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Biomarkers for ich prognostic assessment and their applications

A technology of exosomes and proteins, applied in the field of medical biology, can solve the problems of inability to meet clinical needs, high prognosis prediction value of ICH, and achieve the effect of improving clinical services

Active Publication Date: 2022-07-08
BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no recognized biomarker with high predictive value for the prognosis of ICH so far, which cannot meet the clinical needs

Method used

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  • Biomarkers for ich prognostic assessment and their applications
  • Biomarkers for ich prognostic assessment and their applications
  • Biomarkers for ich prognostic assessment and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Clinical data collection of ICH patients and healthy controls

[0066] This study included inpatients from Beijing Tiantan Hospital affiliated to Capital Medical University from October 2018 to November 2019 and patients in the multicenter China National Stroke Registry Study-III (CNSR-3). Inclusion criteria for patients with ICH: (1) the age of onset is between 18 and 80; (2) the patient is diagnosed with acute cerebral hemorrhage according to the clinical and imaging data of the patient; (3) the cause of the onset is hypertensive; (4) ) The bleeding location is around the basal ganglia, without breaking into the ventricle, and the bleeding volume is less than 50ml. Included clinical information includes: general basic information and clinical characteristics of patients, high-risk factors for large arterial atherosclerosis (hypertension, diabetes, coronary heart disease, hyperlipidemia, etc.), and treatment plans in the acute phase. Follow-up data includ...

Embodiment 2

[0067] Example 2: Sample collection and storage of ICH patients and healthy controls

[0068] 2.1 Collection of plasma---The peripheral blood was collected in a purple tube containing anticoagulant EDTA or heparin, centrifuged within 30min after sample collection, 3000rpm for 10min, 2-8℃. Upper plasma was collected and stored in batches at -80°C. Avoid repeated freezing and thawing of samples. Note: Samples should be centrifuged well to avoid hemolysis or the presence of particles.

[0069] 2.2 Extraction of exosomes from peripheral plasma

[0070] We use the Serum and Plasma ExoQuick Extracellular Vesicle Isolation Kit (Cat. No. EQULTRA-20A-1), an innovative kit with expertise in exosome isolation.

[0071] 2.2.1 Extraction

[0072] 1) Take out the sample, centrifuge at 3000g for 15min and take the supernatant.

[0073] 2) If there are still cell debris remaining, centrifuge the supernatant again at 12,000g for 10 min, and transfer the supernatant to a new centrifuge t...

Embodiment 3

[0100] Example 3: Gene and proteomic technology analysis, screening out differentially expressed proteins, and obtaining recurrence and prognosis assessment results. Detection combinations of markers

[0101] 3.1 This project uses next-generation label-free quantitative proteomics technology to complete the analysis. Under the data independent acquisition (DIA) mode, it can provide unparalleled proteomic coverage while achieving precise accuracy of a large number of proteins per sample , Highly reproducible quantification. The DIA workflow provides an ideal platform for qualitative analysis of differentially expressed proteomes or proteome quantification of massive samples. The DIA process is based on three necessary steps:

[0102] 1) Constructing a spectrum library: The spectrum library collects all detectable non-redundant high-quality peptide information (MS / MS spectrum) of the sample, which is used as a peptide identification template for subsequent data analysis. I...

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Abstract

The present disclosure provides a biomarker for ICH prognosis assessment and application thereof. Specifically, the present disclosure provides a reagent for detecting at least one peripheral plasma or exosomal protein, or at least one exosomal microRNA, in the preparation of a reagent for predicting, diagnosing or monitoring transient ischemic attack or application in the kit. The technical solutions provided by the present disclosure form a new biomarker panel that helps to better understand the pathophysiology of ICH, and will provide new opportunities for diagnosis and prognosis, thereby improving clinical services for ICH patients.

Description

technical field [0001] The present disclosure relates to the field of medical biology. In particular, the present disclosure relates to biomarkers, and their use in diagnosing brain injury or brain-related injury, particularly in the diagnosis of intracerebral hemorrhage (ICH), and to the use of biomarkers in individuals for Methods of detecting, predicting or monitoring cerebral hemorrhage. Background technique [0002] Intracerebral hemorrhage (ICH) refers to hemorrhage caused by rupture of blood vessels in the non-traumatic brain parenchyma, accounting for 20% to 30% of all strokes, and the acute mortality rate is 30% to 40%. The cause is mainly related to cerebrovascular lesions, that is, it is closely related to hyperlipidemia, diabetes, hypertension, vascular aging, and smoking. Patients with cerebral hemorrhage often develop sudden onset due to emotional agitation and exertion, and early mortality is very high. After ICH, hematoma decomposes and releases a variety ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68C12Q1/6883
CPCG01N33/6893C12Q1/6883C12Q2600/118C12Q2600/158C12Q2600/178G01N2800/2871G01N2800/52G01N2800/60
Inventor 施福东王拥军金薇娜
Owner BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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