fluorescent pictur...

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Abstract

The invention discloses a visual identification method for lily quality. The method comprises the following steps: uniformly mixing an extracting solution of a lily sample to be detected with a gold-silver nano-cluster, and preforming standing treatment for reaction; according to the color or fluorescence intensity information of the detection system obtained after the reaction and the color or fluorescence intensity response difference between the lily samples with different qualities, performing the rapid visual identification of the lily quality. Compared with existing detection methods based on chromatography and the like, the detection method has the advantages of being high in stability, rapid in response, easy and convenient to operate, portable in device and the like, has wide application prospects and can be put into industrial production.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a new method for visually identifying the quality of lilies. Background technique [0002] As a traditional medicine and food homologous product, lily is rich in polysaccharides, steroidal saponins and phenolic acids and other ingredients. Aging and enhancing the body's immunity and other medicinal effects. The health function and medicinal effect of lily are closely related to its quality. The excellent quality of lily is related to its suitable geographical environment and climatic conditions, and mature production and processing technology. The quality of lilies in different places of origin is quite different. For example, the authentic production areas of "medicinal lily" are Longhui, Longshan, and Xinshao in Hunan, while the original production areas of "edible lily" are Lanzhou, Gansu. Lilies from different origins are difficult to dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/64
CPCG01N21/78G01N21/643Y02P90/30G01N21/77G01N2021/7786G01N21/251G01N21/27
Inventor 付海燕韦柳娜王思语杨健陈亨业龙婉君杨小龙
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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