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Method for identifying radixstephaniae tetrandrae of different sources by FI-UV fingerprint spectrum

A FI-UV and fingerprint technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long time consumption, great energy consumption, long analysis time, etc.

Inactive Publication Date: 2019-12-31
安徽中医药高等专科学校
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Problems solved by technology

[0004] However, HPLC often requires a long analysis time. In the above-mentioned 3 documents, the fingerprint spectrum established by HPLC / UPLC technology often takes 20-50 minutes; in terms of quantification, chemical composition reference substances are required, and the cost is high; in large quantities The test does not have an advantage, it takes a long time and consumes a lot of physical strength

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  • Method for identifying radixstephaniae tetrandrae of different sources by FI-UV fingerprint spectrum
  • Method for identifying radixstephaniae tetrandrae of different sources by FI-UV fingerprint spectrum
  • Method for identifying radixstephaniae tetrandrae of different sources by FI-UV fingerprint spectrum

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Embodiment

[0027] The present invention utilizes FI-UV fingerprint atlas to identify different sources of Fangji method, and its identification method comprises the following steps:

[0028] S1. Preparation of Fangji for testing: use a pulverizer to pulverize Fangji from different sources; use an electronic analytical balance to weigh 1.00 g of pulverized Fangji powder, add 100 ml of aqueous methanol to the weighed Fangji powder, and use ultrasonic The extractor ultrasonically treated the obtained Fangji and methanol aqueous solution for 1 hour, then placed it at room temperature, made up for the lost weight, adjusted the volume to 100ml with methanol water, and filtered the obtained solution with a 0.45 μm organic filter membrane to obtain the Fangji test solution;

[0029] S2, the making of FI-UV fingerprint: Utilize liquid chromatograph to carry out chromatographic analysis to each Fangji for testing solution obtained respectively, and record the FI-UV fingerprint of each Fangji respec...

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Abstract

The invention provides a method for identifying radixstephaniae tetrandrae of different sources by FI-UV fingerprint spectrum. The identification method comprises the following steps of radixstephaniae tetrandrae test product preparation, FI-UV fingerprint spectrum fabrication, FI-UV fingerprint spectrum data pre-processing, chemometrics analysis and visual result identification. A high performance liquid chromatography (HPLC) technology is utilized, chromatographic column separation is not performed, only a protection column is used, rough division and prevention of pollution of plant constituents such as protein and polypeptide on a detector are only achieved, the rapid detection function is further achieved, the on-line scanning function of a diode array detector of liquid chromatography is utilized, on-line scanning is performed on chromatographic peak of a total elution curve, elution curve and scanning data thereof are obtained, rapid visual identification of radixstephaniae tetrandrae is achieved by combining of PCA and PLS-DA chemometrics means, and a person without professional background also can rapidly understand.

Description

technical field [0001] The invention relates to the field of identification of traditional Chinese medicines, in particular to a method for identifying Fangji from different sources by using FI-UV fingerprints. Background technique [0002] The modernization of traditional Chinese medicine is the theme of the development of traditional medicine in the 21st century. To realize the modernization of traditional Chinese medicine, we must first solve the problem of stable and controllable quality of traditional Chinese medicine. As we all know, traditional Chinese medicine belongs to a multi-component chemical system due to its various types of chemical composition structures and different components. The appearance and properties of medicinal materials may change with processing, making it difficult for traditional identification techniques to play a role, and traditional identification techniques have certain limitations and experience, and lack quantitative indicators. Based ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N2030/324
Inventor 秦亚东汪荣斌周娟娟张静文张梦雨
Owner 安徽中医药高等专科学校
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