Fecal biomarker for diagnosing or preventing diabetes mellitus, detection reagent and application of fecal biomarker

A biomarker and diabetes technology, applied in the field of detection reagents and fecal biomarkers, can solve the problems of increased risk and achieve high specificity

Pending Publication Date: 2022-04-12
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there is an increasing risk of long-term exposure of humans to low-dose radiation

Method used

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  • Fecal biomarker for diagnosing or preventing diabetes mellitus, detection reagent and application of fecal biomarker
  • Fecal biomarker for diagnosing or preventing diabetes mellitus, detection reagent and application of fecal biomarker
  • Fecal biomarker for diagnosing or preventing diabetes mellitus, detection reagent and application of fecal biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] (1) Experiments were carried out with 6-8 week-old healthy mice, and the mice were divided into four experimental groups, with 3 mice in each group, namely: normal control group (Con), low-dose radiation group (LDR), high-dose radiation group (LDR), Sugar-high-fat diet group (HFD), high-sugar and high-fat diet combined with low-dose radiation group (LDR+HFD); the normal control group was not treated, and the mice in the low-dose radiation group were irradiated with cobalt-60 rays once a week at a dose of 0.05Gy, continuous irradiation for 10 weeks, the high-sugar and high-fat diet group was fed with a high-sugar and high-fat diet, and the high-sugar and high-fat diet combined with low-dose radiation group was fed with a high-sugar and high-fat diet and irradiated once a week with cobalt-60 rays at a dose of 0.05Gy, continuous irradiation for 10 weeks. The mice were reared for 3 months.

[0054] (2) Collect feces samples from mice in the four experimental groups, add 90...

Embodiment 2

[0062] This example studies the effects of fecal flora transplantation on HFD (high-sugar and high-fat diet group), LDR+HFD (high-sugar and high-fat diet combined with low-dose radiation group) mice on insulin sensitivity, proline (PA) metabolism and Effect of PYCR1 expression. The mice were transplanted with fecal suspension of healthy control group, HFD case and LDR+HFD case, which were defined as Trans-Con, Trans-HFD and Trans-HFD+LDR, respectively. Image 6 Shown is a timeline of fecal bacterial gavage of mice depleted of gut microbiota antibiotics, in which 5 mice per group were treated with antibiotics in drinking water for 4 days and suspended twice a week with feces. The mice were gavaged with liquid, and tested on the 25th day after the intestinal microbiota antibiotics were depleted. Figure 7-14 The results of blood glucose level, glucose tolerance, insulin tolerance, insulin level, insulin resistance index, fasting blood glucose value, proline level, and lipopolys...

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Abstract

The invention relates to a fecal biomarker for diagnosing or preventing diabetes mellitus, a detection reagent and application of the fecal biomarker. The excrement biomarker for diagnosing or preventing diabetes mellitus provided by the invention comprises one or more of 2-hydroxyhexanoic acid, phenylalanine, valine, acetyltryptophan, nonadecanoic acid, oxalic acid, picolinic acid, alpha-hydroxyisobutyric acid, indoleacetic acid, p-hydroxyphenylacetic acid, acetic acid, thyroxine, 4-hydroxyphenylpyruvic acid, myristic acid or phenylacetic acid. The fecal biomarker provided by the invention is high in sensitivity and specificity and good in predictability, and can be used for early diagnosis or prevention of diabetic people, especially diabetic people exposed by combination of long-term low-dose radiation and high-glucose and high-fat diet.

Description

technical field [0001] The invention designs a stool biomarker, detection reagent and application thereof for diagnosing or preventing diabetes. [0002] technical background [0003] With the widespread application of nuclear power, isotope and radiotherapy, people have more and more opportunities for long-term exposure to low-dose ionizing radiation. At the same time, changes in dietary structure have led to an increase in the number of people with diabetes caused by high-sugar and high-fat diets. The health effects of long-term low-dose radiation combined with high-sugar and high-fat diet have attracted more and more attention. [0004] There are a considerable number of radioactive mineral resources and radioactive mine waste residues on the earth. The various rays released by them, through ionization and excitation, cause long-term potential low-dose radiation hazards to microorganisms, animals, plants, and humans. For example, there are many solid waste storage sites ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/02
Inventor 黄瑞雪周平坤鞠昭
Owner CENT SOUTH UNIV
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