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Compound microbial agent and application thereof in shrimp intestinal micro-ecological regulation

A technology of compound microbial inoculants and bacilli, applied in the directions of microorganism-based methods, microorganisms, applications, etc., can solve the problems of unsecured and damaged food safety, increase the activity of macrophages, improve the ecological balance, and increase the antibody the effect of

Pending Publication Date: 2022-02-08
深圳市贝翡尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it can speed up the growth of aquatic products, the entry of these hormones into the human body will cause certain damage to various organs of the human body, making food safety unsafe

Method used

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  • Compound microbial agent and application thereof in shrimp intestinal micro-ecological regulation
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  • Compound microbial agent and application thereof in shrimp intestinal micro-ecological regulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: a composite microbial inoculum comprising Bacillus cereus, Bacillus yellow sea, Bacillus amyloliquefaciens, Lactobacillus brucei, Bacillus subtilis, Brevibacterium acetyl and Lactobacillus maize;

[0023] The Bacillus cereus, the Bacillus yellow sea, the Bacillus amyloliquefaciens, the Lactobacillus brucei, the Bacillus subtilis, the Brevibacterium acetylide and the Lactobacillus maize are at a ratio of 2:0.5:4 : The weight composition ratio of 0.5:2:1:3 is mixed evenly;

[0024] The preparation method of the composite microbial inoculum is as follows: Bacillus cereus, Bacillus yellow sea, Bacillus amyloliquefaciens, Lactobacillus brucelli, Bacillus subtilis, Brevibacterium acetyl and Lactobacillus maize are respectively purified and inoculated into the culture medium Fermentation, when the OD value of the bacterial liquid is 0.6, and then according to the medium inoculation amount of 5%, inoculate and shake to obtain a single bacterial liquid; Liquid, La...

Embodiment 2

[0035] Embodiment 2: a kind of composite microbial inoculum, the difference from embodiment 1 is:

[0036] The Bacillus cereus, the Bacillus yellow sea, the Bacillus amyloliquefaciens, the Lactobacillus brucei, the Bacillus subtilis, the Brevibacterium acetylide and the Lactobacillus maize are in a ratio of 4:1.0:5 : The weight composition ratio of 1.0:4:3:4 is evenly mixed;

[0037] When the OD value of the obtained bacterial liquid is 0.7;

[0038] The number of active bacterial species in the composite microbial inoculum is 3.0×10 10 cfu / mL.

[0039] The mixing ratio of the compound microbial inoculum and the common feed is 1:4.

Embodiment 3

[0040] Embodiment 3: a kind of composite microbial inoculum, the difference from embodiment 1 is:

[0041] The Bacillus cereus, the Bacillus yellow sea, the Bacillus amyloliquefaciens, the Lactobacillus brucelli, the Bacillus subtilis, the Brevibacterium acetylide and the Lactobacillus maize are according to 6:1.2:7 : The weight composition ratio of 1.2:6:5:6 is evenly mixed;

[0042] The OD value of the obtained bacterial solution is 0.8.

[0043] The number of active bacterial species in the composite microbial inoculum is 5.0×10 10 cfu / mL.

[0044] The mixing ratio of the compound microbial inoculum and the common feed is 2:7.

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Abstract

The invention discloses a compound microbial agent and an application thereof in shrimp intestinal micro-ecological regulation. The compound microbial agent comprises bacillus cereus, Bacillus marisflavi, bacillus amyloliquefaciens, lactobacillus buchneri, bacillus subtilis, brevibacterium acetyliforme and lactobacillus zeae. The bacillus cereus, Bacillus marisflavi, bacillus amyloliquefaciens, lactobacillus buchneri, bacillus subtilis, brevibacterium acetyliforme and lactobacillus zeae are uniformly mixed according to a weight ratio of (2-6):(0.5-1.2):(4-7):(0.5-1.2):(2-6):(1-5):(3-6). The microbial preparation is an aqueous solution, provides a good growth environment and necessary nutrient substances for microorganisms, is beneficial to growth and metabolism of the microorganisms, contains a large number of viable bacteria, and can play a better role.

Description

technical field [0001] The invention belongs to the technical field of environmental treatment and treatment, and in particular relates to a composite microbial inoculum for regulating the microecology of shrimp intestinal tract. Background technique [0002] China is a big fishery country and also a big aquaculture country. It is the only fishery country in the world whose aquaculture output exceeds fishing output. Aquaculture products have become the main source of China's aquatic product supply. From 1949, when the People's Republic of China was founded, to 2012, the output of aquatic products in China increased from more than 1 million tons to more than 59 million tons. Among them, the proportion of aquaculture in the total aquatic products increased from 8% to 72.67%. China's aquaculture production has also accounted for about 70% of the world's aquaculture production. [0003] In the process of traditional aquaculture, in order to accelerate the growth rate of the aqu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/80A23K10/18C12R1/085C12R1/07C12R1/125C12R1/225C12R1/13
CPCC12N1/20A23K50/80A23K10/18A23V2400/187A23V2400/11Y02A40/818
Inventor 林大淇
Owner 深圳市贝翡尔科技有限公司
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