Application of senescence-related differential expression gene in preparation of cell senescence diagnostic kit

A technology for differentially expressed genes and diagnostic kits, which can be applied in the field of biomedicine and can solve the problem that the contact report has not been reported yet.

Pending Publication Date: 2022-02-11
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

IRS2 is a factor that plays an important role in the occurrence and treatment of tumors, and it has also been reported to play a role in neurodegenerative diseases, but the link between their m6A modification and aging has not been reported

Method used

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  • Application of senescence-related differential expression gene in preparation of cell senescence diagnostic kit
  • Application of senescence-related differential expression gene in preparation of cell senescence diagnostic kit
  • Application of senescence-related differential expression gene in preparation of cell senescence diagnostic kit

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Experimental program
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Effect test

Embodiment 1

[0054] (1) Cell culture

[0055] The cells were placed at a temperature of 37°C, a relative humidity of 95%, and CO 2 Aseptic culture in a cell culture incubator with a volume fraction of 5%. The culture medium is L-DMEM low-glucose medium. When the cell confluency reached 90%, the cells were subcultured (1:2, 1:3 or 1:4) according to actual needs, and the cells were counted. The calculation formula of population doubling levels (PDL) is n=3.32(logN2-logN1)+X, where n is the final PDL of the subcultured cells, N2 is the total number of cells harvested from this generation of cells, and N1 is the inoculation of the previous generation The number of cells, X is the PDL of the previous generation of cells.

[0056] (2) Progeria cell model and replicative senescence cell model

[0057] A cell model of progeria: using the young cell group 22PDL for H 2 o 2 Infection, the same number of 22PDL was inoculated into the cell culture flask (1:3 passaging), and when the cells grew t...

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Abstract

The invention discloses application of a senescence-related differential expression gene in preparation of a cell senescence diagnostic kit, and belongs to the technical field of biomedicine. Through screening and verification, the protein expression level and m6A RNA methylation modification level of the senescence-related differential expression genes SIRT3, E2F3 and IRS2 are found for the first time, the method can be used for identifying and diagnosing the senescence state of cells, the problem that diagnostic markers in the field of cell senescence are in shortage at present is solved, and a reference basis is provided for early prevention and targeted therapy of cell premature senility and age-related diseases.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of aging-related differentially expressed genes in the preparation of a cell aging diagnostic kit. Background technique [0002] Cellular senescence is a permanent state of cell cycle arrest. Senescent cells will accumulate as the body ages, releasing a series of pro-inflammatory and pro-apoptotic factors, leading to the occurrence of age-related diseases and chronic diseases, such as atherosclerosis, type 2 diabetes, arthritis, idiopathic pulmonary fibrosis and liver disease. Therefore, the identification, characterization, and pharmacological elimination of senescent cells has received attention in the field of aging research. [0003] Cellular senescence is the basis of organ and individual aging, and its process is affected by many complex factors such as genetics and environment. For a long time, the scientific community has not yet clarifie...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N33/68C12Q1/6888C12Q1/6883
CPCG01N33/6893C12Q1/6888C12Q1/6883C12Q2600/154G01N2800/60G01N2800/7042
Inventor张文娟林辉张文姬吴帆彭欣悦余素素周铖张露云
OwnerJINAN UNIVERSITY