A kind of breeding method of male and female pigeons with long and short cashmere
A technology of self-sex separation and breeding method, which is applied to the cultivation of long and short cashmere self-sex male and female pigeons, and the field of pigeon breeding, can solve the problems of low success rate of self-sex separation, general egg production performance, and inability to separate male and female offspring, etc., to achieve The effect of cost reduction, important breeding value and economic value, and wide range of variety adaptation
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[0018] A method of breeding from separate male and female pigeons, such as figure 2 shown, including the following steps:
[0019] Step 1. Select 500 adult silver king pigeons from Nanjing Dongchen Pigeon Industry Co., Ltd. as the preselected group, wear foot rings, collect blood from the lower wing veins, extract DNA, and analyze the 769369bp locus containing the Z chromosome (refer to the genome version Cliv_1.0 ) fragment was amplified by PCR, upstream primer F: 5'-GATGCTGAGGCCTTTCATGT-3' (as shown in SEQ ID NO: 1) downstream primer R: 5'-TTTCCCCAAAGATCCAACAG-3' (as shown in SEQ ID NO: 2) , the final concentration of the reaction system (50 μl) is: 50 ng of pigeon DNA to be tested, 1.25 IU of Accurate Taq DNA polymerase, 5 μl of 10X PCR reaction buffer (containing Mg2+), 1 μl of 10 mM dNTPs, 1 μl of 10 μM upstream primer F, 10 μM downstream primer R 1 μl, make up to 50 μl with sterile water. The reaction conditions of PCR amplification were: pre-denaturation at 94°C for ...
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