Traditional Chinese medicine filter pad, preparation method thereof and detection card comprising traditional Chinese medicine filter pad

A filter pad and traditional Chinese medicine technology, which is applied in the field of detection cards, can solve the problems of affecting the results of naked eye observation, blurred background of detection cards, complex components of Chinese medicinal materials, etc., and achieve the effect of easy observation, good adhesion and clear background

Pending Publication Date: 2022-05-27
GUANGZHOU ANNUO FOOD SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition of Chinese herbal medicines and serious matrix interference, it is rarely used in the detection of Chinese herbal medicines, and there are only a few reports in the literature.
[0003] Moreover, before using the test card with Chinese medicinal materials, it is necessary to grind the medicinal materials to prepare a solution. The solution may be mixed with medicinal residues and due to the different types of traditional Chinese medicines, the solution will show different colors, resulting in blurred background of the test card and affecting naked eye observation. result

Method used

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  • Traditional Chinese medicine filter pad, preparation method thereof and detection card comprising traditional Chinese medicine filter pad
  • Traditional Chinese medicine filter pad, preparation method thereof and detection card comprising traditional Chinese medicine filter pad
  • Traditional Chinese medicine filter pad, preparation method thereof and detection card comprising traditional Chinese medicine filter pad

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A detection card, comprising a casing and a bottom plate 1 arranged inside the casing, such as Figures 1 to 2 As shown, the surface of the bottom plate 1 is sequentially provided with a water-absorbing material 2, a chromatography membrane 3, a colloidal gold bonding pad 4 and a traditional Chinese medicine filter pad 5 from top to bottom; wherein, the surface of the chromatography membrane 3 is sequentially provided with qualitative The control line 31 and the detection line 32; the upper shell 6 is provided with an observation window 62 and a sample addition hole 61; ; The traditional Chinese medicine filter pad 5 is prepared by the above-mentioned preparation method of the traditional Chinese medicine filter pad 5. Wherein, the bottom plate 1 is a PVC board. The absorbent material 2 is absorbent paper. The chromatography membrane 3 is a nitrocellulose membrane.

[0031] Wherein, the preparation method of traditional Chinese medicine filter pad 5 comprises the fol...

Embodiment 2

[0034] The structure of the detection card of Embodiment 2 is the same as that of Embodiment 1.

[0035] The preparation method of traditional Chinese medicine filter pad 5, comprises the following steps:

[0036] Soak the base film in the solution for 25 minutes, take it out, and dry it at 60° C. to obtain a traditional Chinese medicine filter pad 5; the solution is a mixture of 100 mL of 0.01M borate buffer, 0.2 g of polyethylene glycol and 0.5 g of sucrose solution. The average molecular weight of polyethylene glycol was 20,000.

Embodiment 3

[0038] The structure of the detection card of Embodiment 3 is the same as that of Embodiment 1.

[0039] The preparation method of traditional Chinese medicine filter pad 5, comprises the following steps:

[0040] Soak the base film in the preparation solution for 10min, take it out, and dry it at 40°C for 12h to obtain a traditional Chinese medicine filter pad 5; the preparation solution is a mixture of 0.01M borate buffer 100mL, 0.2g polyethylene glycol and 0.8g sucrose. mixture. The average molecular weight of polyethylene glycol was 20,000.

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Abstract

The invention discloses a traditional Chinese medicine filter pad, a preparation method thereof and a detection card comprising the traditional Chinese medicine filter pad, the preparation method of the traditional Chinese medicine filter pad is as follows: a base membrane is soaked in a prepared solution, taken out and dried to obtain the traditional Chinese medicine filter pad, and the prepared solution comprises a borate buffer solution and a mixed solution of polyethylene glycol and sugar. Components in the prepared liquid can absorb the color and filter residues of the traditional Chinese medicine liquid to be detected added from the sample hole, so that the background of the observation window is clear after the subsequent traditional Chinese medicine liquid to be detected and colloidal gold are mixed and moved to the chromatography membrane. The traditional Chinese medicine filter pad is additionally arranged below the sample adding hole of the shell on the basis of an existing detection card, so that the color of traditional Chinese medicine liquid to be detected can be effectively filtered, the flowability of the traditional Chinese medicine liquid to be detected cannot be influenced, the background of the observation window is clear, and a detection result is easy to observe.

Description

technical field [0001] The invention relates to the technical field of detection cards, in particular to a traditional Chinese medicine filter pad and a preparation method thereof, and a detection card including the traditional Chinese medicine filter pad. Background technique [0002] Colloidal gold method, also known as colloidal gold immunochromatography assay (GICA), is a detection technology that organically combines various methods such as colloidal gold labeling technology, immunological technology and solid phase chromatography technology. The colloidal gold method is widely used in medical testing, food safety and other fields due to its obvious advantages of simplicity, rapidity, intuitive results, and low cost. Due to the complex composition of Chinese herbal medicines and serious matrix interference, it is rarely used in the detection of Chinese herbal medicines, and there are only a few literature reports. [0003] In addition, before using the test card with C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/548G01N33/58B01D39/14B01D39/16B01D39/18B01D39/20B01J20/26B01J20/28B01J20/32
CPCG01N33/558G01N33/548G01N33/587B01D39/14B01D39/1692B01D39/2068B01D39/16B01D39/18B01J20/28038B01J20/262B01J20/261B01J20/3214B01D2239/10B01D2239/0407B01D2239/0435
Inventor 杨鹏博王军范彬温俊梅周湉
Owner GUANGZHOU ANNUO FOOD SCI & TECH CO LTD
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