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A kind of breeding method of male and female pigeons with long and short cashmere

A technology of self-sex separation and breeding method, which is applied to the cultivation of long and short cashmere self-sex male and female pigeons, and the field of pigeon breeding, can solve the problems of low success rate of self-sex separation, general egg production performance, and inability to separate male and female offspring, etc., to achieve The effect of cost reduction, important breeding value and economic value, and wide range of variety adaptation

Active Publication Date: 2022-05-27
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, the Teikesen pigeon strain has a large body size and average egg production performance, so it is not suitable as a breeding material for small grain-saving egg pigeons. In addition, the feather color of this strain has strict requirements on the matching method of the parent generation's feather color. Compared with other breeds When crossbreeding, the success rate of self-separation will be reduced, especially when using Texen pigeons to breed egg pigeons, because the genetic background of white feather color is very complicated, and its genetic law is not clear at present, so it cannot be introduced into the current market. The high-yield white feather breeds are crossed with it, otherwise it will destroy the original feather color self-identification mode of Texen pigeons, resulting in the inability of the offspring to distinguish between male and female

Method used

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  • A kind of breeding method of male and female pigeons with long and short cashmere
  • A kind of breeding method of male and female pigeons with long and short cashmere

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Embodiment 1

[0018] A method of breeding from separate male and female pigeons, such as figure 2 shown, including the following steps:

[0019] Step 1. Select 500 adult silver king pigeons from Nanjing Dongchen Pigeon Industry Co., Ltd. as the preselected group, wear foot rings, collect blood from the lower wing veins, extract DNA, and analyze the 769369bp locus containing the Z chromosome (refer to the genome version Cliv_1.0 ) fragment was amplified by PCR, upstream primer F: 5'-GATGCTGAGGCCTTTCATGT-3' (as shown in SEQ ID NO: 1) downstream primer R: 5'-TTTCCCCAAAGATCCAACAG-3' (as shown in SEQ ID NO: 2) , the final concentration of the reaction system (50 μl) is: 50 ng of pigeon DNA to be tested, 1.25 IU of Accurate Taq DNA polymerase, 5 μl of 10X PCR reaction buffer (containing Mg2+), 1 μl of 10 mM dNTPs, 1 μl of 10 μM upstream primer F, 10 μM downstream primer R 1 μl, make up to 50 μl with sterile water. The reaction conditions of PCR amplification were: pre-denaturation at 94°C for ...

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Abstract

The invention relates to a method for breeding pigeons, in particular to a method for breeding male and female pigeons with long and short cashmere, and belongs to the field of biotechnology. The invention discloses a method for breeding pigeons that can be separated from male and female. It includes identifying the genotype of the natal-down gene locus on the Z chromosome of the breeding pigeon that controls the long and short down traits of young down feathers, and breeding long-down pure lines and short-down pure lines. The male and female pigeons of different genotypes are used as the male and female parents to crossbreed according to a certain combination to produce a commercial generation. The commercial generation can be based on the long-down and short-down phenotypes of the young pigeons in the first 5 days after hatching to identify males and females. The method provided by the present invention greatly reduces the cost of early gender identification in the process of breeding traditional pigeon commercial generations. At the same time, compared with the existing feather color self-identification method, the method is not restricted by the strict matching of the feather color of the parents, and adapts to The range is wider, and the breeding material can be selected more flexibly to cultivate pigeon matching lines, which has important breeding value and economic value.

Description

technical field [0001] The invention relates to a method for cultivating pigeons, in particular to a method for cultivating long-short-velvet pigeons from male and female pigeons, belonging to the field of biotechnology. Background technique [0002] Pigeons are the fourth largest poultry in my country after chickens, ducks and geese. By 2021, the number of pigeons in the country has exceeded 80 million pairs. At present, the main feather color strains in the production of egg pigeons in my country are American king pigeon, Kanu pigeon, Texon pigeon, Texen hybrid derivative varieties, etc. Among them, white-feathered pigeons such as white-feather king pigeon and white Kanu pigeon have better egg-laying performance. It is the main species cultivated in my country. At present, one of the bottlenecks restricting the development of my country's pigeon industry is the early sex identification of pigeons. As a late adult bird, the pigeon weighs about 20g after hatching, and its p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
Inventor 冯春刚段颖史凯李东锋赵茜宋迟徐善金
Owner NANJING AGRICULTURAL UNIVERSITY
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