Cell electrochemistry and electrophysiology combined detection device and method

Abstract

The embodiment of the invention discloses a cell electrochemistry and electrophysiology combined detection device and method. The device comprises a detection electrode, a patch clamp amplifier, a filter module and a control and analysis module, wherein the detection electrode is used for detecting an electric signal and comprises an electrophysiological electrode and an electrochemical electrode; the patch clamp amplifier is used for amplifying the electric signal and comprises an electrophysiological detection unit connected with the electrophysiological electrode and an electrochemical detection unit connected with the electrochemical electrode; the filter module is used for filtering the electric signal and comprises a first band elimination filter, a second band elimination filter and a low-pass filter; and the control and analysis module is used for analyzing the electrophysiological condition and the electrochemical condition of the cell based on the electric signal, and is also used for sending a control signal to the patch clamp amplifier so as to adjust the detection mode of the patch clamp amplifier. According to the embodiment of the invention, cell electrophysiological signals can be detected, contents in living cells can be detected, electrophysiological and electrochemical detection capabilities are realized, the detection electrode does not need to be probed into the cells, the requirement on the detection electrode is lower, and the detection range is wider.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of biological detection, and in particular to a cell electrochemical and electrophysiological combined detection device and detection method. Background technique [0002] Cells contain a large number of chemical components with different functions, including water, sugars, inorganic substances, free neurotransmitters, and cell metabolites. Vesicles are the carriers for storing and exchanging information substances in cells. Under certain circumstances, they are released by fusion and diffused to the surface of another cell to realize the transmission of information. Therefore, detecting the contents of vesicles is of great significance for studying the way of information exchange between cells. The detection of chemical substances contained in cells, especially the information substances contained in vesicles with a size of only nanometers, usually requires electrochemical means. ...

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Problems solved by technology

However, this method uses nanoscale tip carbon fiber electrodes to directly penetrate cells, and there are still many difficulties in terms of technology and application: the penetration recording mode is mainly used for large-volume cultured cells that are easy to observe and distinguish, which means This means that it is difficult to achieve relevant electrochemical recordings at the level of brain slices that are closer to the physiological state or under in vivo conditions; the penetrating recording mode requires extremely thin carbon fiber tips, which limits the application of coating modification

Therefore, this method is only suitable for the inspection of individual (higher electrochemically active) transmitters that can directly react with carbon fiber electrodes, while for other neurotransmitters such as glutamic acid, specific enzyme coating of carbon fiber electrodes is required. The composition of the electrochemical detection can only be realized after the layer modification (thickness is usually micron), and the penetrating recording mode is difficult to realize; there have been published articles on piercing the electrode after the surface modification enzyme into a large volume of toad oocytes , but the reference electrode needs to be inserted at the same time for detection, which not only causes great damage to the cells, but also cannot detect electrical signals at the same time

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