Method for verifying influence of Orius sauteri Poppius on activity of endogenous substances in soybean aphid bodies under stress

Abstract

The invention relates to a method for verifying the influence of Orius sauteri Poppius on the activity of endogenous substances in soybean aphid bodies under stress, and belongs to the technical field of agricultural science. The method is characterized in that: compared with soybean aphids at a same age in a control group that are not stressed, soybean aphids at the age of 1-4 instar being stressed by Orius sauteri Poppius have the characteristics that the specific activity of acetylcholin esterase and carboxylic ester hydrolase in vivo is remarkably improved and reaches a maximum value at the age 1 instar; the specific activity of glutathione-S-transferase of the stressed soybean aphids of 3<rd>-5 instar is lower than that of a treated group, the specific activity of the stressed soybean aphids of 1-2<nd> instar is obviously higher than that of the control group, and the specific activity of the soybean aphids of 2<nd> instar reaches a maximum value; the total protein content of the stressed 1-4 instar soybean aphids is obviously lower than that of a control group, and the stressed last-instar soybean aphids have no obvious difference from the control group. The conclusion is that stress of Orius sauteri Poppius has obvious influence on the content of various biochemical substances in bodies of low-instar soybean aphids, and has little influence on the content of biochemical substances in bodies of last-instar soybean aphids.

Description

technical field [0001] The invention relates to a method for verifying that the stress of the oriental stinkbug affects the activity of endogenous substances in soybean aphids, and belongs to the field of agricultural science and technology. Background technique [0002]Orius sauteri belongs to the family Hemiptera and is one of the important predatory natural enemy insects in northern my country. It has the characteristics of early occurrence, long time, wide distribution, large number, mixed feeding habits and strong activity Yang Shufei et al., 2009). It mainly feeds on small insects such as thrips, aphids, spider mites, and whiteflies, plant pollen, and eggs and small larvae of noctuid insects (Nagayi K, 1997). The occurrence of insects plays an important role and has become the main species exploited and utilized by insect natural enemies. Domestic studies have been carried out on the biological characteristics, occurrence, growth and decline, activity rules and artifi...

AI Technical Summary

Problems solved by technology

Therefore, after the stress of japonica, the respiration in the soybean aphid is strengthened, and the substrates that need to undergo glycolysis need to be greatly increased for the consumption of respiration. Therefore, after the stress, the content of reducing sugar in the soybean aphid increases greatly. However, although the content of reducing sugar in the adult aphid stage increased slightly, there was no significant difference at the 0.05 level. There is a significant difference at the 0.01 level, which shows that the stress of the East Asian flower bug has a very significant impact on the young soybean aphid

Images