Test method for increasing serum testosterone of adult rat by resetting pituitary steady state through bisphenol A exposure

A technology of serum testosterone and testing methods, applied in biological testing, material analysis through optical means, and analysis through chemical reactions of materials, etc., can solve the problem of lack of good judgment research methods and unclear mechanism of BPA long-term impact research And other issues

Active Publication Date: 2022-03-04
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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Problems solved by technology

However, at this stage, the long-term effects of BPA exposure and the research mechanism are still unclear, and the research methods are not well judged.

Method used

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  • Test method for increasing serum testosterone of adult rat by resetting pituitary steady state through bisphenol A exposure
  • Test method for increasing serum testosterone of adult rat by resetting pituitary steady state through bisphenol A exposure
  • Test method for increasing serum testosterone of adult rat by resetting pituitary steady state through bisphenol A exposure

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Embodiment Construction

[0064] An embodiment of the test method for increasing adult rat serum testosterone by resetting the pituitary gland homeostasis through bisphenol A exposure of the present invention: comprising the following steps,

[0065] Step 1, SD rats were placed in a constant temperature environment of 23±2°C and 55±5% constant humidity for 12 hours in a light / dark cycle, and SD rats were allowed to obtain food and water freely, and BPA was dissolved in Diluted in ethanol, and then diluted in tocopherol-exfoliated corn oil until the final concentration of ethanol was 0.5%, for intragastric administration; SD rats were randomly divided into three groups, which were set as the control group, low-dose group and high-dose group respectively. Dose group, 8 rats in each group, from the 21st day after birth, 0, 0.1 or 10mg / kg BW dosage of BPA was administered orally every day, and continued for 14 days; the SD rats were killed on the 56th day after birth; the dried blood was collected, Centrif...

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Abstract

The invention discloses a test method for increasing serum testosterone of an adult rat by resetting pituitary steady state through bisphenol A exposure. Comprising the following steps: culturing an SD rat and obtaining testis, measuring T level, extracting total RNA, purifying and synthesizing cDNA, carrying out qPCR reaction, obtaining a total protein sample, carrying out Western protein band density analysis, slicing and dyeing the testis, carrying out transcriptome sequencing analysis, analyzing functions of differential expression genes (DEGs), calculating and comparing gene percentages of different testis cell types, and the like. The method can be used for researching and judging the influence mechanism of bisphenol A on testicular interstitial cell development, and the steps are simple and efficient.

Description

technical field [0001] The invention relates to the technical field of biomedicine. Background technique [0002] In 1981, bisphenol A, or 2,2-bis(4-hydroxyphenyl)propane, abbreviated as BPA, was synthesized for the first time as a monomer for the manufacture of polycarbonate plastics. Since then, it has been used in a wide variety of consumer products, including those that come in close contact with food, such as plastic baby bottles, food jar liners and dental restoration materials. In addition to potential exposure from the general environment, namely water, air and soil, BPA may also enter the human body directly through contaminated food. Surveys have shown that BPA can be detected in urine samples of 90% of the general population in the United States, with mean serum concentrations of 2.5-10.95 ng / ml. Meanwhile, a recent study found that the total bisphenol content in the urine of Chinese children aged 3 to 11 ranged from 0.43 to 31.5 ng / ml. [0003] At present, stu...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/53G01N33/533G01N21/64G01N21/76
CPCG01N33/74G01N33/53G01N33/533G01N21/6428G01N21/76G01N2021/6432
Inventor 陈浩林陈丹管小菊赵星仪黄福
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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