Culture medium for rejuvenating edible fungus strain and method for rejuvenating edible fungus strain

Abstract

The invention relates to a culture medium for rejuvenating an edible mushroom strain and a method for rejuvenating the edible mushroom strain. The culture medium comprises a water agar culture medium and a wood strip, and the wood strip is selected from xylem; the method comprises the following steps: preparing the water agar culture medium and the wood strip; and inoculating the strain on the water agar culture medium, placing the wood strip in the water agar culture medium, and then culturing. The culture medium disclosed by the invention can be used for rejuvenating edible mushroom strains, so that the growth speed of edible mushrooms can be increased; the method is clear in target directivity, has the advantages of being simple to operate, good in rejuvenation effect and the like, can be widely applied to rejuvenation of edible mushroom strains produced on a large scale, and improves production efficiency and production benefits.

Description

Technical field

[0001] The present invention is in the field of agricultural biotechnology, particularly to a method used in edible fungi rejuvenation Culture media and edible fungi rejuvenation. Background technique

[0002] Artificial cultivation process, edible prone to a fading phenomenon, specific performance degradation mushroom species are: expected to eat slowly after inoculation mycelium, mycelium growth is slow, dense white colonies appeared on the fan culture; adverse external environment weak immune system, bacteria are vulnerable to infection, and even turn color anomaly; fruiting body formation is often delayed, cap, stipe malformations; fruiting tide times not obvious, occurs less, low mushroom production, poor quality; mushrooms mycelium become thin, slow growth, early fruiting culture of material culture is easy, easy to individual small parachute, mushroom wood-disintegration, short fruiting life.

[0003] Therefore, to establish a convenient and efficient metho...

Examples

Embodiment 1

[0036] 1) in a test tube was added 2ml of water agar (15g agar, 1000ml deionized water), placed vertically, autoclaving, sterilization temperature 115 deg.] C, sterilization time 15min, the test tube is placed vertically sterilized cool down.

[0037] 2) The specifications of 6cm × 0.5cm × 0.3cm hardwood strips soaked in deionized water three days, and then sterilized under conditions of 126 deg.] C 120min.

[0038] 3) Inoculation of mushroom strains on the cooled agar medium, then insert the sterilized hardwood wood inserted into the agar medium, place the test tube into the incubator (25 ° C) vertically.

[0039] 4) Through the bacterium to 3-4cm on the wood strip, starting a continuous passage training on the wooden strip.

Embodiment 2

[0052] The difference from Example 1 is only that the hardwood wood is replaced with a cork wood strip of the same specification.

Technical field

[0001] The present invention is in the field of agricultural biotechnology, particularly to a method used in edible fungi rejuvenation Culture media and edible fungi rejuvenation. Background technique

[0002] Artificial cultivation process, edible prone to a fading phenomenon, specific performance degradation mushroom species are: expected to eat slowly after inoculation mycelium, mycelium growth is slow, dense white colonies appeared on the fan culture; adverse external environment weak immune system, bacteria are vulnerable to infection, and even turn color anomaly; fruiting body formation is often delayed, cap, stipe malformations; fruiting tide times not obvious, occurs less, low mushroom production, poor quality; mushrooms mycelium become thin, slow growth, early fruiting culture of material culture is easy, easy to individual small parachute, mushroom wood-disintegration, short fruiting life.

[0003] Therefore, to establish a convenient and efficient metho...

Examples

Embodiment 1

[0036] 1) in a test tube was added 2ml of water agar (15g agar, 1000ml deionized water), placed vertically, autoclaving, sterilization temperature 115 deg.] C, sterilization time 15min, the test tube is placed vertically sterilized cool down.

[0037] 2) The specifications of 6cm × 0.5cm × 0.3cm hardwood strips soaked in deionized water three days, and then sterilized under conditions of 126 deg.] C 120min.

[0038] 3) Inoculation of mushroom strains on the cooled agar medium, then insert the sterilized hardwood wood inserted into the agar medium, place the test tube into the incubator (25 ° C) vertically.

[0039] 4) Through the bacterium to 3-4cm on the wood strip, starting a continuous passage training on the wooden strip.

Embodiment 2

[0052] The difference from Example 1 is only that the hardwood wood is replaced with a cork wood strip of the same specification.

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