Method for breeding long and short velvet auto-sexing pigeons
A technology of self-sex separation and breeding method, applied in the biological field, can solve the problems of low success rate of self-sex separation, general egg production performance, inability to separate sexes of offspring, etc., and achieve cost reduction, important breeding value and economic value, variety Adapt to a wide range of effects
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[0018] A kind of breeding method of self-separating male and female pigeons, such as figure 2 shown, including the following steps:
[0019] Step 1. Select 500 adult silver king pigeons from Nanjing Dongchen Pigeon Industry Co., Ltd. as a pre-selected group, wear upper leg rings, take blood from the vein under the wings, and extract DNA. The 769369bp site containing the Z chromosome (reference genome version Cliv_1.0 ) for PCR amplification, upstream primer F: 5'-GATGCTGAGGCCTTTCATGT-3' (as shown in SEQ ID NO: 1) and downstream primer R: 5'-TTTCCCCAAAGATCCAACAG-3' (as shown in SEQ ID NO: 2) , the final concentration of the reaction system (50 μl) is: 50 ng of pigeon DNA to be tested, 1.25 IU of Accurate Taq DNA polymerase, 5 μl of 10X PCR reaction buffer (containing Mg2+), 1 μl of 10 mM dNTPs, 1 μl of 10 μM upstream primer F, 10 μM of downstream primer R 1 μl, make up to 50 μl with sterile water. The reaction conditions for PCR amplification were: pre-denaturation at 94°C f...
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