Application of astaxanthin in promoting development of preantral follicles of mice and preparation

A technology of preantral follicles and astaxanthin is applied in the application and preparation field of astaxanthin in promoting the development of preantral follicles in mice, so as to promote in vitro development, alleviate oxidative damage, improve development rate and secretion of estrogen Effect

Active Publication Date: 2022-03-11
JINLIN MEDICAL COLLEGE
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, few studies have applied astaxanthin to the in vitro culture system of pr

Method used

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  • Application of astaxanthin in promoting development of preantral follicles of mice and preparation
  • Application of astaxanthin in promoting development of preantral follicles of mice and preparation
  • Application of astaxanthin in promoting development of preantral follicles of mice and preparation

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Embodiment

[0023] 1 Materials and methods

[0024] 1.1 Experimental animals

[0025] Sixty female clean-grade Kunming mice on the 14th day of birth were purchased from Changchun Yisi Experimental Animal Center. The mice were kept in an environment with a room temperature of (22±2)°C and a humidity of (50±10)%. The light cycle was maintained at 14h light+10h dark, and the mice were free to eat and drink. This experiment was reviewed and approved by the Medical Research Ethics Committee of Jilin Medical University, and the experimental operation complied with the regulations on the management of animal experimental research.

[0026] 1.2 In vitro culture of preantral follicles

[0027] The mice were sacrificed by cervical dislocation, and their bilateral ovaries were quickly removed and placed in L-15 working solution (Gibco, USA, 11415064) containing 10% fetal bovine serum (FBS). The tissue around the ovary is separated and removed under a stereoscope, and the follicles are separated u...

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Abstract

The invention relates to application of astaxanthin, in particular to application of astaxanthin in promoting development of preantral follicles of mice and a preparation. The astaxanthin is added into the in-vitro culture solution of the preantral follicles of the mouse to explore the antioxidant effect and the antioxidant mechanism of the preantral follicles in the follicle development process, and the result shows that the astaxanthin reduces lipid peroxidation in the follicles by improving the expression of antioxidant genes in the follicles and improves the follicle development rate and estrogen secretion, so that the anti-oxidation effect of the follicles is improved. The in-vitro development of the preantral follicles of the mice is promoted, and the result provides reference for the application in the in-vitro culture of follicles of other species.

Description

technical field [0001] The invention relates to the application of astaxanthin, in particular to the application and preparation of astaxanthin in promoting the development of mouse preantral follicles. Background technique [0002] Follicle development is a key and complex biological process to maintain human reproduction. At present, the in vitro culture technology of primordial follicles and preantral follicles, and the in vitro maturation technology of cumulus-oocyte complexes (CoCs) have become important research methods for the study of follicle development and oocyte maturation mechanisms. Mother cells are also an important source of mature oocytes in clinical assisted reproductive technology. In order to improve the in vitro culture effect of the above-mentioned technical system, people have added hormones, growth factors and some plant extracts to the culture medium to promote follicle development and oocyte maturation in vitro. In the process of follicle culture ...

Claims

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Application Information

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IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2501/999C12N2501/31C12N2500/25Y02A40/81
Inventor 潘晓燕刘丽梅李亚秋刘思彤徐妲冯磊张万生王雪楠
Owner JINLIN MEDICAL COLLEGE
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