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Method for inhibiting geminivirus infection by using arabidopsis ABI5 protein overexpression

A gemini virus, overexpression technology, applied in biochemical equipment and methods, viruses/phages, chemical instruments and methods, etc., can solve problems such as plant cell stress, plant dwarf phenotype, plant stress effects, etc., and achieve inhibition of viral transcription. and infestation, increasing resistance, avoiding effects

Active Publication Date: 2022-03-01
SOUTH CHINA NORMAL UNIVERSITY
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Problems solved by technology

[0006]The main problem of this technology is: the overexpression of LSB1 / GDU3 gene itself constitutively activates the immune pathway of plants, affects the plant development under normal growth conditions, and affects the The plant itself causes the stress effect, leading to the dwarf phenotype of the plant; in addition, the mechanism of this method’s anti-virus action is unclear, which may lead to unknown side effects
[0008]The main problem of this technology is: it is necessary to apply AtPep1 small peptide while overexpressing PEPR2, which increases the difficulty and expense of antiviral implementation; at the same time, this method is harmful to plants Activation of immune pathways may exert general stress effects on plant cells

Method used

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  • Method for inhibiting geminivirus infection by using arabidopsis ABI5 protein overexpression
  • Method for inhibiting geminivirus infection by using arabidopsis ABI5 protein overexpression
  • Method for inhibiting geminivirus infection by using arabidopsis ABI5 protein overexpression

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Embodiment

[0028] Such as figure 1 As shown, ABI5 can specifically bind to the promoter of BSCTV.

[0029] In order to find a plant protein that specifically binds to the BSCTV promoter, the applicant used the BSCTV genome (ATCCPVMC-6; formerly known as the BCTV-CFH strain, which stored the double-stranded DNA of BSCTV linearized with EcoRI on the plasmid pCFH) as Template, by PCR amplification of BSCTV cis promoter Pwt (1-356 bp region of BSCTV genome, the specific sequence is as follows Figure 4 As shown, the primer information is as follows:

[0030] Pwt-F: 5'-AGTAAGCTTATTGAATCGGGCTCTCTTCAAATC-3';

[0031] Pwt-R: 5'-AGTGTCGACATTTATAAGTACATATACATGTAA-3'), constructed into the pABAi plasmid to obtain the bait plasmid pABAi-Pwt. Then, the bait plasmid was used to transform the yeast strain (Y1HGold) together with the cDNA library of Arabidopsis thaliana constructed on pGADT7, and the Arabidopsis protein that interacted with Pwt was screened by yeast single hybridization. ABI5 protei...

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Abstract

The invention discloses a method for inhibiting geminivirus infection by overexpression of arabidopsis ABI5 protein, which inhibits transcription of geminivirus coding genes by overexpression of plant transcription factor ABI5 protein and combination of ABI5 protein and a promoter of geminivirus through specificity, and realizes improvement of virus resistance of plants.

Description

technical field [0001] The invention relates to the technical field of plant control, in particular to a method for suppressing geminivirus infection by using the overexpression of Arabidopsis ABI5 protein. Background technique [0002] Geminivirus (Geminivirus) is an important class of plant viruses with a single-stranded DNA genome, which poses a serious threat to agricultural production. Because geminiviruses have caused great harm to the yield and quality of food crops and economic crops in my country, and the diseases caused by them are increasing year by year, so the prevention and control of this family of viruses is of great significance to ensure the safety of agricultural production. [0003] The genome of geminiviruses undergoes massive replication in the plant cell nucleus, and then produces its encoded proteins through transcription and translation. These encoded viral proteins are used to interact with host cells, thereby completing its life cycle in plant cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/84A01H5/00A01H6/82A01H6/20
CPCC07K14/415C12N15/8283C12N2830/34
Inventor 赖建彬阳成伟黄微高彩吉
Owner SOUTH CHINA NORMAL UNIVERSITY
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