Preparation method of gel for cleaning and nursing female vagina
A vaginal cleansing technology, applied in the field of preparation of women's vaginal cleansing care gel, to achieve the effects of accelerating metabolism, delaying skin aging, and improving skin condition
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Embodiment 1
[0030] The preparation method of white blood cell extract, comprises the following steps:
[0031] S1. Take 50-60 mL of human peripheral blood, and separate peripheral blood mononuclear cells by density gradient separation method of lymphocyte separation medium;
[0032] S2. Wash the peripheral blood mononuclear cells once with the ALys50N-0 medium, at this time the number of peripheral blood mononuclear cells needs to be 3-5×10 7 indivual;
[0033] S3. Adjust the cell concentration to 1-2×10 with ALys50N-0 medium 6 Each / mL was placed in a T175 culture flask and transferred to an incubator for cultivation;
[0034] After S4.2h, take out the T175 culture flask, take another T175 culture flask to collect the suspended cells, add 1000IU / mL IFN-γ for culture;
[0035] S5.24h later, add 50ng / mL anti-CD3 monoclonal antibody, 1000IU / mL IL-2 and 100IU / mL IL-1α for proliferation culture;
[0036] S6. Add double the amount of ALys50N-0 medium every 2-3 days, collect the cell suspens...
Embodiment 2
[0042] A gel for female vagina cleaning and care, prepared from the following ingredients by weight percentage: leukocyte extract 1%, Phellodendron Phellodendron extract 2%, Salvia miltiorrhiza extract 3%, Burnet burnet extract, Sophora flavescens extract 3% liquid, 6% angelica extract, 8% aloe extract, 4% β-nicotinamide mononucleotide, 17% polymer hyaluronic acid, and the balance is purified water.
Embodiment 3
[0044] A cleaning and nursing gel for women's vagina, prepared from the following ingredients by weight percentage: 2.5% leukocyte extract, 4% Phellodendron Phellodendri extract, 3% Danshen extract, 3.5% Burnet extract, Sophora flavescens extract 4% liquid, 8% angelica extract, 12% aloe extract, 3% β-nicotinamide mononucleotide, 17% polymer hyaluronic acid, and the balance is purified water.
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